New method for electroporation of Lactobacillus species grown in high salt

We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per micr...

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Autores principales: Palomino, M.M., Allievi, M.C., Prado-Acosta, M., Sanchez-Rivas, C., Ruzal, S.M.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01677012_v83_n2_p164_Palomino
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