Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)

Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contr...

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Autores principales: López Gialdi, A.I., Moschen, S., Villán, C.S., López Fernández, M.P., Maldonado, S., Paniego, N., Heinz, R.A., Fernandez, P.
Formato: JOUR
Materias:
Flow cytometry
Helianthus annuus L.
Leaf senescence
Physiological analysis
QPCR
TUNEL
genetics
genotype
growth, development and aging
plant leaf
sunflower
Genotype
Helianthus
Plant Leaves
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p40_LopezGialdi
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01689452_v250_n_p40_LopezGialdi
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spelling todo:paper_01689452_v250_n_p40_LopezGialdi2023-10-03T15:06:52Z Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) López Gialdi, A.I. Moschen, S. Villán, C.S. López Fernández, M.P. Maldonado, S. Paniego, N. Heinz, R.A. Fernandez, P. Flow cytometry Helianthus annuus L. Leaf senescence Physiological analysis QPCR TUNEL genetics genotype growth, development and aging plant leaf sunflower Genotype Helianthus Plant Leaves Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables. © 2016 Elsevier Ireland Ltd. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p40_LopezGialdi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Flow cytometry
Helianthus annuus L.
Leaf senescence
Physiological analysis
QPCR
TUNEL
genetics
genotype
growth, development and aging
plant leaf
sunflower
Genotype
Helianthus
Plant Leaves
spellingShingle Flow cytometry
Helianthus annuus L.
Leaf senescence
Physiological analysis
QPCR
TUNEL
genetics
genotype
growth, development and aging
plant leaf
sunflower
Genotype
Helianthus
Plant Leaves
López Gialdi, A.I.
Moschen, S.
Villán, C.S.
López Fernández, M.P.
Maldonado, S.
Paniego, N.
Heinz, R.A.
Fernandez, P.
Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
topic_facet Flow cytometry
Helianthus annuus L.
Leaf senescence
Physiological analysis
QPCR
TUNEL
genetics
genotype
growth, development and aging
plant leaf
sunflower
Genotype
Helianthus
Plant Leaves
description Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables. © 2016 Elsevier Ireland Ltd.
format JOUR
author López Gialdi, A.I.
Moschen, S.
Villán, C.S.
López Fernández, M.P.
Maldonado, S.
Paniego, N.
Heinz, R.A.
Fernandez, P.
author_facet López Gialdi, A.I.
Moschen, S.
Villán, C.S.
López Fernández, M.P.
Maldonado, S.
Paniego, N.
Heinz, R.A.
Fernandez, P.
author_sort López Gialdi, A.I.
title Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_short Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_full Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_fullStr Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_full_unstemmed Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_sort identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (helianthus annuus l.)
url http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p40_LopezGialdi
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