Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions
Aggregation is a common trait of bacteria in natural and engineered biological systems. Microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. It is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmen...
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todo:paper_0233111X_v55_n9_p1134_Ayarza2023-10-03T15:11:19Z Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions Ayarza, J.M. Mazzella, M.A. Erijman, L. Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes culture medium isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Bacteroidetes Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome Aggregation is a common trait of bacteria in natural and engineered biological systems. Microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. It is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmental stress better than free-swimming bacteria of the same species. We performed a proteomic analysis of a strain of Sediminibacterium, isolated from activated sludge, which grew planktonically in diluted culture media and in an aggregated form in media containing a high concentration of organic substrate. Auto-aggregation was also observed in the presence of pyruvate in dilute media. Expression of a number of stress-related proteins significantly increased under planktonic growth in comparison to aggregate growth. The upregulated proteins, identified by MALDI-TOF mass spectrometry, were two isoforms of a protein belonging to the universal stress family (UspA), a thioredoxin-disulfide reductase, the Campylobacter jejuni orthologue transcriptional regulator (Cj1172c), and the CocE/NonD hydrolase. We conclude that Sediminibaterium sp. C3 growth is stressed under planktonic conditions and that aggregation induced by pyruvate protects the bacteria against oxidative stress. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Fil:Mazzella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0233111X_v55_n9_p1134_Ayarza |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes culture medium isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Bacteroidetes Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome |
| spellingShingle |
Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes culture medium isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Bacteroidetes Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome Ayarza, J.M. Mazzella, M.A. Erijman, L. Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions |
| topic_facet |
Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes culture medium isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Bacteroidetes Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome |
| description |
Aggregation is a common trait of bacteria in natural and engineered biological systems. Microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. It is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmental stress better than free-swimming bacteria of the same species. We performed a proteomic analysis of a strain of Sediminibacterium, isolated from activated sludge, which grew planktonically in diluted culture media and in an aggregated form in media containing a high concentration of organic substrate. Auto-aggregation was also observed in the presence of pyruvate in dilute media. Expression of a number of stress-related proteins significantly increased under planktonic growth in comparison to aggregate growth. The upregulated proteins, identified by MALDI-TOF mass spectrometry, were two isoforms of a protein belonging to the universal stress family (UspA), a thioredoxin-disulfide reductase, the Campylobacter jejuni orthologue transcriptional regulator (Cj1172c), and the CocE/NonD hydrolase. We conclude that Sediminibaterium sp. C3 growth is stressed under planktonic conditions and that aggregation induced by pyruvate protects the bacteria against oxidative stress. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| format |
JOUR |
| author |
Ayarza, J.M. Mazzella, M.A. Erijman, L. |
| author_facet |
Ayarza, J.M. Mazzella, M.A. Erijman, L. |
| author_sort |
Ayarza, J.M. |
| title |
Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions |
| title_short |
Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions |
| title_full |
Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions |
| title_fullStr |
Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions |
| title_full_unstemmed |
Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions |
| title_sort |
expression of stress-related proteins in sediminibacterium sp. growing under planktonic conditions |
| url |
http://hdl.handle.net/20.500.12110/paper_0233111X_v55_n9_p1134_Ayarza |
| work_keys_str_mv |
AT ayarzajm expressionofstressrelatedproteinsinsediminibacteriumspgrowingunderplanktonicconditions AT mazzellama expressionofstressrelatedproteinsinsediminibacteriumspgrowingunderplanktonicconditions AT erijmanl expressionofstressrelatedproteinsinsediminibacteriumspgrowingunderplanktonicconditions |
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1807321797110005760 |