Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones
Background. Chlamydia trachomatis is the most prevalent bacteria causing sexually transmitted infections. In women, this infection can cause cervicitis and urethritis, although it’s usually asymptomatic. The aim of this study was to investigate the prevalence of C. trachomatis in women attending the...
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todo:paper_02143429_v31_n1_p21_Jorda2023-10-03T15:10:13Z Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones Jordá, G.B. Hanke, S.E. Ramos-Rincón, J.M. Mosmann, J. Lopéz, M.L. Entrocassi, A.C. Cuffini, C. Chlamydia trachomatis Diagnostic Genotypes PCR Prevalence adult Article bacterial strain Chlamydia trachomatis chlamydiasis controlled study female gene gene amplification genetic analysis genotype human human tissue major clinical study molecular epidemiology nonhuman ompA gene phylogeny plasmid polymerase chain reaction prevalence Background. Chlamydia trachomatis is the most prevalent bacteria causing sexually transmitted infections. In women, this infection can cause cervicitis and urethritis, although it’s usually asymptomatic. The aim of this study was to investigate the prevalence of C. trachomatis in women attending the lab Instituto de Previsión Social and detect the genotypes. Material and methods. Endocervical samples from 505 symptomatic and asymptomatic women were assayed. It was determined the presence of C. trachomatis by PCR through amplification of a fragment of the cryptic plasmid. Positive samples were genotyped by the partial amplification of the ompA gene and analyzed phylogenetically. Results. Forty-three positive samples were detected to infection with C. trachomatis, obtaining a prevalence of 8.5% (IC 95%: 6.4-11.3%). The prevalence of C. trachomatis was higher in women with vaginal symptoms [11.3% (30/265) vs. 5.4% (13/240)] (p = 0.018), as well as in women under 26 year-old [11.5% (28/244) vs. 6.2% (15/246)] (p = 0.021). Based on phylogenetic analysis, it was observed that 62% of the samples were genotype E, 15% genotype J, 15% genotype D, and 8% genotype F. Conclusions. This work is the first contribution on the molecular epidemiology of C. trachomatis in the Misiones province, Argentina, which shows the rate of prevalence of this bacterium and offers information on circulating genotypes. © 2018, Sociedad Espanola de Quiminoterapia. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02143429_v31_n1_p21_Jorda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chlamydia trachomatis Diagnostic Genotypes PCR Prevalence adult Article bacterial strain Chlamydia trachomatis chlamydiasis controlled study female gene gene amplification genetic analysis genotype human human tissue major clinical study molecular epidemiology nonhuman ompA gene phylogeny plasmid polymerase chain reaction prevalence |
| spellingShingle |
Chlamydia trachomatis Diagnostic Genotypes PCR Prevalence adult Article bacterial strain Chlamydia trachomatis chlamydiasis controlled study female gene gene amplification genetic analysis genotype human human tissue major clinical study molecular epidemiology nonhuman ompA gene phylogeny plasmid polymerase chain reaction prevalence Jordá, G.B. Hanke, S.E. Ramos-Rincón, J.M. Mosmann, J. Lopéz, M.L. Entrocassi, A.C. Cuffini, C. Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones |
| topic_facet |
Chlamydia trachomatis Diagnostic Genotypes PCR Prevalence adult Article bacterial strain Chlamydia trachomatis chlamydiasis controlled study female gene gene amplification genetic analysis genotype human human tissue major clinical study molecular epidemiology nonhuman ompA gene phylogeny plasmid polymerase chain reaction prevalence |
| description |
Background. Chlamydia trachomatis is the most prevalent bacteria causing sexually transmitted infections. In women, this infection can cause cervicitis and urethritis, although it’s usually asymptomatic. The aim of this study was to investigate the prevalence of C. trachomatis in women attending the lab Instituto de Previsión Social and detect the genotypes. Material and methods. Endocervical samples from 505 symptomatic and asymptomatic women were assayed. It was determined the presence of C. trachomatis by PCR through amplification of a fragment of the cryptic plasmid. Positive samples were genotyped by the partial amplification of the ompA gene and analyzed phylogenetically. Results. Forty-three positive samples were detected to infection with C. trachomatis, obtaining a prevalence of 8.5% (IC 95%: 6.4-11.3%). The prevalence of C. trachomatis was higher in women with vaginal symptoms [11.3% (30/265) vs. 5.4% (13/240)] (p = 0.018), as well as in women under 26 year-old [11.5% (28/244) vs. 6.2% (15/246)] (p = 0.021). Based on phylogenetic analysis, it was observed that 62% of the samples were genotype E, 15% genotype J, 15% genotype D, and 8% genotype F. Conclusions. This work is the first contribution on the molecular epidemiology of C. trachomatis in the Misiones province, Argentina, which shows the rate of prevalence of this bacterium and offers information on circulating genotypes. © 2018, Sociedad Espanola de Quiminoterapia. All rights reserved. |
| format |
JOUR |
| author |
Jordá, G.B. Hanke, S.E. Ramos-Rincón, J.M. Mosmann, J. Lopéz, M.L. Entrocassi, A.C. Cuffini, C. |
| author_facet |
Jordá, G.B. Hanke, S.E. Ramos-Rincón, J.M. Mosmann, J. Lopéz, M.L. Entrocassi, A.C. Cuffini, C. |
| author_sort |
Jordá, G.B. |
| title |
Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones |
| title_short |
Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones |
| title_full |
Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones |
| title_fullStr |
Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones |
| title_full_unstemmed |
Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones |
| title_sort |
prevalence and phylogenetic analysis of chlamydia trachomatis in a population of women in posadas, misiones |
| url |
http://hdl.handle.net/20.500.12110/paper_02143429_v31_n1_p21_Jorda |
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