Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus
The present study employs an in vitro system to analyse the role of steroid hormones in hCG-induced spermiation in two species of anuran amphibian: Rana catesbeiana and Leptodactylus ocellatus. In vitro spermiation was induced with 10 IU hCG and the effect of different steroid-biosynthesis inhibitor...
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todo:paper_01741578_v176_n6_p559_Rosemblit2023-10-03T15:07:48Z Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus Rosemblit, C. Pozzi, A.G. Ceballos, N.R. Amphibians Anura hCG Spermiation Steroids 17,20 dihydroxy 4 pregnen 3 one 17,20-dihydroxy-4-pregnen-3-one androgen androstanolone chorionic gonadotropin progesterone progesterone derivative steroid testosterone animal article biosynthesis frog frogs and toads human male physiology secretion spermatogenesis Androgens Animals Anura Chorionic Gonadotropin Dihydrotestosterone Humans Hydroxyprogesterones Male Progesterone Rana catesbeiana Spermatogenesis Steroids Testosterone Amphibia Anura Bufo arenarum Leptodactylus ocellatus Rana catesbeiana The present study employs an in vitro system to analyse the role of steroid hormones in hCG-induced spermiation in two species of anuran amphibian: Rana catesbeiana and Leptodactylus ocellatus. In vitro spermiation was induced with 10 IU hCG and the effect of different steroid-biosynthesis inhibitors was analysed. Cyanoketone (10-5 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of androgen was significantly reduced. These results clearly showed that, in both species, spermiation-inducing action of hCG does not depend on the biosynthesis of 3-oxo-4-ene steroids. Moreover, when combined inhibitors, aminoglutethimide (10-5 M) plus cyanoketone (10-5 M), were employed, spermiation evoked by hCG was not modified while hCG-induced androgen secretion significantly decreased. Additionally, none of the steroids used, progesterone, 17, 20α-dihydroxy-4-pregnen-3-one, testosterone and 5α- dihydrotestosterone, were able to induce spermiation in the absence of hCG, confirming that steroids are not involved in that process. In conclusion, as previously described in Bufo arenarum, in L. ocellatus and R. catesbeiana hCG-induced spermiation does not depend on steroid biosynthesis. © Springer-Verlag 2006. Fil:Rosemblit, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01741578_v176_n6_p559_Rosemblit |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amphibians Anura hCG Spermiation Steroids 17,20 dihydroxy 4 pregnen 3 one 17,20-dihydroxy-4-pregnen-3-one androgen androstanolone chorionic gonadotropin progesterone progesterone derivative steroid testosterone animal article biosynthesis frog frogs and toads human male physiology secretion spermatogenesis Androgens Animals Anura Chorionic Gonadotropin Dihydrotestosterone Humans Hydroxyprogesterones Male Progesterone Rana catesbeiana Spermatogenesis Steroids Testosterone Amphibia Anura Bufo arenarum Leptodactylus ocellatus Rana catesbeiana |
| spellingShingle |
Amphibians Anura hCG Spermiation Steroids 17,20 dihydroxy 4 pregnen 3 one 17,20-dihydroxy-4-pregnen-3-one androgen androstanolone chorionic gonadotropin progesterone progesterone derivative steroid testosterone animal article biosynthesis frog frogs and toads human male physiology secretion spermatogenesis Androgens Animals Anura Chorionic Gonadotropin Dihydrotestosterone Humans Hydroxyprogesterones Male Progesterone Rana catesbeiana Spermatogenesis Steroids Testosterone Amphibia Anura Bufo arenarum Leptodactylus ocellatus Rana catesbeiana Rosemblit, C. Pozzi, A.G. Ceballos, N.R. Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus |
| topic_facet |
Amphibians Anura hCG Spermiation Steroids 17,20 dihydroxy 4 pregnen 3 one 17,20-dihydroxy-4-pregnen-3-one androgen androstanolone chorionic gonadotropin progesterone progesterone derivative steroid testosterone animal article biosynthesis frog frogs and toads human male physiology secretion spermatogenesis Androgens Animals Anura Chorionic Gonadotropin Dihydrotestosterone Humans Hydroxyprogesterones Male Progesterone Rana catesbeiana Spermatogenesis Steroids Testosterone Amphibia Anura Bufo arenarum Leptodactylus ocellatus Rana catesbeiana |
| description |
The present study employs an in vitro system to analyse the role of steroid hormones in hCG-induced spermiation in two species of anuran amphibian: Rana catesbeiana and Leptodactylus ocellatus. In vitro spermiation was induced with 10 IU hCG and the effect of different steroid-biosynthesis inhibitors was analysed. Cyanoketone (10-5 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of androgen was significantly reduced. These results clearly showed that, in both species, spermiation-inducing action of hCG does not depend on the biosynthesis of 3-oxo-4-ene steroids. Moreover, when combined inhibitors, aminoglutethimide (10-5 M) plus cyanoketone (10-5 M), were employed, spermiation evoked by hCG was not modified while hCG-induced androgen secretion significantly decreased. Additionally, none of the steroids used, progesterone, 17, 20α-dihydroxy-4-pregnen-3-one, testosterone and 5α- dihydrotestosterone, were able to induce spermiation in the absence of hCG, confirming that steroids are not involved in that process. In conclusion, as previously described in Bufo arenarum, in L. ocellatus and R. catesbeiana hCG-induced spermiation does not depend on steroid biosynthesis. © Springer-Verlag 2006. |
| format |
JOUR |
| author |
Rosemblit, C. Pozzi, A.G. Ceballos, N.R. |
| author_facet |
Rosemblit, C. Pozzi, A.G. Ceballos, N.R. |
| author_sort |
Rosemblit, C. |
| title |
Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus |
| title_short |
Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus |
| title_full |
Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus |
| title_fullStr |
Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus |
| title_full_unstemmed |
Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus |
| title_sort |
relationship between steroidogenesis and spermiation in rana catesbeiana and leptodactylus ocellatus |
| url |
http://hdl.handle.net/20.500.12110/paper_01741578_v176_n6_p559_Rosemblit |
| work_keys_str_mv |
AT rosemblitc relationshipbetweensteroidogenesisandspermiationinranacatesbeianaandleptodactylusocellatus AT pozziag relationshipbetweensteroidogenesisandspermiationinranacatesbeianaandleptodactylusocellatus AT ceballosnr relationshipbetweensteroidogenesisandspermiationinranacatesbeianaandleptodactylusocellatus |
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1782025780342030336 |