New method for electroporation of Lactobacillus species grown in high salt

We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per micr...

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Autores principales: Palomino, M.M., Allievi, M.C., Prado-Acosta, M., Sanchez-Rivas, C., Ruzal, S.M.
Formato: JOUR
Materias:
Electroporation
High salt growth
Lactobacillus
plasmid DNA
sodium chloride
article
bacterial cell wall
bacterial growth
bacterial strain
bacterium transformation
controlled study
cryopreservation
electroporation
fermentation
Lactobacillus acidophilus
Lactobacillus delbrueckii
Lactobacillus paracasei
Lactobacillus plantarum
nonhuman
priority journal
salt tolerance
Lactobacillus casei
Lactobacillus delbrueckii subsp. lactis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01677012_v83_n2_p164_Palomino
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01677012_v83_n2_p164_Palomino
record_format dspace
spelling todo:paper_01677012_v83_n2_p164_Palomino2023-10-03T15:05:11Z New method for electroporation of Lactobacillus species grown in high salt Palomino, M.M. Allievi, M.C. Prado-Acosta, M. Sanchez-Rivas, C. Ruzal, S.M. Electroporation High salt growth Lactobacillus plasmid DNA sodium chloride article bacterial cell wall bacterial growth bacterial strain bacterium transformation controlled study cryopreservation electroporation fermentation Lactobacillus Lactobacillus acidophilus Lactobacillus delbrueckii Lactobacillus paracasei Lactobacillus plantarum nonhuman priority journal salt tolerance Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lactobacillus delbrueckii subsp. lactis Lactobacillus paracasei Lactobacillus plantarum We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per microgram of plasmid DNA was achieved following growth in the presence of 0.9M NaCl. Plasmids of different sizes and replication origins were also similarly transformed. These competent cells could be used either directly or stored frozen, up to 1month, for future use, with similar efficiency. This protocol was assayed with different Lactobacillus species: L. delbrueckii subsp. lactis, L. paracasei, L. plantarum and L. acidophilus, and it was found that they were transformed with similar efficiency. © 2010 Elsevier B.V. Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Prado-Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01677012_v83_n2_p164_Palomino
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Electroporation
High salt growth
Lactobacillus
plasmid DNA
sodium chloride
article
bacterial cell wall
bacterial growth
bacterial strain
bacterium transformation
controlled study
cryopreservation
electroporation
fermentation
Lactobacillus
Lactobacillus acidophilus
Lactobacillus delbrueckii
Lactobacillus paracasei
Lactobacillus plantarum
nonhuman
priority journal
salt tolerance
Lactobacillus
Lactobacillus acidophilus
Lactobacillus casei
Lactobacillus delbrueckii subsp. lactis
Lactobacillus paracasei
Lactobacillus plantarum
spellingShingle Electroporation
High salt growth
Lactobacillus
plasmid DNA
sodium chloride
article
bacterial cell wall
bacterial growth
bacterial strain
bacterium transformation
controlled study
cryopreservation
electroporation
fermentation
Lactobacillus
Lactobacillus acidophilus
Lactobacillus delbrueckii
Lactobacillus paracasei
Lactobacillus plantarum
nonhuman
priority journal
salt tolerance
Lactobacillus
Lactobacillus acidophilus
Lactobacillus casei
Lactobacillus delbrueckii subsp. lactis
Lactobacillus paracasei
Lactobacillus plantarum
Palomino, M.M.
Allievi, M.C.
Prado-Acosta, M.
Sanchez-Rivas, C.
Ruzal, S.M.
New method for electroporation of Lactobacillus species grown in high salt
topic_facet Electroporation
High salt growth
Lactobacillus
plasmid DNA
sodium chloride
article
bacterial cell wall
bacterial growth
bacterial strain
bacterium transformation
controlled study
cryopreservation
electroporation
fermentation
Lactobacillus
Lactobacillus acidophilus
Lactobacillus delbrueckii
Lactobacillus paracasei
Lactobacillus plantarum
nonhuman
priority journal
salt tolerance
Lactobacillus
Lactobacillus acidophilus
Lactobacillus casei
Lactobacillus delbrueckii subsp. lactis
Lactobacillus paracasei
Lactobacillus plantarum
description We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per microgram of plasmid DNA was achieved following growth in the presence of 0.9M NaCl. Plasmids of different sizes and replication origins were also similarly transformed. These competent cells could be used either directly or stored frozen, up to 1month, for future use, with similar efficiency. This protocol was assayed with different Lactobacillus species: L. delbrueckii subsp. lactis, L. paracasei, L. plantarum and L. acidophilus, and it was found that they were transformed with similar efficiency. © 2010 Elsevier B.V.
format JOUR
author Palomino, M.M.
Allievi, M.C.
Prado-Acosta, M.
Sanchez-Rivas, C.
Ruzal, S.M.
author_facet Palomino, M.M.
Allievi, M.C.
Prado-Acosta, M.
Sanchez-Rivas, C.
Ruzal, S.M.
author_sort Palomino, M.M.
title New method for electroporation of Lactobacillus species grown in high salt
title_short New method for electroporation of Lactobacillus species grown in high salt
title_full New method for electroporation of Lactobacillus species grown in high salt
title_fullStr New method for electroporation of Lactobacillus species grown in high salt
title_full_unstemmed New method for electroporation of Lactobacillus species grown in high salt
title_sort new method for electroporation of lactobacillus species grown in high salt
url http://hdl.handle.net/20.500.12110/paper_01677012_v83_n2_p164_Palomino
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AT allievimc newmethodforelectroporationoflactobacillusspeciesgrowninhighsalt
AT pradoacostam newmethodforelectroporationoflactobacillusspeciesgrowninhighsalt
AT sanchezrivasc newmethodforelectroporationoflactobacillusspeciesgrowninhighsalt
AT ruzalsm newmethodforelectroporationoflactobacillusspeciesgrowninhighsalt
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