Genotoxic and aneugenic properties of an imidazole derivative
To contribute to a more accurate characterization of the mutagenic and aneugenic effects of thiabendazole (TBZ), a widely used antiparasitic and food preservative drug, the induction of sister chromatid exchanges (SCEs) and mitotic spindle anomalies as cytogenetic end-points were investigated. Studi...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0260437X_v26_n4_p293_Carballo |
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todo:paper_0260437X_v26_n4_p293_Carballo2023-10-03T15:11:51Z Genotoxic and aneugenic properties of an imidazole derivative Carballo, M.A. Hick, A.S. Soloneski, S. Larramendy, M.L. Mudry, M.D. Aneugen Cell proliferation kinetics CHO cells Cytogenetic endpoints Human lymphocytes Mitotic index Mitotic spindle disturbances Sister chromatid exchanges Thiabendazole aneugen antiparasitic agent food preservative imidazole derivative tiabendazole animal cell article cell proliferation CHO cell cytogenetics dose response genotoxicity mitosis index mitosis spindle nonhuman peripheral lymphocyte priority journal sister chromatid exchange Aneugens Animals Cell Proliferation Cell Survival CHO Cells Cricetinae Cricetulus Dose-Response Relationship, Drug Humans Lymphocytes Mitotic Index Mitotic Spindle Apparatus Mutagenicity Tests Sister Chromatid Exchange Thiabendazole Cricetulus griseus To contribute to a more accurate characterization of the mutagenic and aneugenic effects of thiabendazole (TBZ), a widely used antiparasitic and food preservative drug, the induction of sister chromatid exchanges (SCEs) and mitotic spindle anomalies as cytogenetic end-points were investigated. Studies were carried out in Chinese hamster ovary (CHO) cells and human peripheral blood lymphocytes. A significant dose-dependent increase in SCE frequency was observed in CHO cells with S9-Mix (P < 0.01) in the 50-100 μg ml -1 dose-range, while in the absence of S9-Mix, an enhancement of the SCE frequency was exhibited at the highest dose (P < 0.01). In CHO-K1 cells a significant increase in mitotic spindle anomalies (P < 0.01) was observed with the highest concentration assayed reflecting the specific effect of TBZ formulation at the microtubule level. Cell proliferation kinetics (CPK) were not modified by the addition of this pharmaceutical product. In human lymphocyte cultures, exposure to 100 μg ml-1 TBZ formulation resulted in a significant decrease of the mitotic index (MI) (P < 0.003) and changes in the replication index (RI) (P < 0.05). Copyright © 2006 John Wiley & Sons, Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0260437X_v26_n4_p293_Carballo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aneugen Cell proliferation kinetics CHO cells Cytogenetic endpoints Human lymphocytes Mitotic index Mitotic spindle disturbances Sister chromatid exchanges Thiabendazole aneugen antiparasitic agent food preservative imidazole derivative tiabendazole animal cell article cell proliferation CHO cell cytogenetics dose response genotoxicity mitosis index mitosis spindle nonhuman peripheral lymphocyte priority journal sister chromatid exchange Aneugens Animals Cell Proliferation Cell Survival CHO Cells Cricetinae Cricetulus Dose-Response Relationship, Drug Humans Lymphocytes Mitotic Index Mitotic Spindle Apparatus Mutagenicity Tests Sister Chromatid Exchange Thiabendazole Cricetulus griseus |
| spellingShingle |
Aneugen Cell proliferation kinetics CHO cells Cytogenetic endpoints Human lymphocytes Mitotic index Mitotic spindle disturbances Sister chromatid exchanges Thiabendazole aneugen antiparasitic agent food preservative imidazole derivative tiabendazole animal cell article cell proliferation CHO cell cytogenetics dose response genotoxicity mitosis index mitosis spindle nonhuman peripheral lymphocyte priority journal sister chromatid exchange Aneugens Animals Cell Proliferation Cell Survival CHO Cells Cricetinae Cricetulus Dose-Response Relationship, Drug Humans Lymphocytes Mitotic Index Mitotic Spindle Apparatus Mutagenicity Tests Sister Chromatid Exchange Thiabendazole Cricetulus griseus Carballo, M.A. Hick, A.S. Soloneski, S. Larramendy, M.L. Mudry, M.D. Genotoxic and aneugenic properties of an imidazole derivative |
| topic_facet |
Aneugen Cell proliferation kinetics CHO cells Cytogenetic endpoints Human lymphocytes Mitotic index Mitotic spindle disturbances Sister chromatid exchanges Thiabendazole aneugen antiparasitic agent food preservative imidazole derivative tiabendazole animal cell article cell proliferation CHO cell cytogenetics dose response genotoxicity mitosis index mitosis spindle nonhuman peripheral lymphocyte priority journal sister chromatid exchange Aneugens Animals Cell Proliferation Cell Survival CHO Cells Cricetinae Cricetulus Dose-Response Relationship, Drug Humans Lymphocytes Mitotic Index Mitotic Spindle Apparatus Mutagenicity Tests Sister Chromatid Exchange Thiabendazole Cricetulus griseus |
| description |
To contribute to a more accurate characterization of the mutagenic and aneugenic effects of thiabendazole (TBZ), a widely used antiparasitic and food preservative drug, the induction of sister chromatid exchanges (SCEs) and mitotic spindle anomalies as cytogenetic end-points were investigated. Studies were carried out in Chinese hamster ovary (CHO) cells and human peripheral blood lymphocytes. A significant dose-dependent increase in SCE frequency was observed in CHO cells with S9-Mix (P < 0.01) in the 50-100 μg ml -1 dose-range, while in the absence of S9-Mix, an enhancement of the SCE frequency was exhibited at the highest dose (P < 0.01). In CHO-K1 cells a significant increase in mitotic spindle anomalies (P < 0.01) was observed with the highest concentration assayed reflecting the specific effect of TBZ formulation at the microtubule level. Cell proliferation kinetics (CPK) were not modified by the addition of this pharmaceutical product. In human lymphocyte cultures, exposure to 100 μg ml-1 TBZ formulation resulted in a significant decrease of the mitotic index (MI) (P < 0.003) and changes in the replication index (RI) (P < 0.05). Copyright © 2006 John Wiley & Sons, Ltd. |
| format |
JOUR |
| author |
Carballo, M.A. Hick, A.S. Soloneski, S. Larramendy, M.L. Mudry, M.D. |
| author_facet |
Carballo, M.A. Hick, A.S. Soloneski, S. Larramendy, M.L. Mudry, M.D. |
| author_sort |
Carballo, M.A. |
| title |
Genotoxic and aneugenic properties of an imidazole derivative |
| title_short |
Genotoxic and aneugenic properties of an imidazole derivative |
| title_full |
Genotoxic and aneugenic properties of an imidazole derivative |
| title_fullStr |
Genotoxic and aneugenic properties of an imidazole derivative |
| title_full_unstemmed |
Genotoxic and aneugenic properties of an imidazole derivative |
| title_sort |
genotoxic and aneugenic properties of an imidazole derivative |
| url |
http://hdl.handle.net/20.500.12110/paper_0260437X_v26_n4_p293_Carballo |
| work_keys_str_mv |
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| _version_ |
1807316346422165504 |