Involvement of cytoskeleton in Junín virus entry

The early events in Junín virus (JUNV) infection are not thoroughly understood. We have previously shown that JUNV enter cells by clathrin-mediated endocytosis. In this report we examine the role of microfilaments and microtubules during early virus infection. Inhibitory effects of drugs affecting m...

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Autores principales:	Martinez, M.G., Cordo, S.M., Candurra, N.A.
Formato:	JOUR
Materias:	Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Depsipeptides Humans Thiazolidines Vero Cells Virus Internalization Arenavirus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01681702_v138_n1-2_p17_Martinez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01681702_v138_n1-2_p17_Martinez
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spelling	todo:paper_01681702_v138_n1-2_p17_Martinez2023-10-03T15:06:10Z Involvement of cytoskeleton in Junín virus entry Martinez, M.G. Cordo, S.M. Candurra, N.A. Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Cytoskeleton Depsipeptides Humans Junin virus Thiazolidines Vero Cells Virus Internalization Arenavirus Junin virus The early events in Junín virus (JUNV) infection are not thoroughly understood. We have previously shown that JUNV enter cells by clathrin-mediated endocytosis. In this report we examine the role of microfilaments and microtubules during early virus infection. Inhibitory effects of drugs affecting main cytoskeletal components on JUNV entry into Vero cells were analyzed. Drugs that disrupted microfilaments or stabilized microtubules inhibited early steps of virus entry. In contrast, drugs that stabilized microfilaments or depolymerized microtubules were not able to block virus entry very efficiently. Furthermore, real time PCR was performed to detect viral entry and we found more than 10-fold less RNA when microfilaments were depolymerized while a 100-fold diminution was seen when microtubules were stabilized. Taken together our results demonstrate that JUNV relies on an intact actin network during early infection in Vero cells while a dynamic microtubule network is also needed. This represents an important contribution to the characterization of arenavirus multiplication cycle. © 2008 Elsevier B.V. All rights reserved. Fil:Martinez, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cordo, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v138_n1-2_p17_Martinez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Cytoskeleton Depsipeptides Humans Junin virus Thiazolidines Vero Cells Virus Internalization Arenavirus Junin virus
spellingShingle	Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Cytoskeleton Depsipeptides Humans Junin virus Thiazolidines Vero Cells Virus Internalization Arenavirus Junin virus Martinez, M.G. Cordo, S.M. Candurra, N.A. Involvement of cytoskeleton in Junín virus entry
topic_facet	Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Cytoskeleton Depsipeptides Humans Junin virus Thiazolidines Vero Cells Virus Internalization Arenavirus Junin virus
description	The early events in Junín virus (JUNV) infection are not thoroughly understood. We have previously shown that JUNV enter cells by clathrin-mediated endocytosis. In this report we examine the role of microfilaments and microtubules during early virus infection. Inhibitory effects of drugs affecting main cytoskeletal components on JUNV entry into Vero cells were analyzed. Drugs that disrupted microfilaments or stabilized microtubules inhibited early steps of virus entry. In contrast, drugs that stabilized microfilaments or depolymerized microtubules were not able to block virus entry very efficiently. Furthermore, real time PCR was performed to detect viral entry and we found more than 10-fold less RNA when microfilaments were depolymerized while a 100-fold diminution was seen when microtubules were stabilized. Taken together our results demonstrate that JUNV relies on an intact actin network during early infection in Vero cells while a dynamic microtubule network is also needed. This represents an important contribution to the characterization of arenavirus multiplication cycle. © 2008 Elsevier B.V. All rights reserved.
format	JOUR
author	Martinez, M.G. Cordo, S.M. Candurra, N.A.
author_facet	Martinez, M.G. Cordo, S.M. Candurra, N.A.
author_sort	Martinez, M.G.
title	Involvement of cytoskeleton in Junín virus entry
title_short	Involvement of cytoskeleton in Junín virus entry
title_full	Involvement of cytoskeleton in Junín virus entry
title_fullStr	Involvement of cytoskeleton in Junín virus entry
title_full_unstemmed	Involvement of cytoskeleton in Junín virus entry
title_sort	involvement of cytoskeleton in junín virus entry
url	http://hdl.handle.net/20.500.12110/paper_01681702_v138_n1-2_p17_Martinez
work_keys_str_mv	AT martinezmg involvementofcytoskeletoninjuninvirusentry AT cordosm involvementofcytoskeletoninjuninvirusentry AT candurrana involvementofcytoskeletoninjuninvirusentry
_version_	1807317977866960896

Involvement of cytoskeleton in Junín virus entry

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