Characterization of L-arginine transport in adrenal cells: Effect of ACTH
Nitric oxide synthesis depends on the availability of its precursor L-arginine, which could be regulated by the presence of a specific uptake system. In the present report, the characterization of the L-arginine transport system in mouse adrenal Y1 cells was performed. L-arginine transport was media...
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todo:paper_01931849_v291_n2_pE291_Repetto2023-10-03T15:09:14Z Characterization of L-arginine transport in adrenal cells: Effect of ACTH Repetto, E.M. Pannunzio, V. Astort, F. Calejman, C.M. Moreno, M.B. Pignataro, O.P. Cymeryng, C.B. Adrenocorticotropic hormone Nitric oxide Protein kinase A amino acid transporter arginine CD98 antigen corticotropin cyclic AMP n ethylmaleimide nitric oxide sodium ion adrenal cell amino acid analysis amino acid transport animal cell article controlled study drug effect gene expression mouse nonhuman nucleotide sequence priority journal protein expression reverse transcription polymerase chain reaction sodium transport upregulation zona fasciculata Adrenal Cortex Adrenocorticotropic Hormone Amino Acid Transport Systems, Basic Animals Arginine Biological Transport, Active Cell Line Cyclic AMP Dose-Response Relationship, Drug Mice Nitric Oxide Rats Nitric oxide synthesis depends on the availability of its precursor L-arginine, which could be regulated by the presence of a specific uptake system. In the present report, the characterization of the L-arginine transport system in mouse adrenal Y1 cells was performed. L-arginine transport was mediated by the cationic/neutral amino acid transport system y+L and the cationic amino acid transporter (CAT) y+ in Y1 cells. These Na+-independent transporters were identified by their selectivity for neutral amino acids in both the presence and absence of Na+ and by the effect of N-ethylmaleimide. Transport data correlated to expression of genes encoding for CAT-1, CAT-2, CD-98, and y+LAT-2. A similar expression profile was detected in rat adrenal zona fasciculata. In addition, cationic amino acid uptake in Y1 cells was upregulated by ACTH and/or cAMP with a concomitant increase in nitric oxide (NO) production. Copyright © 2006 the American Physiological Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01931849_v291_n2_pE291_Repetto |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adrenocorticotropic hormone Nitric oxide Protein kinase A amino acid transporter arginine CD98 antigen corticotropin cyclic AMP n ethylmaleimide nitric oxide sodium ion adrenal cell amino acid analysis amino acid transport animal cell article controlled study drug effect gene expression mouse nonhuman nucleotide sequence priority journal protein expression reverse transcription polymerase chain reaction sodium transport upregulation zona fasciculata Adrenal Cortex Adrenocorticotropic Hormone Amino Acid Transport Systems, Basic Animals Arginine Biological Transport, Active Cell Line Cyclic AMP Dose-Response Relationship, Drug Mice Nitric Oxide Rats |
| spellingShingle |
Adrenocorticotropic hormone Nitric oxide Protein kinase A amino acid transporter arginine CD98 antigen corticotropin cyclic AMP n ethylmaleimide nitric oxide sodium ion adrenal cell amino acid analysis amino acid transport animal cell article controlled study drug effect gene expression mouse nonhuman nucleotide sequence priority journal protein expression reverse transcription polymerase chain reaction sodium transport upregulation zona fasciculata Adrenal Cortex Adrenocorticotropic Hormone Amino Acid Transport Systems, Basic Animals Arginine Biological Transport, Active Cell Line Cyclic AMP Dose-Response Relationship, Drug Mice Nitric Oxide Rats Repetto, E.M. Pannunzio, V. Astort, F. Calejman, C.M. Moreno, M.B. Pignataro, O.P. Cymeryng, C.B. Characterization of L-arginine transport in adrenal cells: Effect of ACTH |
| topic_facet |
Adrenocorticotropic hormone Nitric oxide Protein kinase A amino acid transporter arginine CD98 antigen corticotropin cyclic AMP n ethylmaleimide nitric oxide sodium ion adrenal cell amino acid analysis amino acid transport animal cell article controlled study drug effect gene expression mouse nonhuman nucleotide sequence priority journal protein expression reverse transcription polymerase chain reaction sodium transport upregulation zona fasciculata Adrenal Cortex Adrenocorticotropic Hormone Amino Acid Transport Systems, Basic Animals Arginine Biological Transport, Active Cell Line Cyclic AMP Dose-Response Relationship, Drug Mice Nitric Oxide Rats |
| description |
Nitric oxide synthesis depends on the availability of its precursor L-arginine, which could be regulated by the presence of a specific uptake system. In the present report, the characterization of the L-arginine transport system in mouse adrenal Y1 cells was performed. L-arginine transport was mediated by the cationic/neutral amino acid transport system y+L and the cationic amino acid transporter (CAT) y+ in Y1 cells. These Na+-independent transporters were identified by their selectivity for neutral amino acids in both the presence and absence of Na+ and by the effect of N-ethylmaleimide. Transport data correlated to expression of genes encoding for CAT-1, CAT-2, CD-98, and y+LAT-2. A similar expression profile was detected in rat adrenal zona fasciculata. In addition, cationic amino acid uptake in Y1 cells was upregulated by ACTH and/or cAMP with a concomitant increase in nitric oxide (NO) production. Copyright © 2006 the American Physiological Society. |
| format |
JOUR |
| author |
Repetto, E.M. Pannunzio, V. Astort, F. Calejman, C.M. Moreno, M.B. Pignataro, O.P. Cymeryng, C.B. |
| author_facet |
Repetto, E.M. Pannunzio, V. Astort, F. Calejman, C.M. Moreno, M.B. Pignataro, O.P. Cymeryng, C.B. |
| author_sort |
Repetto, E.M. |
| title |
Characterization of L-arginine transport in adrenal cells: Effect of ACTH |
| title_short |
Characterization of L-arginine transport in adrenal cells: Effect of ACTH |
| title_full |
Characterization of L-arginine transport in adrenal cells: Effect of ACTH |
| title_fullStr |
Characterization of L-arginine transport in adrenal cells: Effect of ACTH |
| title_full_unstemmed |
Characterization of L-arginine transport in adrenal cells: Effect of ACTH |
| title_sort |
characterization of l-arginine transport in adrenal cells: effect of acth |
| url |
http://hdl.handle.net/20.500.12110/paper_01931849_v291_n2_pE291_Repetto |
| work_keys_str_mv |
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| _version_ |
1807322700083888128 |