Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel

(125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, whi...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lüthy, I.A., Segura, E.T., Lüthy, V.I., Charreau, E.H., Calandra, R.S.
Formato: JOUR
Materias:
hormone receptor
prolactin i 125
radioisotope
animal cell
central nervous system
drug receptor binding
kidney
nonhuman
pharmacokinetics
priority journal
Animals
Binding, Competitive
Brain
Bufo arenarum
Kidney
Kinetics
Male
Prolactin
Receptors, Cell Surface
Receptors, Prolactin
Sheep
Tissue Distribution
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01741578_v155_n5_p611_Luthy
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01741578_v155_n5_p611_Luthy
record_format dspace
spelling todo:paper_01741578_v155_n5_p611_Luthy2023-10-03T15:07:45Z Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel Lüthy, I.A. Segura, E.T. Lüthy, V.I. Charreau, E.H. Calandra, R.S. hormone receptor prolactin i 125 radioisotope animal cell central nervous system drug receptor binding kidney nonhuman pharmacokinetics priority journal Animals Binding, Competitive Brain Bufo arenarum Kidney Kinetics Male Prolactin Receptors, Cell Surface Receptors, Prolactin Sheep Tissue Distribution (125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, while the association constant (Ka) by Scatchard analysis was between 4.0 to 8.7×109 M-1. This binding was compared with the Scatchard plot of the kidney, which has been already described by other groups, and gave 41.7 fmol/mg protein and Ka 2.5×109 M-1. Liver showed no binding and in the cerebral hemispheres (125I)-oPRL was not displaced by non-lactogenic hormones, indicating that binding was hormone and tissue specific. © 1985 Springer-Verlag. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01741578_v155_n5_p611_Luthy
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic hormone receptor
prolactin i 125
radioisotope
animal cell
central nervous system
drug receptor binding
kidney
nonhuman
pharmacokinetics
priority journal
Animals
Binding, Competitive
Brain
Bufo arenarum
Kidney
Kinetics
Male
Prolactin
Receptors, Cell Surface
Receptors, Prolactin
Sheep
Tissue Distribution
spellingShingle hormone receptor
prolactin i 125
radioisotope
animal cell
central nervous system
drug receptor binding
kidney
nonhuman
pharmacokinetics
priority journal
Animals
Binding, Competitive
Brain
Bufo arenarum
Kidney
Kinetics
Male
Prolactin
Receptors, Cell Surface
Receptors, Prolactin
Sheep
Tissue Distribution
Lüthy, I.A.
Segura, E.T.
Lüthy, V.I.
Charreau, E.H.
Calandra, R.S.
Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel
topic_facet hormone receptor
prolactin i 125
radioisotope
animal cell
central nervous system
drug receptor binding
kidney
nonhuman
pharmacokinetics
priority journal
Animals
Binding, Competitive
Brain
Bufo arenarum
Kidney
Kinetics
Male
Prolactin
Receptors, Cell Surface
Receptors, Prolactin
Sheep
Tissue Distribution
description (125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, while the association constant (Ka) by Scatchard analysis was between 4.0 to 8.7×109 M-1. This binding was compared with the Scatchard plot of the kidney, which has been already described by other groups, and gave 41.7 fmol/mg protein and Ka 2.5×109 M-1. Liver showed no binding and in the cerebral hemispheres (125I)-oPRL was not displaced by non-lactogenic hormones, indicating that binding was hormone and tissue specific. © 1985 Springer-Verlag.
format JOUR
author Lüthy, I.A.
Segura, E.T.
Lüthy, V.I.
Charreau, E.H.
Calandra, R.S.
author_facet Lüthy, I.A.
Segura, E.T.
Lüthy, V.I.
Charreau, E.H.
Calandra, R.S.
author_sort Lüthy, I.A.
title Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel
title_short Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel
title_full Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel
title_fullStr Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel
title_full_unstemmed Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel
title_sort prolactin binding sites in the brain and kidneys of the toad, bufo arenarum hensel
url http://hdl.handle.net/20.500.12110/paper_01741578_v155_n5_p611_Luthy
work_keys_str_mv AT luthyia prolactinbindingsitesinthebrainandkidneysofthetoadbufoarenarumhensel
AT seguraet prolactinbindingsitesinthebrainandkidneysofthetoadbufoarenarumhensel
AT luthyvi prolactinbindingsitesinthebrainandkidneysofthetoadbufoarenarumhensel
AT charreaueh prolactinbindingsitesinthebrainandkidneysofthetoadbufoarenarumhensel
AT calandrars prolactinbindingsitesinthebrainandkidneysofthetoadbufoarenarumhensel
_version_ 1807315013652709376

Ejemplares similares