Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)

Contamination by 2,4,6-trinitrotoluene (TNT) is a global environmental problem at sites of former explosive production, handling, or storage, and could have deleterious consequences for human and ecological health. We investigated its sublethal effects to Eisenia fetida, using two nonspecific biomar...

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Autores principales:	Fuchs, J., Piola, L., González, E.P., Oneto, M.L., Basack, S., Kesten, E., Casabé, N.
Formato:	JOUR
Materias:	2,4,6-trinitrotoluene (TNT) Biomarkers Coelomocytes Comet assay Eisenia fetida Neutral red retention time Concentration (process) Damage detection Nucleic acids DNA trinitrotoluene biomarker concentration (composition) earthworm sublethal effect analytic method article calculation cell type cell viability celomocyte comet assay contamination controlled study DNA damage DNA repair environmental exposure environmental impact assessment natural red retention time nonhuman Animals Biological Markers Comet Assay Environmental Monitoring Oligochaeta Trinitrotoluene
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01676369_v175_n1-4_p127_Fuchs
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01676369_v175_n1-4_p127_Fuchs2023-10-03T15:04:59Z Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT) Fuchs, J. Piola, L. González, E.P. Oneto, M.L. Basack, S. Kesten, E. Casabé, N. 2,4,6-trinitrotoluene (TNT) Biomarkers Coelomocytes Comet assay Eisenia fetida Neutral red retention time 2,4,6-trinitrotoluene (TNT) Coelomocytes Comet assay Eisenia fetida Neutral red retention time Concentration (process) Damage detection Nucleic acids DNA trinitrotoluene biomarker concentration (composition) DNA earthworm sublethal effect trinitrotoluene analytic method article calculation cell type cell viability celomocyte comet assay contamination controlled study DNA damage DNA repair earthworm environmental exposure environmental impact assessment natural red retention time nonhuman Animals Biological Markers Comet Assay Environmental Monitoring Oligochaeta Trinitrotoluene Eisenia fetida Contamination by 2,4,6-trinitrotoluene (TNT) is a global environmental problem at sites of former explosive production, handling, or storage, and could have deleterious consequences for human and ecological health. We investigated its sublethal effects to Eisenia fetida, using two nonspecific biomarkers. In coelomocytes of earthworms exposed 24, 48, or 72 h, we evaluated DNA damage (comet assay) and neutral red retention time (NRRT), using the filter paper contact test. Both percentage of damage (D%) and calculated damage index showed significant DNA damage at almost all concentrations, at all time points assayed. Along exposure time, two different patterns were observed. At the lower TNT concentrations (0.25-0.5 μg/cm 2 ) an increased DNA migration at 48 h, with a decrease close to initial levels after 72 h exposure, was observed. This decrease could be attributed to activation of the DNA repair system. At higher concentrations (1.0-2.0 μg/cm 2 ), the high DNA damage observed remained constant during the 72 h exposure, suggesting that the rate of DNA repair was not enough to compensate such damage. Analysis of NRRT results showed a significant interaction between time and treatment. After 48 h, a significant decrease was observed at 4.0 μg/cm 2 . After 72 h, NRRT presented a concentration-dependent decrease, significantly different with respect to control at 0.5, 1.0, 2.0, and 4.0 μg/cm 2 . The two assayed methods, performed on the same sample, showed clear responses to sublethal TNT exposure in E. fetida, providing sensitive unspecific biomarkers of cell injury and DNA damage. © 2010 Springer Science+Business Media B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01676369_v175_n1-4_p127_Fuchs
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	2,4,6-trinitrotoluene (TNT) Biomarkers Coelomocytes Comet assay Eisenia fetida Neutral red retention time 2,4,6-trinitrotoluene (TNT) Coelomocytes Comet assay Eisenia fetida Neutral red retention time Concentration (process) Damage detection Nucleic acids DNA trinitrotoluene biomarker concentration (composition) DNA earthworm sublethal effect trinitrotoluene analytic method article calculation cell type cell viability celomocyte comet assay contamination controlled study DNA damage DNA repair earthworm environmental exposure environmental impact assessment natural red retention time nonhuman Animals Biological Markers Comet Assay Environmental Monitoring Oligochaeta Trinitrotoluene Eisenia fetida
