Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk
Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a gliotoxinogenic species into section Fumigati commonly found in contaminated animal environments. In dairy herds, exposed areas of lactating cows, as mammalian glandule, can be easily contaminated by them. Thi...
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todo:paper_01681605_v275_n_p1_Pena2023-10-03T15:05:56Z Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk Pena, G.A. Alonso, V. Manini, M.V. Pellegrino, M. Cavaglieri, L.R. A. fumigatus sensu stricto Genetic variability estimation Molecular typing Raw cow milk gliotoxin Argentina Article Aspergillus fumigatus cluster analysis fungal strain fungus isolation genetic distance genetic variability milk molecular typing nonhuman parsimony analysis random amplified polymorphic DNA raw food Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a gliotoxinogenic species into section Fumigati commonly found in contaminated animal environments. In dairy herds, exposed areas of lactating cows, as mammalian glandule, can be easily contaminated by them. This study was aimed to identify A. fumigatus sensu lato strains (identified based on morphology) isolated from raw cow milk at species level, by morphological and molecular techniques, and to estimate their genetic variability. Forty-five A. fumigatus strains showed similar RAPD profiles (generated with PELF and URP1F primers) to each other and to A. fumigatus sensu stricto reference strains; also, they were almost identical to clinical human and feed-borne A. fumigatus strains included in the assay, since their similarity coefficient ranged from 0.7 to 1.00. Therefore, all strains were characterized as belonging to A. fumigatus sensu stricto species. This result was supported by sequencing the benA gene of selected strains and by maximum parsimony analysis. In addition, RAPD fingerprinting demonstrated intra-specific genetic variability into the A. fumigatus sensu stricto cluster. The results found in this study strengthen the fact that A. fumigatus sensu stricto is the predominant species in the Aspergillus section Fumigati found in animal environments such as dairy herd environments, while other species such as A. novofumigatus, A. fumigatiaffinis, A. udagawae and A. lentulus may be rarely isolated. Since no differences between animal and human strains were observed they can become pathogenic also for farm handlers’. Moreover, the presence of A. fumigatus sensu stricto in raw cow milk is probably a very important risk factor since milk and its by-products are generally indented for human consumption, then gliotoxin could be transferred to them. © 2018 JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681605_v275_n_p1_Pena |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
A. fumigatus sensu stricto Genetic variability estimation Molecular typing Raw cow milk gliotoxin Argentina Article Aspergillus fumigatus cluster analysis fungal strain fungus isolation genetic distance genetic variability milk molecular typing nonhuman parsimony analysis random amplified polymorphic DNA raw food |
| spellingShingle |
A. fumigatus sensu stricto Genetic variability estimation Molecular typing Raw cow milk gliotoxin Argentina Article Aspergillus fumigatus cluster analysis fungal strain fungus isolation genetic distance genetic variability milk molecular typing nonhuman parsimony analysis random amplified polymorphic DNA raw food Pena, G.A. Alonso, V. Manini, M.V. Pellegrino, M. Cavaglieri, L.R. Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk |
| topic_facet |
A. fumigatus sensu stricto Genetic variability estimation Molecular typing Raw cow milk gliotoxin Argentina Article Aspergillus fumigatus cluster analysis fungal strain fungus isolation genetic distance genetic variability milk molecular typing nonhuman parsimony analysis random amplified polymorphic DNA raw food |
| description |
Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a gliotoxinogenic species into section Fumigati commonly found in contaminated animal environments. In dairy herds, exposed areas of lactating cows, as mammalian glandule, can be easily contaminated by them. This study was aimed to identify A. fumigatus sensu lato strains (identified based on morphology) isolated from raw cow milk at species level, by morphological and molecular techniques, and to estimate their genetic variability. Forty-five A. fumigatus strains showed similar RAPD profiles (generated with PELF and URP1F primers) to each other and to A. fumigatus sensu stricto reference strains; also, they were almost identical to clinical human and feed-borne A. fumigatus strains included in the assay, since their similarity coefficient ranged from 0.7 to 1.00. Therefore, all strains were characterized as belonging to A. fumigatus sensu stricto species. This result was supported by sequencing the benA gene of selected strains and by maximum parsimony analysis. In addition, RAPD fingerprinting demonstrated intra-specific genetic variability into the A. fumigatus sensu stricto cluster. The results found in this study strengthen the fact that A. fumigatus sensu stricto is the predominant species in the Aspergillus section Fumigati found in animal environments such as dairy herd environments, while other species such as A. novofumigatus, A. fumigatiaffinis, A. udagawae and A. lentulus may be rarely isolated. Since no differences between animal and human strains were observed they can become pathogenic also for farm handlers’. Moreover, the presence of A. fumigatus sensu stricto in raw cow milk is probably a very important risk factor since milk and its by-products are generally indented for human consumption, then gliotoxin could be transferred to them. © 2018 |
| format |
JOUR |
| author |
Pena, G.A. Alonso, V. Manini, M.V. Pellegrino, M. Cavaglieri, L.R. |
| author_facet |
Pena, G.A. Alonso, V. Manini, M.V. Pellegrino, M. Cavaglieri, L.R. |
| author_sort |
Pena, G.A. |
| title |
Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk |
| title_short |
Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk |
| title_full |
Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk |
| title_fullStr |
Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk |
| title_full_unstemmed |
Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk |
| title_sort |
molecular characterization of aspergillus fumigatus isolated from raw cow milk in argentina: molecular typing of a. fumigatus from raw cow milk |
| url |
http://hdl.handle.net/20.500.12110/paper_01681605_v275_n_p1_Pena |
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