Nitroimidazole derivatives: Non-randomness sister chromatid exchanges in human peripheral blood lymphocytes
Nitroimidazole derivatives exhibited genotoxic effect in different experimental conditions. This study focuses on an evaluation of possible genomic targets, at a chromosomal level, of two 5-nitroimidazoles (ornidazole and metronidazole) using the in vitro human peripheral blood culture as experiment...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0260437X_v29_n3_p248_Carballo |
| Aporte de: |
| id |
todo:paper_0260437X_v29_n3_p248_Carballo |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_0260437X_v29_n3_p248_Carballo2023-10-03T15:11:52Z Nitroimidazole derivatives: Non-randomness sister chromatid exchanges in human peripheral blood lymphocytes Carballo, M.A. Martinez, R.A. Mudry, M.D. Biomarkers Chromosomal damage Genomic instability Genotoxicity Nitroimidazoles biological marker metronidazole nitroimidazole derivative ornidazole adult article blood culture cell proliferation chromosome chromosome band chromosome damage chromosome size controlled study cytotoxicity drug exposure female genome analysis genomic instability genotoxicity human human cell human experiment in vitro study male mitosis index normal human peripheral lymphocyte priority journal sister chromatid exchange Biological Markers Cell Division Cells, Cultured Chromosome Banding Dose-Response Relationship, Drug Female Humans Leukocytes, Mononuclear Male Metronidazole Mitotic Index Mutagenicity Tests Mutagens Nitroimidazoles Ornidazole Sister Chromatid Exchange Young Adult Nitroimidazole derivatives exhibited genotoxic effect in different experimental conditions. This study focuses on an evaluation of possible genomic targets, at a chromosomal level, of two 5-nitroimidazoles (ornidazole and metronidazole) using the in vitro human peripheral blood culture as experimental system. We observed that both derivatives showed a decrease in mitotic index (MI) (P < 0.001), an increase in sister chromatid exchanges (SCE) frequency (P < 0.001) and no modifications in cellular proliferation kinetics (CPK). As a null hypothesis we considered the assumption that larger chromosomes should harbor more SCE, which was viewed using a novel sequential G-band (400 band resolution)/SCE technique. The analysis showed highly significant chi square values (P < 0.001), indicating that SCE frequency per chromosome is not proportional to chromosome length. SCE could be considered an instability indicator due to the high correlation between SCEs in certain chromosomal bands and the exposure to nitroimidazole derivatives. Copyright © 2008 John Wiley & Sons, Ltd. Fil:Martinez, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0260437X_v29_n3_p248_Carballo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biomarkers Chromosomal damage Genomic instability Genotoxicity Nitroimidazoles biological marker metronidazole nitroimidazole derivative ornidazole adult article blood culture cell proliferation chromosome chromosome band chromosome damage chromosome size controlled study cytotoxicity drug exposure female genome analysis genomic instability genotoxicity human human cell human experiment in vitro study male mitosis index normal human peripheral lymphocyte priority journal sister chromatid exchange Biological Markers Cell Division Cells, Cultured Chromosome Banding Dose-Response Relationship, Drug Female Humans Leukocytes, Mononuclear Male Metronidazole Mitotic Index Mutagenicity Tests Mutagens Nitroimidazoles Ornidazole Sister Chromatid Exchange Young Adult |
| spellingShingle |
Biomarkers Chromosomal damage Genomic instability Genotoxicity Nitroimidazoles biological marker metronidazole nitroimidazole derivative ornidazole adult article blood culture cell proliferation chromosome chromosome band chromosome damage chromosome size controlled study cytotoxicity drug exposure female genome analysis genomic instability genotoxicity human human cell human experiment in vitro study male mitosis index normal human peripheral lymphocyte priority journal sister chromatid exchange Biological Markers Cell Division Cells, Cultured Chromosome Banding Dose-Response Relationship, Drug Female Humans Leukocytes, Mononuclear Male Metronidazole Mitotic Index Mutagenicity Tests Mutagens Nitroimidazoles Ornidazole Sister Chromatid Exchange Young Adult Carballo, M.A. Martinez, R.A. Mudry, M.D. Nitroimidazole derivatives: Non-randomness sister chromatid exchanges in human peripheral blood lymphocytes |
| topic_facet |
Biomarkers Chromosomal damage Genomic instability Genotoxicity Nitroimidazoles biological marker metronidazole nitroimidazole derivative ornidazole adult article blood culture cell proliferation chromosome chromosome band chromosome damage chromosome size controlled study cytotoxicity drug exposure female genome analysis genomic instability genotoxicity human human cell human experiment in vitro study male mitosis index normal human peripheral lymphocyte priority journal sister chromatid exchange Biological Markers Cell Division Cells, Cultured Chromosome Banding Dose-Response Relationship, Drug Female Humans Leukocytes, Mononuclear Male Metronidazole Mitotic Index Mutagenicity Tests Mutagens Nitroimidazoles Ornidazole Sister Chromatid Exchange Young Adult |
| description |
Nitroimidazole derivatives exhibited genotoxic effect in different experimental conditions. This study focuses on an evaluation of possible genomic targets, at a chromosomal level, of two 5-nitroimidazoles (ornidazole and metronidazole) using the in vitro human peripheral blood culture as experimental system. We observed that both derivatives showed a decrease in mitotic index (MI) (P < 0.001), an increase in sister chromatid exchanges (SCE) frequency (P < 0.001) and no modifications in cellular proliferation kinetics (CPK). As a null hypothesis we considered the assumption that larger chromosomes should harbor more SCE, which was viewed using a novel sequential G-band (400 band resolution)/SCE technique. The analysis showed highly significant chi square values (P < 0.001), indicating that SCE frequency per chromosome is not proportional to chromosome length. SCE could be considered an instability indicator due to the high correlation between SCEs in certain chromosomal bands and the exposure to nitroimidazole derivatives. Copyright © 2008 John Wiley & Sons, Ltd. |
| format |
JOUR |
| author |
Carballo, M.A. Martinez, R.A. Mudry, M.D. |
| author_facet |
Carballo, M.A. Martinez, R.A. Mudry, M.D. |
| author_sort |
Carballo, M.A. |
| title |
Nitroimidazole derivatives: Non-randomness sister chromatid exchanges in human peripheral blood lymphocytes |
| title_short |
Nitroimidazole derivatives: Non-randomness sister chromatid exchanges in human peripheral blood lymphocytes |
| title_full |
Nitroimidazole derivatives: Non-randomness sister chromatid exchanges in human peripheral blood lymphocytes |
| title_fullStr |
Nitroimidazole derivatives: Non-randomness sister chromatid exchanges in human peripheral blood lymphocytes |
| title_full_unstemmed |
Nitroimidazole derivatives: Non-randomness sister chromatid exchanges in human peripheral blood lymphocytes |
| title_sort |
nitroimidazole derivatives: non-randomness sister chromatid exchanges in human peripheral blood lymphocytes |
| url |
http://hdl.handle.net/20.500.12110/paper_0260437X_v29_n3_p248_Carballo |
| work_keys_str_mv |
AT carballoma nitroimidazolederivativesnonrandomnesssisterchromatidexchangesinhumanperipheralbloodlymphocytes AT martinezra nitroimidazolederivativesnonrandomnesssisterchromatidexchangesinhumanperipheralbloodlymphocytes AT mudrymd nitroimidazolederivativesnonrandomnesssisterchromatidexchangesinhumanperipheralbloodlymphocytes |
| _version_ |
1807322049026195456 |