Comparison of lactococcal bacteriophage isolated in the United States and Argentina

Bacteriophage of Lactococcus lactis ssp. lactis and ssp. cremoris, isolated in the United States and Argentina, were compared with respect to host range, adsorption, latent period, burst size and immunological cross-reactivity. Only 1 out of 13 U.S. culture isolates was sensitive to Argentinian phag...

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Autores principales: de Fabrizio, S.V., Ledford, R.A., Shieh, Y.S.C., Brown, J., Parada, J.L.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01681605_v13_n4_p285_deFabrizio
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spelling todo:paper_01681605_v13_n4_p285_deFabrizio2023-10-03T15:05:48Z Comparison of lactococcal bacteriophage isolated in the United States and Argentina de Fabrizio, S.V. Ledford, R.A. Shieh, Y.S.C. Brown, J. Parada, J.L. Adsorption Bacteriophage Burst size Host range Immunological cross-reactivity Lactococcus lactis antibody article bacteriophage cross reaction Lactococcus lactis mutant nonhuman priority journal Absorption Argentina Bacteriophages Comparative Study Cross Reactions Culture Media Lactococcus lactis Mutation Plaque Assay Support, Non-U.S. Gov't United States Bacteriophage of Lactococcus lactis ssp. lactis and ssp. cremoris, isolated in the United States and Argentina, were compared with respect to host range, adsorption, latent period, burst size and immunological cross-reactivity. Only 1 out of 13 U.S. culture isolates was sensitive to Argentinian phage. Argentinian L. lactis ssp. lactis C2 mutants were resistant to 13 U.S. phage isolates (4 prolate and 9 isometric). While Argentinian phage Stl-3 multiplied on U.S. culture isolate 59-1, low adsorption (38%) and insignificant burst size and latent period data were evident. Antisera prepared against U.S. phage D59-1 (prolate) and F4-1 (isometric) neutralized the lytic activities of all Argentinian prolate phage although the F4-1 antiserum was less effective. The data suggest homology especially between U.S. phage D59-1 and the Argentinian phage. © 1991. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681605_v13_n4_p285_deFabrizio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Adsorption
Bacteriophage
Burst size
Host range
Immunological cross-reactivity
Lactococcus lactis
antibody
article
bacteriophage
cross reaction
Lactococcus lactis
mutant
nonhuman
priority journal
Absorption
Argentina
Bacteriophages
Comparative Study
Cross Reactions
Culture Media
Lactococcus lactis
Mutation
Plaque Assay
Support, Non-U.S. Gov't
United States
spellingShingle Adsorption
Bacteriophage
Burst size
Host range
Immunological cross-reactivity
Lactococcus lactis
antibody
article
bacteriophage
cross reaction
Lactococcus lactis
mutant
nonhuman
priority journal
Absorption
Argentina
Bacteriophages
Comparative Study
Cross Reactions
Culture Media
Lactococcus lactis
Mutation
Plaque Assay
Support, Non-U.S. Gov't
United States
de Fabrizio, S.V.
Ledford, R.A.
Shieh, Y.S.C.
Brown, J.
Parada, J.L.
Comparison of lactococcal bacteriophage isolated in the United States and Argentina
topic_facet Adsorption
Bacteriophage
Burst size
Host range
Immunological cross-reactivity
Lactococcus lactis
antibody
article
bacteriophage
cross reaction
Lactococcus lactis
mutant
nonhuman
priority journal
Absorption
Argentina
Bacteriophages
Comparative Study
Cross Reactions
Culture Media
Lactococcus lactis
Mutation
Plaque Assay
Support, Non-U.S. Gov't
United States
description Bacteriophage of Lactococcus lactis ssp. lactis and ssp. cremoris, isolated in the United States and Argentina, were compared with respect to host range, adsorption, latent period, burst size and immunological cross-reactivity. Only 1 out of 13 U.S. culture isolates was sensitive to Argentinian phage. Argentinian L. lactis ssp. lactis C2 mutants were resistant to 13 U.S. phage isolates (4 prolate and 9 isometric). While Argentinian phage Stl-3 multiplied on U.S. culture isolate 59-1, low adsorption (38%) and insignificant burst size and latent period data were evident. Antisera prepared against U.S. phage D59-1 (prolate) and F4-1 (isometric) neutralized the lytic activities of all Argentinian prolate phage although the F4-1 antiserum was less effective. The data suggest homology especially between U.S. phage D59-1 and the Argentinian phage. © 1991.
format JOUR
author de Fabrizio, S.V.
Ledford, R.A.
Shieh, Y.S.C.
Brown, J.
Parada, J.L.
author_facet de Fabrizio, S.V.
Ledford, R.A.
Shieh, Y.S.C.
Brown, J.
Parada, J.L.
author_sort de Fabrizio, S.V.
title Comparison of lactococcal bacteriophage isolated in the United States and Argentina
title_short Comparison of lactococcal bacteriophage isolated in the United States and Argentina
title_full Comparison of lactococcal bacteriophage isolated in the United States and Argentina
title_fullStr Comparison of lactococcal bacteriophage isolated in the United States and Argentina
title_full_unstemmed Comparison of lactococcal bacteriophage isolated in the United States and Argentina
title_sort comparison of lactococcal bacteriophage isolated in the united states and argentina
url http://hdl.handle.net/20.500.12110/paper_01681605_v13_n4_p285_deFabrizio
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AT ledfordra comparisonoflactococcalbacteriophageisolatedintheunitedstatesandargentina
AT shiehysc comparisonoflactococcalbacteriophageisolatedintheunitedstatesandargentina
AT brownj comparisonoflactococcalbacteriophageisolatedintheunitedstatesandargentina
AT paradajl comparisonoflactococcalbacteriophageisolatedintheunitedstatesandargentina
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