Amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab Neohelice granulata (Decapoda: Brachyura: Varunidae): Partial characterization and response to low environmental salinity

Studies on digestive adjustments at the biochemical level in relation to salinity in euryhaline crabs are lacking. Moreover, knowledge of biochemical digestive characteristics of euryhaline crabs (i.e. occurrence and characteristics of key digestive enzyme activities) is still scarce and fragmentary...

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Autores principales: Asaro, A., del Valle, J.C., Mañanes, A.A.L.
Formato: JOUR
Lenguaje:English; Spanish
Materias:
Amylase
Euryhaline crabs
Hepatopancreas
Hyperregulation
Maltase
Sucrase
acclimation
biochemical composition
crab
digestion
environmental factor
enzyme activity
pH
physiological response
salinity
Brachyura
Chasmagnathus
Decapoda (Crustacea)
Varunidae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02148358_v75_n3_p517_Asaro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_02148358_v75_n3_p517_Asaro2023-10-03T15:10:17Z Amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab Neohelice granulata (Decapoda: Brachyura: Varunidae): Partial characterization and response to low environmental salinity Asaro, A. del Valle, J.C. Mañanes, A.A.L. Amylase Euryhaline crabs Hepatopancreas Hyperregulation Maltase Sucrase acclimation biochemical composition crab digestion environmental factor enzyme activity pH physiological response salinity Brachyura Chasmagnathus Decapoda (Crustacea) Varunidae Studies on digestive adjustments at the biochemical level in relation to salinity in euryhaline crabs are lacking. Moreover, knowledge of biochemical digestive characteristics of euryhaline crabs (i.e. occurrence and characteristics of key digestive enzyme activities) is still scarce and fragmentary. We studied the occurrence, characteristics and response to low salinity of amylase, maltase and sucrase activities in the hepatopancreas of the euryhaline crab Neohelice (Chasmagnathus) granulata. Maximal amylase and maltase activities were found at pH 5.2. Sucrase activity was maximal within the pH range 3.6-5.2. Amylase, maltase and sucrase activities showed a Michaelis-Menten kinetics (km = 0.41±0.10 mg ml-1; 1.37±1.03 mM and 0.55±0.45 mM, respectively). In crabs acclimated to low salinity (10 psu; hyperregulating conditions), amylase activity (7263±980 μg maltose min-1 mg prot-1) was higher than in 35 psu (osmoconforming conditions) (3605±340 μg maltose min-1 mg prot-1). Maltase and sucrase activities (497±98 and 64±16 μg glucose min-1 mg prot-1, respectively) were similar in both salinities. The response of amylase activity to low salinity suggests a role in digestive adjustments upon hyperregulation. This study contributes to a better understanding of the complexity of the biochemical adaptations to low salinity in euryhaline crabs. JOUR English; Spanish info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02148358_v75_n3_p517_Asaro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language English; Spanish
orig_language_str_mv English; Spanish
topic Amylase
Euryhaline crabs
Hepatopancreas
Hyperregulation
Maltase
Sucrase
acclimation
biochemical composition
crab
digestion
environmental factor
enzyme activity
pH
physiological response
salinity
Brachyura
Chasmagnathus
Decapoda (Crustacea)
Varunidae
spellingShingle Amylase
Euryhaline crabs
Hepatopancreas
Hyperregulation
Maltase
Sucrase
acclimation
biochemical composition
crab
digestion
environmental factor
enzyme activity
pH
physiological response
salinity
Brachyura
Chasmagnathus
Decapoda (Crustacea)
Varunidae
Asaro, A.
del Valle, J.C.
Mañanes, A.A.L.
Amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab Neohelice granulata (Decapoda: Brachyura: Varunidae): Partial characterization and response to low environmental salinity
topic_facet Amylase
Euryhaline crabs
Hepatopancreas
Hyperregulation
Maltase
Sucrase
acclimation
biochemical composition
crab
digestion
environmental factor
enzyme activity
pH
physiological response
salinity
Brachyura
Chasmagnathus
Decapoda (Crustacea)
Varunidae
description Studies on digestive adjustments at the biochemical level in relation to salinity in euryhaline crabs are lacking. Moreover, knowledge of biochemical digestive characteristics of euryhaline crabs (i.e. occurrence and characteristics of key digestive enzyme activities) is still scarce and fragmentary. We studied the occurrence, characteristics and response to low salinity of amylase, maltase and sucrase activities in the hepatopancreas of the euryhaline crab Neohelice (Chasmagnathus) granulata. Maximal amylase and maltase activities were found at pH 5.2. Sucrase activity was maximal within the pH range 3.6-5.2. Amylase, maltase and sucrase activities showed a Michaelis-Menten kinetics (km = 0.41±0.10 mg ml-1; 1.37±1.03 mM and 0.55±0.45 mM, respectively). In crabs acclimated to low salinity (10 psu; hyperregulating conditions), amylase activity (7263±980 μg maltose min-1 mg prot-1) was higher than in 35 psu (osmoconforming conditions) (3605±340 μg maltose min-1 mg prot-1). Maltase and sucrase activities (497±98 and 64±16 μg glucose min-1 mg prot-1, respectively) were similar in both salinities. The response of amylase activity to low salinity suggests a role in digestive adjustments upon hyperregulation. This study contributes to a better understanding of the complexity of the biochemical adaptations to low salinity in euryhaline crabs.
format JOUR
author Asaro, A.
del Valle, J.C.
Mañanes, A.A.L.
author_facet Asaro, A.
del Valle, J.C.
Mañanes, A.A.L.
author_sort Asaro, A.
title Amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab Neohelice granulata (Decapoda: Brachyura: Varunidae): Partial characterization and response to low environmental salinity
title_short Amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab Neohelice granulata (Decapoda: Brachyura: Varunidae): Partial characterization and response to low environmental salinity
title_full Amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab Neohelice granulata (Decapoda: Brachyura: Varunidae): Partial characterization and response to low environmental salinity
title_fullStr Amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab Neohelice granulata (Decapoda: Brachyura: Varunidae): Partial characterization and response to low environmental salinity
title_full_unstemmed Amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab Neohelice granulata (Decapoda: Brachyura: Varunidae): Partial characterization and response to low environmental salinity
title_sort amylase, maltase and sucrase activities in hepatopancreas of the euryhaline crab neohelice granulata (decapoda: brachyura: varunidae): partial characterization and response to low environmental salinity
url http://hdl.handle.net/20.500.12110/paper_02148358_v75_n3_p517_Asaro
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AT delvallejc amylasemaltaseandsucraseactivitiesinhepatopancreasoftheeuryhalinecrabneohelicegranulatadecapodabrachyuravarunidaepartialcharacterizationandresponsetolowenvironmentalsalinity
AT mananesaal amylasemaltaseandsucraseactivitiesinhepatopancreasoftheeuryhalinecrabneohelicegranulatadecapodabrachyuravarunidaepartialcharacterizationandresponsetolowenvironmentalsalinity
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