Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia)
The molecular variants of Gonadotropin releasing hormone (GnRH) in brain extracts of the eutherian mammal Hydrochaeris hydrochaeris (Mammalia, Rodentia) were characterized. An indirect method combining reverse-phase high-performance liquid chromatography (RP-HPLC) and radioimmunoassay (RIA) with dif...
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todo:paper_01670115_v73_n3_p197_Montaner2023-10-03T15:04:31Z Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia) Montaner, A.D. Somoza, G.M. King, J.A. Bianchini, J.J. Bolis, C.G. Affanni, J.M. Capybara GnRH Gonadotropin-releasing hormone HPLC Mammalian Radioimmunoassay brain extract gonadorelin animal tissue article female forebrain hypothalamus male nonhuman olfactory bulb preoptic area priority journal reversed phase high performance liquid chromatography rodent Animals Chromatography, High Pressure Liquid Female Gonadotropin-Releasing Hormone Hypothalamus Male Mammals Olfactory Bulb Preoptic Area Prosencephalon Radioimmunoassay Rodentia Eutheria Gallus gallus Hydrochaeris hydrochaeris Mammalia Rodentia The molecular variants of Gonadotropin releasing hormone (GnRH) in brain extracts of the eutherian mammal Hydrochaeris hydrochaeris (Mammalia, Rodentia) were characterized. An indirect method combining reverse-phase high-performance liquid chromatography (RP-HPLC) and radioimmunoassay (RIA) with different antisera was used. Two different forebrain regions (olfactory bulbs and preoptic-hypothalamic region) were analyzed. Characterization of RP-HPLC fractions from preoptic-hypothalamic extracts with three different RIA systems revealed two immunoreactive GnRH (ir-GnRH) peaks coeluting with mammalian GnRH (mGnRH) and salmon GnRH (sGnRH) synthetic standards. These results were additionally supported by serial dilution studies with specific antisera. Similar results were obtained from olfactory bulb extracts with the same methodology. However, a third ir-GnRH peak in a similar position to that of chicken GnRH II (cIIGnRH) synthetic standard was revealed. As far as we know, this is the first report showing chromatographic and immunological evidences for the presence of a second GnRH variant in the forebrain of an eutherian mammal. Copyright (C) 1998 Elsevier Science B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01670115_v73_n3_p197_Montaner |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Capybara GnRH Gonadotropin-releasing hormone HPLC Mammalian Radioimmunoassay brain extract gonadorelin animal tissue article female forebrain hypothalamus male nonhuman olfactory bulb preoptic area priority journal reversed phase high performance liquid chromatography rodent Animals Chromatography, High Pressure Liquid Female Gonadotropin-Releasing Hormone Hypothalamus Male Mammals Olfactory Bulb Preoptic Area Prosencephalon Radioimmunoassay Rodentia Eutheria Gallus gallus Hydrochaeris hydrochaeris Mammalia Rodentia |
| spellingShingle |
Capybara GnRH Gonadotropin-releasing hormone HPLC Mammalian Radioimmunoassay brain extract gonadorelin animal tissue article female forebrain hypothalamus male nonhuman olfactory bulb preoptic area priority journal reversed phase high performance liquid chromatography rodent Animals Chromatography, High Pressure Liquid Female Gonadotropin-Releasing Hormone Hypothalamus Male Mammals Olfactory Bulb Preoptic Area Prosencephalon Radioimmunoassay Rodentia Eutheria Gallus gallus Hydrochaeris hydrochaeris Mammalia Rodentia Montaner, A.D. Somoza, G.M. King, J.A. Bianchini, J.J. Bolis, C.G. Affanni, J.M. Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia) |
| topic_facet |
Capybara GnRH Gonadotropin-releasing hormone HPLC Mammalian Radioimmunoassay brain extract gonadorelin animal tissue article female forebrain hypothalamus male nonhuman olfactory bulb preoptic area priority journal reversed phase high performance liquid chromatography rodent Animals Chromatography, High Pressure Liquid Female Gonadotropin-Releasing Hormone Hypothalamus Male Mammals Olfactory Bulb Preoptic Area Prosencephalon Radioimmunoassay Rodentia Eutheria Gallus gallus Hydrochaeris hydrochaeris Mammalia Rodentia |
| description |
The molecular variants of Gonadotropin releasing hormone (GnRH) in brain extracts of the eutherian mammal Hydrochaeris hydrochaeris (Mammalia, Rodentia) were characterized. An indirect method combining reverse-phase high-performance liquid chromatography (RP-HPLC) and radioimmunoassay (RIA) with different antisera was used. Two different forebrain regions (olfactory bulbs and preoptic-hypothalamic region) were analyzed. Characterization of RP-HPLC fractions from preoptic-hypothalamic extracts with three different RIA systems revealed two immunoreactive GnRH (ir-GnRH) peaks coeluting with mammalian GnRH (mGnRH) and salmon GnRH (sGnRH) synthetic standards. These results were additionally supported by serial dilution studies with specific antisera. Similar results were obtained from olfactory bulb extracts with the same methodology. However, a third ir-GnRH peak in a similar position to that of chicken GnRH II (cIIGnRH) synthetic standard was revealed. As far as we know, this is the first report showing chromatographic and immunological evidences for the presence of a second GnRH variant in the forebrain of an eutherian mammal. Copyright (C) 1998 Elsevier Science B.V. |
| format |
JOUR |
| author |
Montaner, A.D. Somoza, G.M. King, J.A. Bianchini, J.J. Bolis, C.G. Affanni, J.M. |
| author_facet |
Montaner, A.D. Somoza, G.M. King, J.A. Bianchini, J.J. Bolis, C.G. Affanni, J.M. |
| author_sort |
Montaner, A.D. |
| title |
Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia) |
| title_short |
Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia) |
| title_full |
Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia) |
| title_fullStr |
Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia) |
| title_full_unstemmed |
Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia) |
| title_sort |
chromatographic and immunological identification of gnrh (gonadotropin-releasing hormone) variants. occurrence of mammalian and a salmon-like gnrh in the forebrain of an eutherian mammal: hydrochaeris hydrochaeris (mammalia, rodentia) |
| url |
http://hdl.handle.net/20.500.12110/paper_01670115_v73_n3_p197_Montaner |
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