Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats
OBJECTIVES: The aim of present paper was to study the probable role of glutamic acid (GLU) as a mediator of bisphenol A (BPA) effect at the hypothalamic level and its effects on the reproductive axis of prepubertal male rats. METHODS: Mated Wistar rats were treated with either 0.1% ethanol (control...
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todo:paper_0172780X_v31_n4_p512_Cardoso2023-10-03T15:07:38Z Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats Cardoso, N. Pandolfi, M. Ponzo, O. Carbone, S. Szwarcfarb, B. Scacchi, P. Reynoso, R. Bisphenol A FSH Glutamic acid Gn-RH LH Prepubertal 4,4' isopropylidenediphenol follitropin glutamic acid gonadorelin luteinizing hormone testosterone animal experiment animal tissue article controlled study female hypothalamus male nonhuman prepuberty rat Animals Chromatography, High Pressure Liquid Female Follicle Stimulating Hormone Glutamic Acid Gonadotropin-Releasing Hormone Hypothalamus Luteinizing Hormone Male Phenols Pregnancy Prenatal Exposure Delayed Effects Radioimmunoassay Rats Rats, Wistar Sexual Maturation Testosterone OBJECTIVES: The aim of present paper was to study the probable role of glutamic acid (GLU) as a mediator of bisphenol A (BPA) effect at the hypothalamic level and its effects on the reproductive axis of prepubertal male rats. METHODS: Mated Wistar rats were treated with either 0.1% ethanol (control group, n=10) or BPA (BPA group, n=10) in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight/day of BPA. At the prepubertal stage (35 days of age), the male rats were sacrificed and Gn-RH and glutamic acid (GLU) release, an amino acid involved in Gn-RH secretion, were measured in hypothalamic samples containing medio basal and anterior preoptic area (MBH-APOA), by RIA and HPLC respectively. LH, FSH serum levels were measured by RIA and testosterone by EQLIA. RESULTS: Gn-RH and GLU release decreased significantly in animals exposed to BPA (p<0.001, p<0.01). LH, FSH and testosterone serum levels were also decreased by treatment (p<0.0001). CONCLUSION: Present results provide evidence that BPA may act at the hypothalamic level to decrease GLU release which in turn may modify Gn-RH secretion altering the normal function of the axis. © 2010 Neuroendocrinology Letters. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0172780X_v31_n4_p512_Cardoso |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bisphenol A FSH Glutamic acid Gn-RH LH Prepubertal 4,4' isopropylidenediphenol follitropin glutamic acid gonadorelin luteinizing hormone testosterone animal experiment animal tissue article controlled study female hypothalamus male nonhuman prepuberty rat Animals Chromatography, High Pressure Liquid Female Follicle Stimulating Hormone Glutamic Acid Gonadotropin-Releasing Hormone Hypothalamus Luteinizing Hormone Male Phenols Pregnancy Prenatal Exposure Delayed Effects Radioimmunoassay Rats Rats, Wistar Sexual Maturation Testosterone |
| spellingShingle |
Bisphenol A FSH Glutamic acid Gn-RH LH Prepubertal 4,4' isopropylidenediphenol follitropin glutamic acid gonadorelin luteinizing hormone testosterone animal experiment animal tissue article controlled study female hypothalamus male nonhuman prepuberty rat Animals Chromatography, High Pressure Liquid Female Follicle Stimulating Hormone Glutamic Acid Gonadotropin-Releasing Hormone Hypothalamus Luteinizing Hormone Male Phenols Pregnancy Prenatal Exposure Delayed Effects Radioimmunoassay Rats Rats, Wistar Sexual Maturation Testosterone Cardoso, N. Pandolfi, M. Ponzo, O. Carbone, S. Szwarcfarb, B. Scacchi, P. Reynoso, R. Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats |
| topic_facet |
Bisphenol A FSH Glutamic acid Gn-RH LH Prepubertal 4,4' isopropylidenediphenol follitropin glutamic acid gonadorelin luteinizing hormone testosterone animal experiment animal tissue article controlled study female hypothalamus male nonhuman prepuberty rat Animals Chromatography, High Pressure Liquid Female Follicle Stimulating Hormone Glutamic Acid Gonadotropin-Releasing Hormone Hypothalamus Luteinizing Hormone Male Phenols Pregnancy Prenatal Exposure Delayed Effects Radioimmunoassay Rats Rats, Wistar Sexual Maturation Testosterone |
| description |
OBJECTIVES: The aim of present paper was to study the probable role of glutamic acid (GLU) as a mediator of bisphenol A (BPA) effect at the hypothalamic level and its effects on the reproductive axis of prepubertal male rats. METHODS: Mated Wistar rats were treated with either 0.1% ethanol (control group, n=10) or BPA (BPA group, n=10) in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight/day of BPA. At the prepubertal stage (35 days of age), the male rats were sacrificed and Gn-RH and glutamic acid (GLU) release, an amino acid involved in Gn-RH secretion, were measured in hypothalamic samples containing medio basal and anterior preoptic area (MBH-APOA), by RIA and HPLC respectively. LH, FSH serum levels were measured by RIA and testosterone by EQLIA. RESULTS: Gn-RH and GLU release decreased significantly in animals exposed to BPA (p<0.001, p<0.01). LH, FSH and testosterone serum levels were also decreased by treatment (p<0.0001). CONCLUSION: Present results provide evidence that BPA may act at the hypothalamic level to decrease GLU release which in turn may modify Gn-RH secretion altering the normal function of the axis. © 2010 Neuroendocrinology Letters. |
| format |
JOUR |
| author |
Cardoso, N. Pandolfi, M. Ponzo, O. Carbone, S. Szwarcfarb, B. Scacchi, P. Reynoso, R. |
| author_facet |
Cardoso, N. Pandolfi, M. Ponzo, O. Carbone, S. Szwarcfarb, B. Scacchi, P. Reynoso, R. |
| author_sort |
Cardoso, N. |
| title |
Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats |
| title_short |
Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats |
| title_full |
Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats |
| title_fullStr |
Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats |
| title_full_unstemmed |
Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats |
| title_sort |
evidence to suggest glutamic acid involvement in bisphenol a effect at the hypothalamic level in prepubertal male rats |
| url |
http://hdl.handle.net/20.500.12110/paper_0172780X_v31_n4_p512_Cardoso |
| work_keys_str_mv |
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