Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro
In the present paper the in vitro antiviral activity of dehydroepiandrosterone (DHEA), epiandrosterone (EA) and 16 synthetic derivatives against Junin virus (JUNV) replication in Vero cells was studied. DHEA and EA caused a selective inhibition of the replication of JUNV and other members of the Are...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01681702_v135_n2_p203_Acosta |
| Aporte de: |
| id |
todo:paper_01681702_v135_n2_p203_Acosta |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01681702_v135_n2_p203_Acosta2023-10-03T15:06:09Z Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro Acosta, E.G. Bruttomesso, A.C. Bisceglia, J.A. Wachsman, M.B. Galagovsky, L.R. Castilla, V. Antiviral activity Arenavirus Dehydroepiandrosterone DHEA Junin virus Steroid 17 oxo 5alpha androstan 3beta yl acetate 17 oxo 5alpha androstan 3beta,16beta yl diacetate 17 oxo 5alpha androstan 5 en 3beta,16alpha yl diacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta,16alpha yl diacetate 17 oxoandrost 5 en 3beta,16beta yl diacetate 17,17 ethylendioxy 3beta androstanol 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxy 3beta,6alpha androstanediol 17,17 ethylendioxyandrost 15 en 3beta ol 17,17 ethylendioxyandrost 5 en 3beta ol 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxyandrost 15 en 17 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one epiandrosterone glycoprotein g1 prasterone ribavirin virus protein animal cell antiviral activity Arenavirus article controlled study cytotoxicity drug inhibition in vitro study Junin virus nonhuman Pichinde arenavirus priority journal protein synthesis Vero cell virus particle virus replication virus transcription Androsterone Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Junin virus Structure-Activity Relationship Vero Cells Viral Proteins Virus Replication Arenaviridae Arenavirus Junin virus Pichinde virus Tacaribe virus In the present paper the in vitro antiviral activity of dehydroepiandrosterone (DHEA), epiandrosterone (EA) and 16 synthetic derivatives against Junin virus (JUNV) replication in Vero cells was studied. DHEA and EA caused a selective inhibition of the replication of JUNV and other members of the Arenaviridae family such as Pichinde virus and Tacaribe virus. The compounds were not virucidal to cell-free JUNV. The impairment of viral replication was not due to an inhibitory effect of the steroids on virus adsorption or internalization. An inhibitory effect of the compounds on JUNV protein synthesis and both intracellular and extracellular virus production was demonstrated. A partial inhibitory action on cell surface expression of JUNV glycoprotein G1 was also detected on DHEA- and EA-treated cultures. Like DHEA and EA, three compounds obtained from EA by chemical synthesis showed selectivity indexes higher than ribavirin, the only antiviral compound that has shown partial efficacy against arenavirus infections. © 2008 Elsevier B.V. All rights reserved. Fil:Acosta, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bruttomesso, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v135_n2_p203_Acosta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral activity Arenavirus Dehydroepiandrosterone DHEA Junin virus Steroid 17 oxo 5alpha androstan 3beta yl acetate 17 oxo 5alpha androstan 3beta,16beta yl diacetate 17 oxo 5alpha androstan 5 en 3beta,16alpha yl diacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta,16alpha yl diacetate 17 oxoandrost 5 en 3beta,16beta yl diacetate 17,17 ethylendioxy 3beta androstanol 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxy 3beta,6alpha androstanediol 17,17 ethylendioxyandrost 15 en 3beta ol 17,17 ethylendioxyandrost 5 en 3beta ol 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxyandrost 15 en 17 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one epiandrosterone glycoprotein g1 prasterone ribavirin virus protein animal cell antiviral activity Arenavirus article controlled study cytotoxicity drug inhibition in vitro study Junin virus nonhuman Pichinde arenavirus priority journal protein synthesis Vero cell virus particle virus replication virus transcription Androsterone Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Junin virus Structure-Activity Relationship Vero Cells Viral Proteins Virus Replication Arenaviridae Arenavirus Junin virus Pichinde virus Tacaribe virus |
| spellingShingle |
