Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas

Meningiomas, the most frequent benign intracranial and intraspinal types of tumors are normally removed by surgery. Complications can occur when the tumor is critically localized and cannot be completely removed or when comorbidities of the mostly elder patients increase the general surgical risk. T...

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Autores principales:	Curic, S., Wu, Y., Shan, B., Schaaf, C., Utpadel, D., Lange, M., Kuhlen, D., Perone, M.J., Arzt, E., Stalla, G.K., Renner, U.
Formato:	JOUR
Materias:	Apoptosis Curcumin G2/M phase cell cycle arrest Meningioma Proliferation caspase 3 curcumin phosphatidylserine thymidine antineoplastic agent adult aged antineoplastic activity antiproliferative activity apoptosis article cancer cell culture cell cycle arrest cell membrane cell transport concentration response confocal laser microscopy controlled study DNA fragmentation female flow cytometry fluorescence activated cell sorting G2 phase cell cycle checkpoint gene translocation human human cell human tissue immunoblotting immunofluorescence test in vitro study male meningioma Western blotting cell cycle cell proliferation drug effects fluorescent antibody technique metabolism pathology tumor cell culture Antineoplastic Agents Blotting, Western Cell Cycle Cell Proliferation Flow Cytometry Fluorescent Antibody Technique Humans Meningeal Neoplasms Tumor Cells, Cultured
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0167594X_v113_n3_p385_Curic
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0167594X_v113_n3_p385_Curic
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spelling	todo:paper_0167594X_v113_n3_p385_Curic2023-10-03T15:04:57Z Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas Curic, S. Wu, Y. Shan, B. Schaaf, C. Utpadel, D. Lange, M. Kuhlen, D. Perone, M.J. Arzt, E. Stalla, G.K. Renner, U. Apoptosis Curcumin G2/M phase cell cycle arrest Meningioma Proliferation caspase 3 curcumin phosphatidylserine thymidine antineoplastic agent curcumin adult aged antineoplastic activity antiproliferative activity apoptosis article cancer cell culture cell cycle arrest cell membrane cell transport concentration response confocal laser microscopy controlled study DNA fragmentation female flow cytometry fluorescence activated cell sorting G2 phase cell cycle checkpoint gene translocation human human cell human tissue immunoblotting immunofluorescence test in vitro study male meningioma Western blotting apoptosis cell cycle cell proliferation drug effects fluorescent antibody technique meningioma metabolism pathology tumor cell culture Antineoplastic Agents Apoptosis Blotting, Western Cell Cycle Cell Proliferation Curcumin Flow Cytometry Fluorescent Antibody Technique Humans Meningeal Neoplasms Meningioma Tumor Cells, Cultured Meningiomas, the most frequent benign intracranial and intraspinal types of tumors are normally removed by surgery. Complications can occur when the tumor is critically localized and cannot be completely removed or when comorbidities of the mostly elder patients increase the general surgical risk. Thus, alternate medical treatment concepts for the therapy of meningiomas would be desirable. Curcumin, the active ingredient of the spice plant Curcuma longa has shown anti-tumorigenic actions in many different types of tumors and therefore, its effect on growth and apoptosis of meningioma cells was studied in the present paper. In vitro, treatment of the human Ben-Men-1 meningioma cell line and of a series of 21 primary human meningioma cell cultures with curcumin (1-20 μM) strongly reduced the proliferation in all cases in a dose dependent manner. Cell cycle analysis by fluorescence-activated cell sorting showed growth arrest at G2/M phase, which was confirmed by demonstrating the corresponding modulation of proteins involved in G2/M arrest by immunoblotting and/or confocal laser microscopy. High dosages (20, 50 μM) of curcumin induced a significant increase of apoptosis in Ben-Men-1 and primary meningioma cell cultures as demonstrated by morphological changes of cell nuclei, DNA fragmentation, translocation of cell membrane associated phosphatidyl serine and the induction of apoptotic-acting cleaved caspase-3. Our results suggest that the multi-targeting drug curcumin has potent anti-tumorigenic actions in meningioma cells and might therefore be a putative candidate for the pharmacological treatment of meningiomas. © 2013 Springer Science+Business Media New York. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0167594X_v113_n3_p385_Curic
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apoptosis Curcumin G2/M phase cell cycle arrest Meningioma Proliferation caspase 3 curcumin phosphatidylserine thymidine antineoplastic agent curcumin adult aged antineoplastic activity antiproliferative activity apoptosis article cancer cell culture cell cycle arrest cell membrane cell transport concentration response confocal laser microscopy controlled study DNA fragmentation female flow cytometry fluorescence activated cell sorting G2 phase cell cycle checkpoint gene translocation human human cell human tissue immunoblotting immunofluorescence test in vitro study male meningioma Western blotting apoptosis cell cycle cell proliferation drug effects fluorescent antibody technique meningioma metabolism pathology tumor cell culture Antineoplastic Agents Apoptosis Blotting, Western Cell Cycle Cell Proliferation Curcumin Flow Cytometry Fluorescent Antibody Technique Humans Meningeal Neoplasms Meningioma Tumor Cells, Cultured
