Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina
A total of 89 freshly harvested soybean seed samples (Roundup Ready [transgenic] soybean cultivars) from the 2010/2011 crop season were collected from five locations in the Northern Pampean Region II, Argentina. These samples were analyzed for internal mycoflora, toxin production of isolated fungi,...
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todo:paper_01787888_v29_n3_p147_Garrido2023-10-03T15:08:26Z Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina Garrido, C.E. González, H.H.L. Salas, M.P. Resnik, S.L. Pacin, A.M. Alternaria Aspergillus Fusarium mycoflora mycotoxins Penicillium Soybean aflatoxin alternariol alternariol methyl ether fumonisin mycotoxin ochratoxin trichothecene derivative unclassified drug zearalenone Acremonium Alternaria Argentina Arthrinium phaeospermum article Aspergillus Chaetomium Chaetomium spinosum Cladosporium cladosporioides crop cultivar electron capture detection epicoccum nigrum Eurotium chevalieri fluorescence analysis food analysis food contamination fungal colonization fungal contamination fungus culture fungus isolation Fusarium gas chromatography high performance liquid chromatography limit of detection limit of quantitation Mucor mucor racemosus nonhuman Penicillium plant seed Rhizopus stolonifer Sclerotinia sclerotiorum soybean Thanatephorus cucumeris toxin analysis ulocladium Argentina Chromatography, Gas Chromatography, High Pressure Liquid Food Contamination Fungi Microbiological Techniques Mycotoxins Seeds Soybeans Alternaria Alternaria alternata Aspergillus Aspergillus flavus Aspergillus parasiticus Chaetomium Cladosporium Fungi Fusarium Gibberella moniliformis Gibberella zeae Glycine max Penicillium Penicillium citrinum Phomopsis Sclerotinia A total of 89 freshly harvested soybean seed samples (Roundup Ready [transgenic] soybean cultivars) from the 2010/2011 crop season were collected from five locations in the Northern Pampean Region II, Argentina. These samples were analyzed for internal mycoflora, toxin production of isolated fungi, and for a range of mycotoxins. Mycotoxin analysis of aflatoxins (AFs), zearalenone (ZEA), fumonisins (FBs) and ochratoxin A (OTA) was done by HPLC-FLD (high performance liquid chromatography with postcolumn fluorescence derivatization), alternariol and alternariol monomethyl ether with HPLC-UV (HPLC with UV detection), trichothecenes (deoxynivalenol, nivalenol, T-2 toxin, HT-2 toxin, fusarenon X, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol were analyzed by GC-ECD (gas chromatography with electron capture detector). Fungal colonization was more frequently found for samples from América, Saladillo and Trenque Lauquen than for samples from General Villegas and Trenel; a total of 1,401 fungal isolates were obtained from the soybean seeds. The most commonly identified fungal genera were Alternaria, Sclerotinia, Chaetomium, Cladosporium, Aspergillus, Penicillium, Phomopsis and Fusarium. Alternaria alternata, A.tenuissima, Aspergillus flavus, Penicillium citrinum, Fusarium verticillioides and F.semitectum were the predominant toxigenic fungal species. Mycotoxin production was confirmed for several isolates of toxigenic species, including Aspergillus flavus, A. parasiticus, Alternaria alternata, A.tenuissima, Fusarium graminearum, F semitectum and F. verticillioides. In particular, the percentage of mycotoxigenic Alternaria alternata (100 %), A.tenuissima (95 %) and aflatoxigenic strains of A. flavus (57 %) were remarkably high. Although none of the mycotoxins, AFs, ZEA, FBs, trichothecenes and OTA, were directly detected in samples of soybean seeds, the frequent presence of toxigenic fungal species indicates the risk of multiple mycotoxin contamination. © 2013 Society for Mycotoxin Research and Springer-Verlag Berlin Heidelberg. Fil:González, H.H.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Resnik, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01787888_v29_n3_p147_Garrido |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alternaria Aspergillus Fusarium mycoflora mycotoxins Penicillium Soybean aflatoxin alternariol alternariol methyl ether fumonisin mycotoxin ochratoxin trichothecene derivative unclassified drug zearalenone Acremonium Alternaria Argentina Arthrinium phaeospermum article Aspergillus Chaetomium Chaetomium spinosum Cladosporium cladosporioides crop cultivar electron capture detection epicoccum nigrum Eurotium chevalieri fluorescence analysis food analysis food contamination fungal colonization fungal contamination fungus culture fungus isolation Fusarium gas chromatography high performance liquid chromatography limit of detection limit of quantitation Mucor mucor racemosus nonhuman Penicillium plant seed Rhizopus stolonifer Sclerotinia sclerotiorum soybean Thanatephorus cucumeris toxin analysis ulocladium Argentina Chromatography, Gas Chromatography, High Pressure Liquid Food Contamination Fungi Microbiological Techniques Mycotoxins Seeds Soybeans Alternaria Alternaria alternata Aspergillus Aspergillus flavus Aspergillus parasiticus Chaetomium Cladosporium Fungi Fusarium Gibberella moniliformis Gibberella zeae Glycine max Penicillium Penicillium citrinum Phomopsis Sclerotinia |
| spellingShingle |
