Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture

Plant regeneration was induced with 40 and 400-day-old leaf explant derived callus from tetraploid (2n = 24) Tradescantia crassifoilia Cav. Schenk & Hildebrandt medium supplemented with 0.8 mg · 1−1 2,4-D + 0.1 mg · 1−1 Kin was the most suitable combination for callus induction. Shoot regene...

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Autores principales:	Escandón, A., Martínez, A., Caso, O.H., Pomar, M.C.
Formato:	JOUR
Materias:	2,4-D 2,4-Dichlorophenoxyacetic acid 6-benzylaminopurine 6-fufuryl aminopurine alphanaphthaleneacetic acid aneuploid plants BAP callus culture Kin NAA regenerants Tradescantia crassifolia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01761617_v119_n5_p467_Escandon
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01761617_v119_n5_p467_Escandon
record_format	dspace
spelling	todo:paper_01761617_v119_n5_p467_Escandon2023-10-03T15:08:01Z Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture Escandón, A. Martínez, A. Caso, O.H. Pomar, M.C. 2,4-D 2,4-Dichlorophenoxyacetic acid 6-benzylaminopurine 6-fufuryl aminopurine alphanaphthaleneacetic acid aneuploid plants BAP callus culture Kin NAA regenerants Tradescantia crassifolia Plant regeneration was induced with 40 and 400-day-old leaf explant derived callus from tetraploid (2n = 24) Tradescantia crassifoilia Cav. Schenk & Hildebrandt medium supplemented with 0.8 mg · 1−1 2,4-D + 0.1 mg · 1−1 Kin was the most suitable combination for callus induction. Shoot regeneration was achieved in the same medium, but with either 0.5 to 2.0 mg · 1−1 BAP or with a combination of 0.01 mg · 1−1 of NAA plus from 0.1 to 5.0 mg - 1−1 of BAP. The chromosome number both in the calli and in the roots differentiated from them in the 40-day-old calli (2 subcultures) was 2n = 24. However in the 400-day-old tissue (20 subcultures) only chromosome counts of 2n = 21 were obtained in the calli and in the differentiated roots. Also 2n = 20, 21 and 42 were counted in 17 regenerants from 400-day-old calli. The most frequent chromosome number in the latter (15 individuals) was 2n = 21. © 1985, Gustav Fischer Verlag, Stuttgart. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01761617_v119_n5_p467_Escandon
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	2,4-D 2,4-Dichlorophenoxyacetic acid 6-benzylaminopurine 6-fufuryl aminopurine alphanaphthaleneacetic acid aneuploid plants BAP callus culture Kin NAA regenerants Tradescantia crassifolia
spellingShingle	2,4-D 2,4-Dichlorophenoxyacetic acid 6-benzylaminopurine 6-fufuryl aminopurine alphanaphthaleneacetic acid aneuploid plants BAP callus culture Kin NAA regenerants Tradescantia crassifolia Escandón, A. Martínez, A. Caso, O.H. Pomar, M.C. Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture
topic_facet	2,4-D 2,4-Dichlorophenoxyacetic acid 6-benzylaminopurine 6-fufuryl aminopurine alphanaphthaleneacetic acid aneuploid plants BAP callus culture Kin NAA regenerants Tradescantia crassifolia
description	Plant regeneration was induced with 40 and 400-day-old leaf explant derived callus from tetraploid (2n = 24) Tradescantia crassifoilia Cav. Schenk & Hildebrandt medium supplemented with 0.8 mg · 1−1 2,4-D + 0.1 mg · 1−1 Kin was the most suitable combination for callus induction. Shoot regeneration was achieved in the same medium, but with either 0.5 to 2.0 mg · 1−1 BAP or with a combination of 0.01 mg · 1−1 of NAA plus from 0.1 to 5.0 mg - 1−1 of BAP. The chromosome number both in the calli and in the roots differentiated from them in the 40-day-old calli (2 subcultures) was 2n = 24. However in the 400-day-old tissue (20 subcultures) only chromosome counts of 2n = 21 were obtained in the calli and in the differentiated roots. Also 2n = 20, 21 and 42 were counted in 17 regenerants from 400-day-old calli. The most frequent chromosome number in the latter (15 individuals) was 2n = 21. © 1985, Gustav Fischer Verlag, Stuttgart. All rights reserved.
format	JOUR
author	Escandón, A. Martínez, A. Caso, O.H. Pomar, M.C.
author_facet	Escandón, A. Martínez, A. Caso, O.H. Pomar, M.C.
author_sort	Escandón, A.
title	Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture
title_short	Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture
title_full	Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture
title_fullStr	Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture
title_full_unstemmed	Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture
title_sort	regeneration of aneuploid plants from tetraploid (2n = 24) tradescantia crassifolia cav. through callus culture
url	http://hdl.handle.net/20.500.12110/paper_01761617_v119_n5_p467_Escandon
work_keys_str_mv	AT escandona regenerationofaneuploidplantsfromtetraploid2n24tradescantiacrassifoliacavthroughcallusculture AT martineza regenerationofaneuploidplantsfromtetraploid2n24tradescantiacrassifoliacavthroughcallusculture AT casooh regenerationofaneuploidplantsfromtetraploid2n24tradescantiacrassifoliacavthroughcallusculture AT pomarmc regenerationofaneuploidplantsfromtetraploid2n24tradescantiacrassifoliacavthroughcallusculture
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Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture

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