Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues
The size of a lysosomal cysteine proteinase from epimastigotes of Trypanosoma cruzi decreased from 60 to 54 kDa upon treatment with endo β N acetylglucosaminidase H. A lower-molecular weight component (30-35 kDa), which usually accompanies the 60-kDa protein also increased its electrophoretic mobili...
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todo:paper_01666851_v38_n1_p41_Cazzulo2023-10-03T15:04:19Z Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues Cazzulo, J.J. Hellman, U. Couso, R. Parodi, A.J.A. Amino acid composition Cysteine proteinase Lysosomal enzyme Oligosaccharide composition Trypanosoma cruzi amino acid carbohydrate cysteine proteinase mannose article lysosome nonhuman priority journal protozoon trypanosoma cruzi Amino Acid Sequence Amino Acids Animal Carbohydrate Sequence Carbohydrates Cysteine Endopeptidases Lysosomes Mannosephosphates Molecular Sequence Data Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi The size of a lysosomal cysteine proteinase from epimastigotes of Trypanosoma cruzi decreased from 60 to 54 kDa upon treatment with endo β N acetylglucosaminidase H. A lower-molecular weight component (30-35 kDa), which usually accompanies the 60-kDa protein also increased its electrophoretic mobility, and seems to consist of a mixture of degradation products of the enzyme, since both the larger and the smaller components had the same N-terminal sequence as the 60-kDa protein. The amino acid composition of the protein moiety and the composition of the oligosaccharide chains have been determined. The oligosaccharide chains are of the high-mannose type, and contain 6, 7, 8 or 9 mannose residues, as shown both by in vivo labelling with [U-14C]glucose, and by labeling the endo β N acetylglucosaminidase H-sensitive oligosaccharides of the purified enzyme with tritiated sodium borohydride. The oligosaccharide chains did not contain phosphate residues. Further studies with [U-14C]-labeled total glycoproteins of T. cruzi, and enzyme assays, suggest that T. cruzi and other trypanosomatids do not target their lysosomal enzymes to the organelle through the mannose-6-phosphate marker pathway. © 1990. Fil:Couso, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parodi, A.J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v38_n1_p41_Cazzulo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amino acid composition Cysteine proteinase Lysosomal enzyme Oligosaccharide composition Trypanosoma cruzi amino acid carbohydrate cysteine proteinase mannose article lysosome nonhuman priority journal protozoon trypanosoma cruzi Amino Acid Sequence Amino Acids Animal Carbohydrate Sequence Carbohydrates Cysteine Endopeptidases Lysosomes Mannosephosphates Molecular Sequence Data Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi |
| spellingShingle |
Amino acid composition Cysteine proteinase Lysosomal enzyme Oligosaccharide composition Trypanosoma cruzi amino acid carbohydrate cysteine proteinase mannose article lysosome nonhuman priority journal protozoon trypanosoma cruzi Amino Acid Sequence Amino Acids Animal Carbohydrate Sequence Carbohydrates Cysteine Endopeptidases Lysosomes Mannosephosphates Molecular Sequence Data Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi Cazzulo, J.J. Hellman, U. Couso, R. Parodi, A.J.A. Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues |
| topic_facet |
Amino acid composition Cysteine proteinase Lysosomal enzyme Oligosaccharide composition Trypanosoma cruzi amino acid carbohydrate cysteine proteinase mannose article lysosome nonhuman priority journal protozoon trypanosoma cruzi Amino Acid Sequence Amino Acids Animal Carbohydrate Sequence Carbohydrates Cysteine Endopeptidases Lysosomes Mannosephosphates Molecular Sequence Data Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi |
| description |
The size of a lysosomal cysteine proteinase from epimastigotes of Trypanosoma cruzi decreased from 60 to 54 kDa upon treatment with endo β N acetylglucosaminidase H. A lower-molecular weight component (30-35 kDa), which usually accompanies the 60-kDa protein also increased its electrophoretic mobility, and seems to consist of a mixture of degradation products of the enzyme, since both the larger and the smaller components had the same N-terminal sequence as the 60-kDa protein. The amino acid composition of the protein moiety and the composition of the oligosaccharide chains have been determined. The oligosaccharide chains are of the high-mannose type, and contain 6, 7, 8 or 9 mannose residues, as shown both by in vivo labelling with [U-14C]glucose, and by labeling the endo β N acetylglucosaminidase H-sensitive oligosaccharides of the purified enzyme with tritiated sodium borohydride. The oligosaccharide chains did not contain phosphate residues. Further studies with [U-14C]-labeled total glycoproteins of T. cruzi, and enzyme assays, suggest that T. cruzi and other trypanosomatids do not target their lysosomal enzymes to the organelle through the mannose-6-phosphate marker pathway. © 1990. |
| format |
JOUR |
| author |
Cazzulo, J.J. Hellman, U. Couso, R. Parodi, A.J.A. |
| author_facet |
Cazzulo, J.J. Hellman, U. Couso, R. Parodi, A.J.A. |
| author_sort |
Cazzulo, J.J. |
| title |
Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues |
| title_short |
Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues |
| title_full |
Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues |
| title_fullStr |
Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues |
| title_full_unstemmed |
Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues |
| title_sort |
amino acid and carbohydrate composition of a lysosomal cysteine proteinase from trypanosoma cruzi. absence of phosphorylated mannose residues |
| url |
http://hdl.handle.net/20.500.12110/paper_01666851_v38_n1_p41_Cazzulo |
| work_keys_str_mv |
AT cazzulojj aminoacidandcarbohydratecompositionofalysosomalcysteineproteinasefromtrypanosomacruziabsenceofphosphorylatedmannoseresidues AT hellmanu aminoacidandcarbohydratecompositionofalysosomalcysteineproteinasefromtrypanosomacruziabsenceofphosphorylatedmannoseresidues AT cousor aminoacidandcarbohydratecompositionofalysosomalcysteineproteinasefromtrypanosomacruziabsenceofphosphorylatedmannoseresidues AT parodiaja aminoacidandcarbohydratecompositionofalysosomalcysteineproteinasefromtrypanosomacruziabsenceofphosphorylatedmannoseresidues |
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