High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies
Poor-quality sperm show reduced capacity to under-go capacitation-induced protein tyrosine phosphorylation and hyperactivation. Given that these deficiencies can be overcome by membrane-permeant stimulators of the cAMP-dependent kinase system, we hypothesize that the main defect underlying these def...
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todo:paper_01963635_v30_n5_p552_Buffone2023-10-03T15:09:45Z High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies Buffone, M.G. Verstraeten, S.V. Calamera, J.C. Doncel, G.F. Capacitation Hyperactivation Plasma membrane cholesterol desmosterol article cell membrane controlled study human human experiment male membrane fluidity normal human priority journal protein phosphorylation seminal plasma spermatozoon spermatozoon capacitation spermatozoon motility Cell Membrane Cholesterol Desmosterol Humans Male Membrane Fluidity Phosphorylation Sperm Capacitation Sperm Motility Spermatozoa Tyrosine Poor-quality sperm show reduced capacity to under-go capacitation-induced protein tyrosine phosphorylation and hyperactivation. Given that these deficiencies can be overcome by membrane-permeant stimulators of the cAMP-dependent kinase system, we hypothesize that the main defect underlying these deficiencies resides on the sperm plasma membrane. Spermatozoa from semen samples obtained from 15 consenting healthy donors were separated in 2 subpopulations, L45 (first interface) and L90 (pellet), using a 45:65:90 isolate gradient centrifugation method. These sperm fractions were studied before and after a 6-hour capacitating incubation for sperm motion parameters (computer-assisted analysis), including hyperactivation, protein tyrosine phos-phorylation (immunofluorescence), membrane fluidity (Laurdan fluorescence), and sterol and phospholipid content (high-performance thin-layer chromatography). In summary, data indicate that L45 (poor-motility) spermatozoa present an excess of cholesterol and desmosterol, which impairs the normal increase in membrane fluidity during capacitation and its consequent activation of protein tyrosine phosphorylation and hypermotility. Therefore, a defect in membrane composition and dynamics is underlying human sperm biochemical and functional deficiencies related to inadequate capacitation. Copyright © American Society of Andrology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01963635_v30_n5_p552_Buffone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Capacitation Hyperactivation Plasma membrane cholesterol desmosterol article cell membrane controlled study human human experiment male membrane fluidity normal human priority journal protein phosphorylation seminal plasma spermatozoon spermatozoon capacitation spermatozoon motility Cell Membrane Cholesterol Desmosterol Humans Male Membrane Fluidity Phosphorylation Sperm Capacitation Sperm Motility Spermatozoa Tyrosine |
| spellingShingle |
Capacitation Hyperactivation Plasma membrane cholesterol desmosterol article cell membrane controlled study human human experiment male membrane fluidity normal human priority journal protein phosphorylation seminal plasma spermatozoon spermatozoon capacitation spermatozoon motility Cell Membrane Cholesterol Desmosterol Humans Male Membrane Fluidity Phosphorylation Sperm Capacitation Sperm Motility Spermatozoa Tyrosine Buffone, M.G. Verstraeten, S.V. Calamera, J.C. Doncel, G.F. High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies |
| topic_facet |
Capacitation Hyperactivation Plasma membrane cholesterol desmosterol article cell membrane controlled study human human experiment male membrane fluidity normal human priority journal protein phosphorylation seminal plasma spermatozoon spermatozoon capacitation spermatozoon motility Cell Membrane Cholesterol Desmosterol Humans Male Membrane Fluidity Phosphorylation Sperm Capacitation Sperm Motility Spermatozoa Tyrosine |
| description |
Poor-quality sperm show reduced capacity to under-go capacitation-induced protein tyrosine phosphorylation and hyperactivation. Given that these deficiencies can be overcome by membrane-permeant stimulators of the cAMP-dependent kinase system, we hypothesize that the main defect underlying these deficiencies resides on the sperm plasma membrane. Spermatozoa from semen samples obtained from 15 consenting healthy donors were separated in 2 subpopulations, L45 (first interface) and L90 (pellet), using a 45:65:90 isolate gradient centrifugation method. These sperm fractions were studied before and after a 6-hour capacitating incubation for sperm motion parameters (computer-assisted analysis), including hyperactivation, protein tyrosine phos-phorylation (immunofluorescence), membrane fluidity (Laurdan fluorescence), and sterol and phospholipid content (high-performance thin-layer chromatography). In summary, data indicate that L45 (poor-motility) spermatozoa present an excess of cholesterol and desmosterol, which impairs the normal increase in membrane fluidity during capacitation and its consequent activation of protein tyrosine phosphorylation and hypermotility. Therefore, a defect in membrane composition and dynamics is underlying human sperm biochemical and functional deficiencies related to inadequate capacitation. Copyright © American Society of Andrology. |
| format |
JOUR |
| author |
Buffone, M.G. Verstraeten, S.V. Calamera, J.C. Doncel, G.F. |
| author_facet |
Buffone, M.G. Verstraeten, S.V. Calamera, J.C. Doncel, G.F. |
| author_sort |
Buffone, M.G. |
| title |
High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies |
| title_short |
High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies |
| title_full |
High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies |
| title_fullStr |
High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies |
| title_full_unstemmed |
High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies |
| title_sort |
high cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies |
| url |
http://hdl.handle.net/20.500.12110/paper_01963635_v30_n5_p552_Buffone |
| work_keys_str_mv |
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