Angiotensin II-induced Ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells

We evaluated the effects of angiotensin II (ANG II) and its antagonists on prolactin release, intracellular calcium ([Ca2+](i)) mobilization, and [3H]thymidine uptake in cells from normal rat pituitaries and from estrogen- induced pituitary tumors. ANG II (10-7 to 10-9 M) increased prolactin release...

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Autores principales: Díaz-Torga, G., Iglesias, A.G., Achával-Zaia, R., Libertun, C., Becú-Villalobos, D.
Formato: JOUR
Materias:
Calcium
Estrogen
angiotensin
angiogenesis
animal cell
article
calcium mobilization
cell hyperplasia
DNA synthesis inhibition
drug receptor binding
enzyme activation
hormone release
hypophysis cell
immediate early gene
nonhuman
priority journal
prolactin release
promoter region
rat
renin angiotensin aldosterone system
tumor growth
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01931849_v274_n337-3_pE534_DiazTorga
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_01931849_v274_n337-3_pE534_DiazTorga2023-10-03T15:09:12Z Angiotensin II-induced Ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells Díaz-Torga, G. Iglesias, A.G. Achával-Zaia, R. Libertun, C. Becú-Villalobos, D. Calcium Estrogen angiotensin angiogenesis animal cell article calcium mobilization cell hyperplasia DNA synthesis inhibition drug receptor binding enzyme activation hormone release hypophysis cell immediate early gene nonhuman priority journal prolactin release promoter region rat renin angiotensin aldosterone system tumor growth We evaluated the effects of angiotensin II (ANG II) and its antagonists on prolactin release, intracellular calcium ([Ca2+](i)) mobilization, and [3H]thymidine uptake in cells from normal rat pituitaries and from estrogen- induced pituitary tumors. ANG II (10-7 to 10-9 M) increased prolactin release significantly in control and not in tumoral cells. In control cells, ANG II (10-6 to 10-9 M) produced an immediate spike of [Ca2+](i) followed by a plateau. Spike levels rose significantly between 10-10 and 10-8 M ANG II, whereas the onset of the spike was retarded with decreasing concentrations. In tumoral cells, ANG II did not produce a spike phase even at 10-6 M. ANG II-induced prolactin release and calcium mobilization were blocked by losartan (AT1 receptor antagonist) and not by PD-123319 (AT2 antagonist). Finally, [3H]thymidine uptake was not modified by ANG II (10- 7 to 10-10 M) or its antagonists in either group. Our results suggest that chronic in vivo estrogenic treatment alters in vitro pituitary response to ANG II. Alterations might function to limit excessive prolactin secretion of hypersecreting tumors. Besides, ANG II does not modify DNA synthesis in vitro of cells from normal or tumor-derived hypophyses. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01931849_v274_n337-3_pE534_DiazTorga
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Calcium
Estrogen
angiotensin
angiogenesis
animal cell
article
calcium mobilization
cell hyperplasia
DNA synthesis inhibition
drug receptor binding
enzyme activation
hormone release
hypophysis cell
immediate early gene
nonhuman
priority journal
prolactin release
promoter region
rat
renin angiotensin aldosterone system
tumor growth
spellingShingle Calcium
Estrogen
angiotensin
angiogenesis
animal cell
article
calcium mobilization
cell hyperplasia
DNA synthesis inhibition
drug receptor binding
enzyme activation
hormone release
hypophysis cell
immediate early gene
nonhuman
priority journal
prolactin release
promoter region
rat
renin angiotensin aldosterone system
tumor growth
Díaz-Torga, G.
Iglesias, A.G.
Achával-Zaia, R.
Libertun, C.
Becú-Villalobos, D.
Angiotensin II-induced Ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells
topic_facet Calcium
Estrogen
angiotensin
angiogenesis
animal cell
article
calcium mobilization
cell hyperplasia
DNA synthesis inhibition
drug receptor binding
enzyme activation
hormone release
hypophysis cell
immediate early gene
nonhuman
priority journal
prolactin release
promoter region
rat
renin angiotensin aldosterone system
tumor growth
description We evaluated the effects of angiotensin II (ANG II) and its antagonists on prolactin release, intracellular calcium ([Ca2+](i)) mobilization, and [3H]thymidine uptake in cells from normal rat pituitaries and from estrogen- induced pituitary tumors. ANG II (10-7 to 10-9 M) increased prolactin release significantly in control and not in tumoral cells. In control cells, ANG II (10-6 to 10-9 M) produced an immediate spike of [Ca2+](i) followed by a plateau. Spike levels rose significantly between 10-10 and 10-8 M ANG II, whereas the onset of the spike was retarded with decreasing concentrations. In tumoral cells, ANG II did not produce a spike phase even at 10-6 M. ANG II-induced prolactin release and calcium mobilization were blocked by losartan (AT1 receptor antagonist) and not by PD-123319 (AT2 antagonist). Finally, [3H]thymidine uptake was not modified by ANG II (10- 7 to 10-10 M) or its antagonists in either group. Our results suggest that chronic in vivo estrogenic treatment alters in vitro pituitary response to ANG II. Alterations might function to limit excessive prolactin secretion of hypersecreting tumors. Besides, ANG II does not modify DNA synthesis in vitro of cells from normal or tumor-derived hypophyses.
format JOUR
author Díaz-Torga, G.
Iglesias, A.G.
Achával-Zaia, R.
Libertun, C.
Becú-Villalobos, D.
author_facet Díaz-Torga, G.
Iglesias, A.G.
Achával-Zaia, R.
Libertun, C.
Becú-Villalobos, D.
author_sort Díaz-Torga, G.
title Angiotensin II-induced Ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells
title_short Angiotensin II-induced Ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells
title_full Angiotensin II-induced Ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells
title_fullStr Angiotensin II-induced Ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells
title_full_unstemmed Angiotensin II-induced Ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells
title_sort angiotensin ii-induced ca2+ mobilization and prolactin release in normal and hyperplastic pituitary cells
url http://hdl.handle.net/20.500.12110/paper_01931849_v274_n337-3_pE534_DiazTorga
work_keys_str_mv AT diaztorgag angiotensiniiinducedca2mobilizationandprolactinreleaseinnormalandhyperplasticpituitarycells
AT iglesiasag angiotensiniiinducedca2mobilizationandprolactinreleaseinnormalandhyperplasticpituitarycells
AT achavalzaiar angiotensiniiinducedca2mobilizationandprolactinreleaseinnormalandhyperplasticpituitarycells
AT libertunc angiotensiniiinducedca2mobilizationandprolactinreleaseinnormalandhyperplasticpituitarycells
AT becuvillalobosd angiotensiniiinducedca2mobilizationandprolactinreleaseinnormalandhyperplasticpituitarycells
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