Alternaria Aspergillus Fusarium mycoflora mycotoxins Penicillium Soybean aflatoxin alternariol alternariol methyl ether fumonisin mycotoxin ochratoxin trichothecene derivative unclassified drug zearalenone Acremonium Alternaria Argentina Arthrinium phaeospermum article Aspergillus Chaetomium Chaetomium spinosum Cladosporium cladosporioides crop cultivar electron capture detection epicoccum nigrum Eurotium chevalieri fluorescence analysis food analysis food contamination fungal colonization fungal contamination fungus culture fungus isolation Fusarium gas chromatography high performance liquid chromatography limit of detection limit of quantitation Mucor mucor racemosus nonhuman Penicillium plant seed Rhizopus stolonifer Sclerotinia sclerotiorum soybean Thanatephorus cucumeris toxin analysis ulocladium Argentina Chromatography, Gas Chromatography, High Pressure Liquid Food Contamination Fungi Microbiological Techniques Mycotoxins Seeds Soybeans Alternaria Alternaria alternata Aspergillus Aspergillus flavus Aspergillus parasiticus Chaetomium Cladosporium Fungi Fusarium Gibberella moniliformis Gibberella zeae Glycine max Penicillium Penicillium citrinum Phomopsis Sclerotinia Garrido, C.E. González, H.H.L. Salas, M.P. Resnik, S.L. Pacin, A.M. Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina |
| topic_facet |
Alternaria Aspergillus Fusarium mycoflora mycotoxins Penicillium Soybean aflatoxin alternariol alternariol methyl ether fumonisin mycotoxin ochratoxin trichothecene derivative unclassified drug zearalenone Acremonium Alternaria Argentina Arthrinium phaeospermum article Aspergillus Chaetomium Chaetomium spinosum Cladosporium cladosporioides crop cultivar electron capture detection epicoccum nigrum Eurotium chevalieri fluorescence analysis food analysis food contamination fungal colonization fungal contamination fungus culture fungus isolation Fusarium gas chromatography high performance liquid chromatography limit of detection limit of quantitation Mucor mucor racemosus nonhuman Penicillium plant seed Rhizopus stolonifer Sclerotinia sclerotiorum soybean Thanatephorus cucumeris toxin analysis ulocladium Argentina Chromatography, Gas Chromatography, High Pressure Liquid Food Contamination Fungi Microbiological Techniques Mycotoxins Seeds Soybeans Alternaria Alternaria alternata Aspergillus Aspergillus flavus Aspergillus parasiticus Chaetomium Cladosporium Fungi Fusarium Gibberella moniliformis Gibberella zeae Glycine max Penicillium Penicillium citrinum Phomopsis Sclerotinia |
| description |
A total of 89 freshly harvested soybean seed samples (Roundup Ready [transgenic] soybean cultivars) from the 2010/2011 crop season were collected from five locations in the Northern Pampean Region II, Argentina. These samples were analyzed for internal mycoflora, toxin production of isolated fungi, and for a range of mycotoxins. Mycotoxin analysis of aflatoxins (AFs), zearalenone (ZEA), fumonisins (FBs) and ochratoxin A (OTA) was done by HPLC-FLD (high performance liquid chromatography with postcolumn fluorescence derivatization), alternariol and alternariol monomethyl ether with HPLC-UV (HPLC with UV detection), trichothecenes (deoxynivalenol, nivalenol, T-2 toxin, HT-2 toxin, fusarenon X, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol were analyzed by GC-ECD (gas chromatography with electron capture detector). Fungal colonization was more frequently found for samples from América, Saladillo and Trenque Lauquen than for samples from General Villegas and Trenel; a total of 1,401 fungal isolates were obtained from the soybean seeds. The most commonly identified fungal genera were Alternaria, Sclerotinia, Chaetomium, Cladosporium, Aspergillus, Penicillium, Phomopsis and Fusarium. Alternaria alternata, A.tenuissima, Aspergillus flavus, Penicillium citrinum, Fusarium verticillioides and F.semitectum were the predominant toxigenic fungal species. Mycotoxin production was confirmed for several isolates of toxigenic species, including Aspergillus flavus, A. parasiticus, Alternaria alternata, A.tenuissima, Fusarium graminearum, F semitectum and F. verticillioides. In particular, the percentage of mycotoxigenic Alternaria alternata (100 %), A.tenuissima (95 %) and aflatoxigenic strains of A. flavus (57 %) were remarkably high. Although none of the mycotoxins, AFs, ZEA, FBs, trichothecenes and OTA, were directly detected in samples of soybean seeds, the frequent presence of toxigenic fungal species indicates the risk of multiple mycotoxin contamination. © 2013 Society for Mycotoxin Research and Springer-Verlag Berlin Heidelberg. |
| format |
JOUR |
| author |
Garrido, C.E. González, H.H.L. Salas, M.P. Resnik, S.L. Pacin, A.M. |
| author_facet |
Garrido, C.E. González, H.H.L. Salas, M.P. Resnik, S.L. Pacin, A.M. |
| author_sort |
Garrido, C.E. |
| title |
Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina |
| title_short |
Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina |
| title_full |
Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina |
| title_fullStr |
Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina |
| title_full_unstemmed |
Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina |
| title_sort |
mycoflora and mycotoxin contamination of roundup ready soybean harvested in the pampean region, argentina |
| url |
http://hdl.handle.net/20.500.12110/paper_01787888_v29_n3_p147_Garrido |
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