Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions

Our aim in this work was to further characterize the complexity and specificity of the three different isoforms (Tpk1, Tpk2 and Tpk3) of the catalytic and regulatory (Bcy1) subunits of PKA in Saccharomyces cerevisiae. We thus analyzed the subcellular localization of the PKA subunits in living cells...

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Autores principales:	Tudisca, V., Recouvreux, V., Moreno, S., Boy-Marcotte, E., Jacquet, M., Portela, P.
Formato:	JOUR
Materias:	P-bodies PKA subunits Saccharomyces cerevisiae Stationary phase cyclic AMP dependent protein kinase fungal protein glucose glycerol green fluorescent protein protein Bcy1 protein Tpk1 protein Tpk2 protein Tpk3 unclassified drug culture medium isoenzyme protein subunit article carbon source cell nucleus cellular distribution controlled study cytoplasm fermentation fungal strain fungus growth gene deletion hyperosmotic stress kinetics nonhuman nuclear localization signal priority journal protein analysis protein expression cell granule chemistry cytology enzymology genetics growth, development and aging metabolism protein transport reproducibility Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01719335_v89_n4_p339_Tudisca
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01719335_v89_n4_p339_Tudisca2023-10-03T15:07:36Z Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions Tudisca, V. Recouvreux, V. Moreno, S. Boy-Marcotte, E. Jacquet, M. Portela, P. P-bodies PKA subunits Saccharomyces cerevisiae Stationary phase cyclic AMP dependent protein kinase fungal protein glucose glycerol green fluorescent protein protein Bcy1 protein Tpk1 protein Tpk2 protein Tpk3 unclassified drug culture medium cyclic AMP dependent protein kinase green fluorescent protein isoenzyme protein subunit article carbon source cell nucleus cellular distribution controlled study cytoplasm fermentation fungal strain fungus growth gene deletion hyperosmotic stress kinetics nonhuman nuclear localization signal priority journal protein analysis protein expression Saccharomyces cerevisiae cell granule chemistry culture medium cytology enzymology genetics growth, development and aging metabolism protein subunit protein transport reproducibility Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results Saccharomyces cerevisiae Saccharomyces cerevisiae Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results Saccharomyces cerevisiae Our aim in this work was to further characterize the complexity and specificity of the three different isoforms (Tpk1, Tpk2 and Tpk3) of the catalytic and regulatory (Bcy1) subunits of PKA in Saccharomyces cerevisiae. We thus analyzed the subcellular localization of the PKA subunits in living cells by using strains carrying GFP (green fluorescent protein) fused to each PKA subunit. During exponential growth on glucose, both Bcy1 and Tpk2 localized in the nucleus, whereas Tpk1 and Tpk3 showed a mixed pattern of nucleo-cytoplasmic localization. During exponential growth on glycerol and during stationary phase, the PKA subunits showed mostly cytoplasmic localization, with the peculiarity that Tpk2 and Tpk3 but not Bcy1, were found associated to P-bodies and EGP bodies. Tpk3 was accumulated into P-bodies during glucose deprivation and hyper osmotic stress. Deletion of Tpk3 altered the kinetics of P-body formation. Analysis of protein expression showed that the relative expression pattern of each Tpk changes from low levels under fermentative metabolism to higher levels during stationary phase. The increase in Tpk levels produced an imbalance with Bcy1 levels. Our data suggest that the signaling specificity through PKA in yeast could be mediated by a particular subcellular localization of each isoform of Tpk. © 2009 Elsevier GmbH. All rights reserved. Fil:Tudisca, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Recouvreux, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Portela, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01719335_v89_n4_p339_Tudisca
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	P-bodies PKA subunits Saccharomyces cerevisiae Stationary phase cyclic AMP dependent protein kinase fungal protein glucose glycerol green fluorescent protein protein Bcy1 protein Tpk1 protein Tpk2 protein Tpk3 unclassified drug culture medium cyclic AMP dependent protein kinase green fluorescent protein isoenzyme protein subunit article carbon source cell nucleus cellular distribution controlled study cytoplasm fermentation fungal strain fungus growth gene deletion hyperosmotic stress kinetics nonhuman nuclear localization signal priority journal protein analysis protein expression Saccharomyces cerevisiae cell granule chemistry culture medium cytology enzymology genetics growth, development and aging metabolism protein subunit protein transport reproducibility Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results Saccharomyces cerevisiae Saccharomyces cerevisiae Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results Saccharomyces cerevisiae
