Alloimmunization-induced changes in thymocytes' prostaglandin levels: A signal for the induction of biological activity

The effect of BALB/c anti C3H non-adherent thymocytes on the spontaneous activity of isolated C3H mouse atria was studied. Immune non-adherent thymocytes alone induced negative inotropic action, while macrophages alone did not have any effect. The same kind of collaborative effect between both cells...

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Autores principales: Borda, E., Genaro, A., Peredo, H., Cremaschi, G., Leiros, C.P., Sterin-Borda, L.
Formato: JOUR
Materias:
acetylsalicylic acid
arachidonic acid
indometacin
prostaglandin
prostaglandin e2
animal cell
cytology
drug administration
drug mechanism
drug monitoring
heart
heart atrium
heart muscle contractility
immunization
macrophage
mouse
nonhuman
preliminary communication
priority journal
thymocyte
thymus
Animals
Arachidonic Acid
Arachidonic Acids
Immunization
Macrophages
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Myocardial Contraction
Prostaglandins E
T-Lymphocytes
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01920561_v7_n5_p647_Borda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01920561_v7_n5_p647_Borda
record_format dspace
spelling todo:paper_01920561_v7_n5_p647_Borda2023-10-03T15:09:06Z Alloimmunization-induced changes in thymocytes' prostaglandin levels: A signal for the induction of biological activity Borda, E. Genaro, A. Peredo, H. Cremaschi, G. Leiros, C.P. Sterin-Borda, L. acetylsalicylic acid arachidonic acid indometacin prostaglandin prostaglandin e2 animal cell cytology drug administration drug mechanism drug monitoring heart heart atrium heart muscle contractility immunization macrophage mouse nonhuman preliminary communication priority journal thymocyte thymus Animals Arachidonic Acid Arachidonic Acids Immunization Macrophages Male Mice Mice, Inbred BALB C Mice, Inbred C3H Myocardial Contraction Prostaglandins E T-Lymphocytes The effect of BALB/c anti C3H non-adherent thymocytes on the spontaneous activity of isolated C3H mouse atria was studied. Immune non-adherent thymocytes alone induced negative inotropic action, while macrophages alone did not have any effect. The same kind of collaborative effect between both cells is described. Cell-free supernatants obtained from non-adherent immune cells incubated with C3H myocardium induced the same biological activity than immune non-adherent thymocytes indicating that a soluble factor is involved. Inhibition of non-adherent thymocytes' cyclo-oxygenase activity prevented the negative inotropic effect of immune cells or cell-free supernatants. In contrast, when the inhibitors were applied upon myocardium did not modify the response. Supernatants from immune thymocytes plus myocardium, release higher amount of PGE series than those from non-immune thymocytes. It is proposed that non-adherent thymocytes are able to synthesize and release PGE series upon recognition of alloantigens expressed by atria cells; which in turn triggers the negative inotropic effect. © 1985. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01920561_v7_n5_p647_Borda
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic acetylsalicylic acid
arachidonic acid
indometacin
prostaglandin
prostaglandin e2
animal cell
cytology
drug administration
drug mechanism
drug monitoring
heart
heart atrium
heart muscle contractility
immunization
macrophage
mouse
nonhuman
preliminary communication
priority journal
thymocyte
thymus
Animals
Arachidonic Acid
Arachidonic Acids
Immunization
Macrophages
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Myocardial Contraction
Prostaglandins E
T-Lymphocytes
spellingShingle acetylsalicylic acid
arachidonic acid
indometacin
prostaglandin
prostaglandin e2
animal cell
cytology
drug administration
drug mechanism
drug monitoring
heart
heart atrium
heart muscle contractility
immunization
macrophage
mouse
nonhuman
preliminary communication
priority journal
thymocyte
thymus
Animals
Arachidonic Acid
Arachidonic Acids
Immunization
Macrophages
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Myocardial Contraction
Prostaglandins E
T-Lymphocytes
Borda, E.
Genaro, A.
Peredo, H.
Cremaschi, G.
Leiros, C.P.
Sterin-Borda, L.
Alloimmunization-induced changes in thymocytes' prostaglandin levels: A signal for the induction of biological activity
topic_facet acetylsalicylic acid
arachidonic acid
indometacin
prostaglandin
prostaglandin e2
animal cell
cytology
drug administration
drug mechanism
drug monitoring
heart
heart atrium
heart muscle contractility
immunization
macrophage
mouse
nonhuman
preliminary communication
priority journal
thymocyte
thymus
Animals
Arachidonic Acid
Arachidonic Acids
Immunization
Macrophages
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Myocardial Contraction
Prostaglandins E
T-Lymphocytes
description The effect of BALB/c anti C3H non-adherent thymocytes on the spontaneous activity of isolated C3H mouse atria was studied. Immune non-adherent thymocytes alone induced negative inotropic action, while macrophages alone did not have any effect. The same kind of collaborative effect between both cells is described. Cell-free supernatants obtained from non-adherent immune cells incubated with C3H myocardium induced the same biological activity than immune non-adherent thymocytes indicating that a soluble factor is involved. Inhibition of non-adherent thymocytes' cyclo-oxygenase activity prevented the negative inotropic effect of immune cells or cell-free supernatants. In contrast, when the inhibitors were applied upon myocardium did not modify the response. Supernatants from immune thymocytes plus myocardium, release higher amount of PGE series than those from non-immune thymocytes. It is proposed that non-adherent thymocytes are able to synthesize and release PGE series upon recognition of alloantigens expressed by atria cells; which in turn triggers the negative inotropic effect. © 1985.
format JOUR
author Borda, E.
Genaro, A.
Peredo, H.
Cremaschi, G.
Leiros, C.P.
Sterin-Borda, L.
author_facet Borda, E.
Genaro, A.
Peredo, H.
Cremaschi, G.
Leiros, C.P.
Sterin-Borda, L.
author_sort Borda, E.
title Alloimmunization-induced changes in thymocytes' prostaglandin levels: A signal for the induction of biological activity
title_short Alloimmunization-induced changes in thymocytes' prostaglandin levels: A signal for the induction of biological activity
title_full Alloimmunization-induced changes in thymocytes' prostaglandin levels: A signal for the induction of biological activity
title_fullStr Alloimmunization-induced changes in thymocytes' prostaglandin levels: A signal for the induction of biological activity
title_full_unstemmed Alloimmunization-induced changes in thymocytes' prostaglandin levels: A signal for the induction of biological activity
title_sort alloimmunization-induced changes in thymocytes' prostaglandin levels: a signal for the induction of biological activity
url http://hdl.handle.net/20.500.12110/paper_01920561_v7_n5_p647_Borda
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AT genaroa alloimmunizationinducedchangesinthymocytesprostaglandinlevelsasignalfortheinductionofbiologicalactivity
AT peredoh alloimmunizationinducedchangesinthymocytesprostaglandinlevelsasignalfortheinductionofbiologicalactivity
AT cremaschig alloimmunizationinducedchangesinthymocytesprostaglandinlevelsasignalfortheinductionofbiologicalactivity
AT leiroscp alloimmunizationinducedchangesinthymocytesprostaglandinlevelsasignalfortheinductionofbiologicalactivity
AT sterinbordal alloimmunizationinducedchangesinthymocytesprostaglandinlevelsasignalfortheinductionofbiologicalactivity
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