Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells
Human breast tumor cells MCF-7 were grown during 5 days in the presence of Adriamycin and the IC50 was 50 nM with the highest sublethal concentration 0.1 μM. At this latter concentration Adriamycin produced a complete inhibition of cell division and a partial reversion to a normal breast epithelial...
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todo:paper_01676806_v17_n2_p145_Bustamante2023-10-03T15:05:04Z Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells Bustamante, J. Galleano, M. Medrano, E.E. Boveris, A. Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured Human breast tumor cells MCF-7 were grown during 5 days in the presence of Adriamycin and the IC50 was 50 nM with the highest sublethal concentration 0.1 μM. At this latter concentration Adriamycin produced a complete inhibition of cell division and a partial reversion to a normal breast epithelial appearance. Similar effects of Adriamycin were observed in cells cultured in the presence of 10% FBS and in a chemically defined medium, with Se-glutathione peroxidase activities of 3.8 and 1.3 U/mg of protein, respectively. Cell size and cell oxygen uptake were increased by 41% and by 50%, respectively, in Adriamycin-treated cells. The spontaneous chemiluminescence of monolayers of intact MCF-7 cells (81 ± 9 cps/mg protein) was increased by 48% in the Adriamycin-treated cultures (120 ± 11 cps/mg of protein) in agreement with a 91% higher concentration of malondialdehyde in the same cultures. Adriamycin treatment produced a 71% increase in the steady state concentration of H202, which was estimated assuming diffusion equilibrium with the external medium, from 1.38 μM in the control cells to 2.38 μM in the treated cells. Cyanide-insensitive respiration was also higher in the cells exposed to the drug than in the control cells. Adriamycin did not affect the activity of the antioxidant enzymes, Cu-Zn and Mn-superoxide dismutase, Se and non-Se-glutathione peroxidase, and catalase. These results contribute to the current hypothesis that oxygen free radicals produced by Adriamycin redox cycling are responsible for at least part of the cytotoxic effects due to this drug. © 1990 Kluwer Academic Publishers. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01676806_v17_n2_p145_Bustamante |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured |
spellingShingle |
Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured Bustamante, J. Galleano, M. Medrano, E.E. Boveris, A. Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells |
topic_facet |
Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured |
description |
Human breast tumor cells MCF-7 were grown during 5 days in the presence of Adriamycin and the IC50 was 50 nM with the highest sublethal concentration 0.1 μM. At this latter concentration Adriamycin produced a complete inhibition of cell division and a partial reversion to a normal breast epithelial appearance. Similar effects of Adriamycin were observed in cells cultured in the presence of 10% FBS and in a chemically defined medium, with Se-glutathione peroxidase activities of 3.8 and 1.3 U/mg of protein, respectively. Cell size and cell oxygen uptake were increased by 41% and by 50%, respectively, in Adriamycin-treated cells. The spontaneous chemiluminescence of monolayers of intact MCF-7 cells (81 ± 9 cps/mg protein) was increased by 48% in the Adriamycin-treated cultures (120 ± 11 cps/mg of protein) in agreement with a 91% higher concentration of malondialdehyde in the same cultures. Adriamycin treatment produced a 71% increase in the steady state concentration of H202, which was estimated assuming diffusion equilibrium with the external medium, from 1.38 μM in the control cells to 2.38 μM in the treated cells. Cyanide-insensitive respiration was also higher in the cells exposed to the drug than in the control cells. Adriamycin did not affect the activity of the antioxidant enzymes, Cu-Zn and Mn-superoxide dismutase, Se and non-Se-glutathione peroxidase, and catalase. These results contribute to the current hypothesis that oxygen free radicals produced by Adriamycin redox cycling are responsible for at least part of the cytotoxic effects due to this drug. © 1990 Kluwer Academic Publishers. |
format |
JOUR |
author |
Bustamante, J. Galleano, M. Medrano, E.E. Boveris, A. |
author_facet |
Bustamante, J. Galleano, M. Medrano, E.E. Boveris, A. |
author_sort |
Bustamante, J. |
title |
Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells |
title_short |
Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells |
title_full |
Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells |
title_fullStr |
Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells |
title_full_unstemmed |
Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells |
title_sort |
adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells |
url |
http://hdl.handle.net/20.500.12110/paper_01676806_v17_n2_p145_Bustamante |
work_keys_str_mv |
AT bustamantej adriamycineffectsonhydroperoxidemetabolismandgrowthofhumanbreasttumorcells AT galleanom adriamycineffectsonhydroperoxidemetabolismandgrowthofhumanbreasttumorcells AT medranoee adriamycineffectsonhydroperoxidemetabolismandgrowthofhumanbreasttumorcells AT boverisa adriamycineffectsonhydroperoxidemetabolismandgrowthofhumanbreasttumorcells |
_version_ |
1782024210836619264 |