spellingShingle	P-bodies PKA subunits Saccharomyces cerevisiae Stationary phase cyclic AMP dependent protein kinase fungal protein glucose glycerol green fluorescent protein protein Bcy1 protein Tpk1 protein Tpk2 protein Tpk3 unclassified drug culture medium cyclic AMP dependent protein kinase green fluorescent protein isoenzyme protein subunit article carbon source cell nucleus cellular distribution controlled study cytoplasm fermentation fungal strain fungus growth gene deletion hyperosmotic stress kinetics nonhuman nuclear localization signal priority journal protein analysis protein expression Saccharomyces cerevisiae cell granule chemistry culture medium cytology enzymology genetics growth, development and aging metabolism protein subunit protein transport reproducibility Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results Saccharomyces cerevisiae Saccharomyces cerevisiae Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results Saccharomyces cerevisiae Tudisca, V. Recouvreux, V. Moreno, S. Boy-Marcotte, E. Jacquet, M. Portela, P. Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions
topic_facet	P-bodies PKA subunits Saccharomyces cerevisiae Stationary phase cyclic AMP dependent protein kinase fungal protein glucose glycerol green fluorescent protein protein Bcy1 protein Tpk1 protein Tpk2 protein Tpk3 unclassified drug culture medium cyclic AMP dependent protein kinase green fluorescent protein isoenzyme protein subunit article carbon source cell nucleus cellular distribution controlled study cytoplasm fermentation fungal strain fungus growth gene deletion hyperosmotic stress kinetics nonhuman nuclear localization signal priority journal protein analysis protein expression Saccharomyces cerevisiae cell granule chemistry culture medium cytology enzymology genetics growth, development and aging metabolism protein subunit protein transport reproducibility Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results Saccharomyces cerevisiae Saccharomyces cerevisiae Cell Nucleus Culture Media Cyclic AMP-Dependent Protein Kinases Cytoplasm Cytoplasmic Granules Green Fluorescent Proteins Isoenzymes Protein Subunits Protein Transport Reproducibility of Results Saccharomyces cerevisiae
description	Our aim in this work was to further characterize the complexity and specificity of the three different isoforms (Tpk1, Tpk2 and Tpk3) of the catalytic and regulatory (Bcy1) subunits of PKA in Saccharomyces cerevisiae. We thus analyzed the subcellular localization of the PKA subunits in living cells by using strains carrying GFP (green fluorescent protein) fused to each PKA subunit. During exponential growth on glucose, both Bcy1 and Tpk2 localized in the nucleus, whereas Tpk1 and Tpk3 showed a mixed pattern of nucleo-cytoplasmic localization. During exponential growth on glycerol and during stationary phase, the PKA subunits showed mostly cytoplasmic localization, with the peculiarity that Tpk2 and Tpk3 but not Bcy1, were found associated to P-bodies and EGP bodies. Tpk3 was accumulated into P-bodies during glucose deprivation and hyper osmotic stress. Deletion of Tpk3 altered the kinetics of P-body formation. Analysis of protein expression showed that the relative expression pattern of each Tpk changes from low levels under fermentative metabolism to higher levels during stationary phase. The increase in Tpk levels produced an imbalance with Bcy1 levels. Our data suggest that the signaling specificity through PKA in yeast could be mediated by a particular subcellular localization of each isoform of Tpk. © 2009 Elsevier GmbH. All rights reserved.
format	JOUR
author	Tudisca, V. Recouvreux, V. Moreno, S. Boy-Marcotte, E. Jacquet, M. Portela, P.
author_facet	Tudisca, V. Recouvreux, V. Moreno, S. Boy-Marcotte, E. Jacquet, M. Portela, P.
author_sort	Tudisca, V.
title	Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions
title_short	Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions
title_full	Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions
title_fullStr	Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions
title_full_unstemmed	Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions
title_sort	differential localization to cytoplasm, nucleus or p-bodies of yeast pka subunits under different growth conditions
url	http://hdl.handle.net/20.500.12110/paper_01719335_v89_n4_p339_Tudisca
work_keys_str_mv	AT tudiscav differentiallocalizationtocytoplasmnucleusorpbodiesofyeastpkasubunitsunderdifferentgrowthconditions AT recouvreuxv differentiallocalizationtocytoplasmnucleusorpbodiesofyeastpkasubunitsunderdifferentgrowthconditions AT morenos differentiallocalizationtocytoplasmnucleusorpbodiesofyeastpkasubunitsunderdifferentgrowthconditions AT boymarcottee differentiallocalizationtocytoplasmnucleusorpbodiesofyeastpkasubunitsunderdifferentgrowthconditions AT jacquetm differentiallocalizationtocytoplasmnucleusorpbodiesofyeastpkasubunitsunderdifferentgrowthconditions AT portelap differentiallocalizationtocytoplasmnucleusorpbodiesofyeastpkasubunitsunderdifferentgrowthconditions
_version_	1807322522294681600

Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions

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