Antiviral activity Arenavirus Dehydroepiandrosterone DHEA Junin virus Steroid 17 oxo 5alpha androstan 3beta yl acetate 17 oxo 5alpha androstan 3beta,16beta yl diacetate 17 oxo 5alpha androstan 5 en 3beta,16alpha yl diacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta,16alpha yl diacetate 17 oxoandrost 5 en 3beta,16beta yl diacetate 17,17 ethylendioxy 3beta androstanol 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxy 3beta,6alpha androstanediol 17,17 ethylendioxyandrost 15 en 3beta ol 17,17 ethylendioxyandrost 5 en 3beta ol 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxyandrost 15 en 17 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one epiandrosterone glycoprotein g1 prasterone ribavirin virus protein animal cell antiviral activity Arenavirus article controlled study cytotoxicity drug inhibition in vitro study Junin virus nonhuman Pichinde arenavirus priority journal protein synthesis Vero cell virus particle virus replication virus transcription Androsterone Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Junin virus Structure-Activity Relationship Vero Cells Viral Proteins Virus Replication Arenaviridae Arenavirus Junin virus Pichinde virus Tacaribe virus Acosta, E.G. Bruttomesso, A.C. Bisceglia, J.A. Wachsman, M.B. Galagovsky, L.R. Castilla, V. Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro |
| topic_facet |
Antiviral activity Arenavirus Dehydroepiandrosterone DHEA Junin virus Steroid 17 oxo 5alpha androstan 3beta yl acetate 17 oxo 5alpha androstan 3beta,16beta yl diacetate 17 oxo 5alpha androstan 5 en 3beta,16alpha yl diacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta,16alpha yl diacetate 17 oxoandrost 5 en 3beta,16beta yl diacetate 17,17 ethylendioxy 3beta androstanol 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxy 3beta,6alpha androstanediol 17,17 ethylendioxyandrost 15 en 3beta ol 17,17 ethylendioxyandrost 5 en 3beta ol 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxyandrost 15 en 17 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one epiandrosterone glycoprotein g1 prasterone ribavirin virus protein animal cell antiviral activity Arenavirus article controlled study cytotoxicity drug inhibition in vitro study Junin virus nonhuman Pichinde arenavirus priority journal protein synthesis Vero cell virus particle virus replication virus transcription Androsterone Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Junin virus Structure-Activity Relationship Vero Cells Viral Proteins Virus Replication Arenaviridae Arenavirus Junin virus Pichinde virus Tacaribe virus |
| description |
In the present paper the in vitro antiviral activity of dehydroepiandrosterone (DHEA), epiandrosterone (EA) and 16 synthetic derivatives against Junin virus (JUNV) replication in Vero cells was studied. DHEA and EA caused a selective inhibition of the replication of JUNV and other members of the Arenaviridae family such as Pichinde virus and Tacaribe virus. The compounds were not virucidal to cell-free JUNV. The impairment of viral replication was not due to an inhibitory effect of the steroids on virus adsorption or internalization. An inhibitory effect of the compounds on JUNV protein synthesis and both intracellular and extracellular virus production was demonstrated. A partial inhibitory action on cell surface expression of JUNV glycoprotein G1 was also detected on DHEA- and EA-treated cultures. Like DHEA and EA, three compounds obtained from EA by chemical synthesis showed selectivity indexes higher than ribavirin, the only antiviral compound that has shown partial efficacy against arenavirus infections. © 2008 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Acosta, E.G. Bruttomesso, A.C. Bisceglia, J.A. Wachsman, M.B. Galagovsky, L.R. Castilla, V. |
| author_facet |
Acosta, E.G. Bruttomesso, A.C. Bisceglia, J.A. Wachsman, M.B. Galagovsky, L.R. Castilla, V. |
| author_sort |
Acosta, E.G. |
| title |
Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro |
| title_short |
Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro |
| title_full |
Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro |
| title_fullStr |
Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro |
| title_full_unstemmed |
Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro |
| title_sort |
dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit junin virus replication in vitro |
| url |
http://hdl.handle.net/20.500.12110/paper_01681702_v135_n2_p203_Acosta |
| work_keys_str_mv |
AT acostaeg dehydroepiandrosteroneepiandrosteroneandsyntheticderivativesinhibitjuninvirusreplicationinvitro AT bruttomessoac dehydroepiandrosteroneepiandrosteroneandsyntheticderivativesinhibitjuninvirusreplicationinvitro AT biscegliaja dehydroepiandrosteroneepiandrosteroneandsyntheticderivativesinhibitjuninvirusreplicationinvitro AT wachsmanmb dehydroepiandrosteroneepiandrosteroneandsyntheticderivativesinhibitjuninvirusreplicationinvitro AT galagovskylr dehydroepiandrosteroneepiandrosteroneandsyntheticderivativesinhibitjuninvirusreplicationinvitro AT castillav dehydroepiandrosteroneepiandrosteroneandsyntheticderivativesinhibitjuninvirusreplicationinvitro |
| _version_ |
1782030470637158400 |