The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units

The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [ 14 C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding t...

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Autores principales:	Couto, A.S., Goncalves, M.F., Colli, W., De Lederkremer, R.M.
Formato:	JOUR
Materias:	Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Animalia Protozoa Triticum aestivum Trypanosoma
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01666851_v39_n1_p101_Couto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01666851_v39_n1_p101_Couto
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spelling	todo:paper_01666851_v39_n1_p101_Couto2023-10-03T15:04:19Z The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units Couto, A.S. Goncalves, M.F. Colli, W. De Lederkremer, R.M. Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Protozoa Triticum aestivum Trypanosoma Trypanosoma cruzi The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [ 14 C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding to the lectin was lost on treatment of Tc-85 with neuraminidase. The N-linked asialo-oligosaccharide was released by endo-β-N-acetylglucosaminidase F digestion of asialo-Tc-85 and was further analyzed using specific exoglycosidases. [ 14 C]fucose was detected after α-l-fucosidase treatment or mild acid hydrolysis. The afucosyl oligosaccharide was 3 H-labeled by the galactose oxidase-NaB 3 H 4 method. [ 3 H]Galactose was released by α-galactosidase, and only then was β-galactosidase effective in removing another galactose. The gal(α1-3)gal unit was demonstrated by periodate oxidation studies on the [ 3 H]galactose-labeled asialo-glycoprotein. The presence of gal(α1-3)gal in Tc-85 could be related to the recent finding of elevated antibody levels against this epitope in patients with Chagas' disease. © 1990. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v39_n1_p101_Couto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Protozoa Triticum aestivum Trypanosoma Trypanosoma cruzi
spellingShingle	Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Protozoa Triticum aestivum Trypanosoma Trypanosoma cruzi Couto, A.S. Goncalves, M.F. Colli, W. De Lederkremer, R.M. The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
topic_facet	Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Protozoa Triticum aestivum Trypanosoma Trypanosoma cruzi
description	The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [ 14 C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding to the lectin was lost on treatment of Tc-85 with neuraminidase. The N-linked asialo-oligosaccharide was released by endo-β-N-acetylglucosaminidase F digestion of asialo-Tc-85 and was further analyzed using specific exoglycosidases. [ 14 C]fucose was detected after α-l-fucosidase treatment or mild acid hydrolysis. The afucosyl oligosaccharide was 3 H-labeled by the galactose oxidase-NaB 3 H 4 method. [ 3 H]Galactose was released by α-galactosidase, and only then was β-galactosidase effective in removing another galactose. The gal(α1-3)gal unit was demonstrated by periodate oxidation studies on the [ 3 H]galactose-labeled asialo-glycoprotein. The presence of gal(α1-3)gal in Tc-85 could be related to the recent finding of elevated antibody levels against this epitope in patients with Chagas' disease. © 1990.
format	JOUR
author	Couto, A.S. Goncalves, M.F. Colli, W. De Lederkremer, R.M.
author_facet	Couto, A.S. Goncalves, M.F. Colli, W. De Lederkremer, R.M.
author_sort	Couto, A.S.
title	The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
title_short	The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
title_full	The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
title_fullStr	The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
title_full_unstemmed	The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
title_sort	n-linked carbohydrate chain of the 85-kilodalton glycoprotein from trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
url	http://hdl.handle.net/20.500.12110/paper_01666851_v39_n1_p101_Couto
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The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units

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