spellingShingle	Apoptosis Curcumin G2/M phase cell cycle arrest Meningioma Proliferation caspase 3 curcumin phosphatidylserine thymidine antineoplastic agent curcumin adult aged antineoplastic activity antiproliferative activity apoptosis article cancer cell culture cell cycle arrest cell membrane cell transport concentration response confocal laser microscopy controlled study DNA fragmentation female flow cytometry fluorescence activated cell sorting G2 phase cell cycle checkpoint gene translocation human human cell human tissue immunoblotting immunofluorescence test in vitro study male meningioma Western blotting apoptosis cell cycle cell proliferation drug effects fluorescent antibody technique meningioma metabolism pathology tumor cell culture Antineoplastic Agents Apoptosis Blotting, Western Cell Cycle Cell Proliferation Curcumin Flow Cytometry Fluorescent Antibody Technique Humans Meningeal Neoplasms Meningioma Tumor Cells, Cultured Curic, S. Wu, Y. Shan, B. Schaaf, C. Utpadel, D. Lange, M. Kuhlen, D. Perone, M.J. Arzt, E. Stalla, G.K. Renner, U. Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas
topic_facet	Apoptosis Curcumin G2/M phase cell cycle arrest Meningioma Proliferation caspase 3 curcumin phosphatidylserine thymidine antineoplastic agent curcumin adult aged antineoplastic activity antiproliferative activity apoptosis article cancer cell culture cell cycle arrest cell membrane cell transport concentration response confocal laser microscopy controlled study DNA fragmentation female flow cytometry fluorescence activated cell sorting G2 phase cell cycle checkpoint gene translocation human human cell human tissue immunoblotting immunofluorescence test in vitro study male meningioma Western blotting apoptosis cell cycle cell proliferation drug effects fluorescent antibody technique meningioma metabolism pathology tumor cell culture Antineoplastic Agents Apoptosis Blotting, Western Cell Cycle Cell Proliferation Curcumin Flow Cytometry Fluorescent Antibody Technique Humans Meningeal Neoplasms Meningioma Tumor Cells, Cultured
description	Meningiomas, the most frequent benign intracranial and intraspinal types of tumors are normally removed by surgery. Complications can occur when the tumor is critically localized and cannot be completely removed or when comorbidities of the mostly elder patients increase the general surgical risk. Thus, alternate medical treatment concepts for the therapy of meningiomas would be desirable. Curcumin, the active ingredient of the spice plant Curcuma longa has shown anti-tumorigenic actions in many different types of tumors and therefore, its effect on growth and apoptosis of meningioma cells was studied in the present paper. In vitro, treatment of the human Ben-Men-1 meningioma cell line and of a series of 21 primary human meningioma cell cultures with curcumin (1-20 μM) strongly reduced the proliferation in all cases in a dose dependent manner. Cell cycle analysis by fluorescence-activated cell sorting showed growth arrest at G2/M phase, which was confirmed by demonstrating the corresponding modulation of proteins involved in G2/M arrest by immunoblotting and/or confocal laser microscopy. High dosages (20, 50 μM) of curcumin induced a significant increase of apoptosis in Ben-Men-1 and primary meningioma cell cultures as demonstrated by morphological changes of cell nuclei, DNA fragmentation, translocation of cell membrane associated phosphatidyl serine and the induction of apoptotic-acting cleaved caspase-3. Our results suggest that the multi-targeting drug curcumin has potent anti-tumorigenic actions in meningioma cells and might therefore be a putative candidate for the pharmacological treatment of meningiomas. © 2013 Springer Science+Business Media New York.
format	JOUR
author	Curic, S. Wu, Y. Shan, B. Schaaf, C. Utpadel, D. Lange, M. Kuhlen, D. Perone, M.J. Arzt, E. Stalla, G.K. Renner, U.
author_facet	Curic, S. Wu, Y. Shan, B. Schaaf, C. Utpadel, D. Lange, M. Kuhlen, D. Perone, M.J. Arzt, E. Stalla, G.K. Renner, U.
author_sort	Curic, S.
title	Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas
title_short	Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas
title_full	Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas
title_fullStr	Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas
title_full_unstemmed	Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas
title_sort	curcumin acts anti-proliferative and pro-apoptotic in human meningiomas
url	http://hdl.handle.net/20.500.12110/paper_0167594X_v113_n3_p385_Curic
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_version_	1807323589012094976

Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas

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