spellingShingle	2,4,6-trinitrotoluene (TNT) Biomarkers Coelomocytes Comet assay Eisenia fetida Neutral red retention time 2,4,6-trinitrotoluene (TNT) Coelomocytes Comet assay Eisenia fetida Neutral red retention time Concentration (process) Damage detection Nucleic acids DNA trinitrotoluene biomarker concentration (composition) DNA earthworm sublethal effect trinitrotoluene analytic method article calculation cell type cell viability celomocyte comet assay contamination controlled study DNA damage DNA repair earthworm environmental exposure environmental impact assessment natural red retention time nonhuman Animals Biological Markers Comet Assay Environmental Monitoring Oligochaeta Trinitrotoluene Eisenia fetida Fuchs, J. Piola, L. González, E.P. Oneto, M.L. Basack, S. Kesten, E. Casabé, N. Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)
topic_facet	2,4,6-trinitrotoluene (TNT) Biomarkers Coelomocytes Comet assay Eisenia fetida Neutral red retention time 2,4,6-trinitrotoluene (TNT) Coelomocytes Comet assay Eisenia fetida Neutral red retention time Concentration (process) Damage detection Nucleic acids DNA trinitrotoluene biomarker concentration (composition) DNA earthworm sublethal effect trinitrotoluene analytic method article calculation cell type cell viability celomocyte comet assay contamination controlled study DNA damage DNA repair earthworm environmental exposure environmental impact assessment natural red retention time nonhuman Animals Biological Markers Comet Assay Environmental Monitoring Oligochaeta Trinitrotoluene Eisenia fetida
description	Contamination by 2,4,6-trinitrotoluene (TNT) is a global environmental problem at sites of former explosive production, handling, or storage, and could have deleterious consequences for human and ecological health. We investigated its sublethal effects to Eisenia fetida, using two nonspecific biomarkers. In coelomocytes of earthworms exposed 24, 48, or 72 h, we evaluated DNA damage (comet assay) and neutral red retention time (NRRT), using the filter paper contact test. Both percentage of damage (D%) and calculated damage index showed significant DNA damage at almost all concentrations, at all time points assayed. Along exposure time, two different patterns were observed. At the lower TNT concentrations (0.25-0.5 μg/cm 2 ) an increased DNA migration at 48 h, with a decrease close to initial levels after 72 h exposure, was observed. This decrease could be attributed to activation of the DNA repair system. At higher concentrations (1.0-2.0 μg/cm 2 ), the high DNA damage observed remained constant during the 72 h exposure, suggesting that the rate of DNA repair was not enough to compensate such damage. Analysis of NRRT results showed a significant interaction between time and treatment. After 48 h, a significant decrease was observed at 4.0 μg/cm 2 . After 72 h, NRRT presented a concentration-dependent decrease, significantly different with respect to control at 0.5, 1.0, 2.0, and 4.0 μg/cm 2 . The two assayed methods, performed on the same sample, showed clear responses to sublethal TNT exposure in E. fetida, providing sensitive unspecific biomarkers of cell injury and DNA damage. © 2010 Springer Science+Business Media B.V.
format	JOUR
author	Fuchs, J. Piola, L. González, E.P. Oneto, M.L. Basack, S. Kesten, E. Casabé, N.
author_facet	Fuchs, J. Piola, L. González, E.P. Oneto, M.L. Basack, S. Kesten, E. Casabé, N.
author_sort	Fuchs, J.
title	Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)
title_short	Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)
title_full	Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)
title_fullStr	Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)
title_full_unstemmed	Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)
title_sort	coelomocyte biomarkers in the earthworm eisenia fetida exposed to 2,4,6-trinitrotoluene (tnt)
url	http://hdl.handle.net/20.500.12110/paper_01676369_v175_n1-4_p127_Fuchs
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_version_	1807316687891988480

Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)

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