Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice
We have determined the presence of transforming growth factor-β (TGF-β)-like polypeptides in mammary adenocarcinomas induced by medroxyprogesterone acetate (MPA) in BALB/c mice. In hormone-dependent tumors (HD) from nontreated and MPA-treated mice a high molecular weight (43 kDa) transforming activi...
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todo:paper_01676806_v16_n1_p29_Elizalde2023-10-03T15:05:04Z Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice Elizalde, P.V. Lanari, C. Kordon, E. Tezón, J. Charreau, E.H. inhibitory growth control mammary adenocarcinomas medroxyprogesterone acetate transforming growth factor-β medroxyprogesterone acetate transforming growth factor beta animal experiment article breast adenocarcinoma female mouse nonhuman subcutaneous drug administration Adenocarcinoma Animal Cell Division Cell Transformation, Neoplastic Chromatography, High Pressure Liquid DNA Replication Drug Interactions Epidermal Growth Factor Fibroblasts Gene Expression Regulation, Neoplastic Insulin Mammary Neoplasms, Experimental Medroxyprogesterone Medroxyprogesterone 17-Acetate Mice Mice, Inbred BALB C Neoplasm Proteins Neoplasms, Hormone-Dependent Rats Support, Non-U.S. Gov't Transforming Growth Factor beta We have determined the presence of transforming growth factor-β (TGF-β)-like polypeptides in mammary adenocarcinomas induced by medroxyprogesterone acetate (MPA) in BALB/c mice. In hormone-dependent tumors (HD) from nontreated and MPA-treated mice a high molecular weight (43 kDa) transforming activity was purified by Bio-Gel P-60 chromatography. This TGF was able to confer the neoplastic phenotype on NRK-49F cells without the addition of epidermal growth factor (EGF), though its activity was potentiated by EGF. It did not compete for binding to the EGF receptor, had no mitogenic activity on monolayer cultures of NRK fibroblasts, and was a potent inhibitor of DNA synthesis induced in these cells by EGF and insulin. In HD and hormone-independent tumors (HI) another TGF with a Mr of 13 kDa was isolated. This transforming activity showed the same biological properties as 43 kDa TGF, with the exception that in the absence of EGF it did not stimulate soft agar growth of NRK-49F cells. The synthesis of both factors in 'in vivo' HD tumors seems to be under MPA control, since it is much lower in HD tumors from MPA-treated mice. Further purification of the 13 and 43 kDa TGFs by hydrophobic interaction HPLC demonstrated that each one eluted in a different position, and that their elution profile differed from the TGF-β from human platelets. The biological activity of the 13 and 43 kDa TGFs was not neutralized by a specific anti-TGF-β antibody. © 1990 Kluwer Academic Publishers. Fil:Elizalde, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanari, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordon, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tezón, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01676806_v16_n1_p29_Elizalde |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
inhibitory growth control mammary adenocarcinomas medroxyprogesterone acetate transforming growth factor-β medroxyprogesterone acetate transforming growth factor beta animal experiment article breast adenocarcinoma female mouse nonhuman subcutaneous drug administration Adenocarcinoma Animal Cell Division Cell Transformation, Neoplastic Chromatography, High Pressure Liquid DNA Replication Drug Interactions Epidermal Growth Factor Fibroblasts Gene Expression Regulation, Neoplastic Insulin Mammary Neoplasms, Experimental Medroxyprogesterone Medroxyprogesterone 17-Acetate Mice Mice, Inbred BALB C Neoplasm Proteins Neoplasms, Hormone-Dependent Rats Support, Non-U.S. Gov't Transforming Growth Factor beta |
| spellingShingle |
inhibitory growth control mammary adenocarcinomas medroxyprogesterone acetate transforming growth factor-β medroxyprogesterone acetate transforming growth factor beta animal experiment article breast adenocarcinoma female mouse nonhuman subcutaneous drug administration Adenocarcinoma Animal Cell Division Cell Transformation, Neoplastic Chromatography, High Pressure Liquid DNA Replication Drug Interactions Epidermal Growth Factor Fibroblasts Gene Expression Regulation, Neoplastic Insulin Mammary Neoplasms, Experimental Medroxyprogesterone Medroxyprogesterone 17-Acetate Mice Mice, Inbred BALB C Neoplasm Proteins Neoplasms, Hormone-Dependent Rats Support, Non-U.S. Gov't Transforming Growth Factor beta Elizalde, P.V. Lanari, C. Kordon, E. Tezón, J. Charreau, E.H. Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice |
| topic_facet |
inhibitory growth control mammary adenocarcinomas medroxyprogesterone acetate transforming growth factor-β medroxyprogesterone acetate transforming growth factor beta animal experiment article breast adenocarcinoma female mouse nonhuman subcutaneous drug administration Adenocarcinoma Animal Cell Division Cell Transformation, Neoplastic Chromatography, High Pressure Liquid DNA Replication Drug Interactions Epidermal Growth Factor Fibroblasts Gene Expression Regulation, Neoplastic Insulin Mammary Neoplasms, Experimental Medroxyprogesterone Medroxyprogesterone 17-Acetate Mice Mice, Inbred BALB C Neoplasm Proteins Neoplasms, Hormone-Dependent Rats Support, Non-U.S. Gov't Transforming Growth Factor beta |
| description |
We have determined the presence of transforming growth factor-β (TGF-β)-like polypeptides in mammary adenocarcinomas induced by medroxyprogesterone acetate (MPA) in BALB/c mice. In hormone-dependent tumors (HD) from nontreated and MPA-treated mice a high molecular weight (43 kDa) transforming activity was purified by Bio-Gel P-60 chromatography. This TGF was able to confer the neoplastic phenotype on NRK-49F cells without the addition of epidermal growth factor (EGF), though its activity was potentiated by EGF. It did not compete for binding to the EGF receptor, had no mitogenic activity on monolayer cultures of NRK fibroblasts, and was a potent inhibitor of DNA synthesis induced in these cells by EGF and insulin. In HD and hormone-independent tumors (HI) another TGF with a Mr of 13 kDa was isolated. This transforming activity showed the same biological properties as 43 kDa TGF, with the exception that in the absence of EGF it did not stimulate soft agar growth of NRK-49F cells. The synthesis of both factors in 'in vivo' HD tumors seems to be under MPA control, since it is much lower in HD tumors from MPA-treated mice. Further purification of the 13 and 43 kDa TGFs by hydrophobic interaction HPLC demonstrated that each one eluted in a different position, and that their elution profile differed from the TGF-β from human platelets. The biological activity of the 13 and 43 kDa TGFs was not neutralized by a specific anti-TGF-β antibody. © 1990 Kluwer Academic Publishers. |
| format |
JOUR |
| author |
Elizalde, P.V. Lanari, C. Kordon, E. Tezón, J. Charreau, E.H. |
| author_facet |
Elizalde, P.V. Lanari, C. Kordon, E. Tezón, J. Charreau, E.H. |
| author_sort |
Elizalde, P.V. |
| title |
Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice |
| title_short |
Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice |
| title_full |
Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice |
| title_fullStr |
Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice |
| title_full_unstemmed |
Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice |
| title_sort |
transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in balb/c mice |
| url |
http://hdl.handle.net/20.500.12110/paper_01676806_v16_n1_p29_Elizalde |
| work_keys_str_mv |
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1807323941938659328 |