Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture
Plant regeneration was induced with 40 and 400-day-old leaf explant derived callus from tetraploid (2n = 24) Tradescantia crassifoilia Cav. Schenk & Hildebrandt medium supplemented with 0.8 mg · 1−1 2,4-D + 0.1 mg · 1−1 Kin was the most suitable combination for callus induction. Shoot regene...
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| Autores principales: | , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01761617_v119_n5_p467_Escandon |
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| Sumario: | Plant regeneration was induced with 40 and 400-day-old leaf explant derived callus from tetraploid (2n = 24) Tradescantia crassifoilia Cav. Schenk & Hildebrandt medium supplemented with 0.8 mg · 1−1 2,4-D + 0.1 mg · 1−1 Kin was the most suitable combination for callus induction. Shoot regeneration was achieved in the same medium, but with either 0.5 to 2.0 mg · 1−1 BAP or with a combination of 0.01 mg · 1−1 of NAA plus from 0.1 to 5.0 mg - 1−1 of BAP. The chromosome number both in the calli and in the roots differentiated from them in the 40-day-old calli (2 subcultures) was 2n = 24. However in the 400-day-old tissue (20 subcultures) only chromosome counts of 2n = 21 were obtained in the calli and in the differentiated roots. Also 2n = 20, 21 and 42 were counted in 17 regenerants from 400-day-old calli. The most frequent chromosome number in the latter (15 individuals) was 2n = 21. © 1985, Gustav Fischer Verlag, Stuttgart. All rights reserved. |
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