Identification of core components of the exon junction complex in trypanosomes
In animal cells, the exon junction complex (EJC) is deposited onto mRNAs during the second step of splicing, 20-24 nt upstream of the exon-exon junction. The EJC core contains four proteins: Mago, Y14, eIF4AIII and Btz. In trypanosomes, cis-splicing is very rare but all mRNAs are subject to 5′ trans...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01666851_v166_n2_p190_Bercovich |
| Aporte de: |
| id |
todo:paper_01666851_v166_n2_p190_Bercovich |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01666851_v166_n2_p190_Bercovich2023-10-03T15:04:15Z Identification of core components of the exon junction complex in trypanosomes Bercovich, N. Levin, M.J. Clayton, C. Vazquez, M.P. EJC Mago mRNA export Trypanosomes Y14 heterodimer messenger RNA protozoal protein article evolutionary homology exon in vivo study nonhuman nucleotide sequence priority journal protein domain RNA sequence RNA splicing trans splicing Trypanosoma yeast Amino Acid Sequence Animals Exons Humans Molecular Sequence Data Protein Binding Protozoan Proteins RNA Splicing RNA Transport RNA-Binding Proteins Sequence Alignment Trypanosoma brucei brucei Trypanosoma cruzi Trypanosomiasis Animalia Mago In animal cells, the exon junction complex (EJC) is deposited onto mRNAs during the second step of splicing, 20-24 nt upstream of the exon-exon junction. The EJC core contains four proteins: Mago, Y14, eIF4AIII and Btz. In trypanosomes, cis-splicing is very rare but all mRNAs are subject to 5′ trans-splicing of a 39-nt RNA sequence. Here we show that trypanosomes have a conserved Mago and a divergent Y14 protein, but we were unable to identify a Btz orthologue. We demonstrate that Mago and Y14 form a stable heterodimer using yeast two hybrid analyses. We also show that this complex co-purifies in vivo in trypanosomes with a protein containing an NTF2 domain, typically involved in mRNA transport. © 2009 Elsevier B.V. All rights reserved. Fil:Bercovich, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v166_n2_p190_Bercovich |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
EJC Mago mRNA export Trypanosomes Y14 heterodimer messenger RNA protozoal protein article evolutionary homology exon in vivo study nonhuman nucleotide sequence priority journal protein domain RNA sequence RNA splicing trans splicing Trypanosoma yeast Amino Acid Sequence Animals Exons Humans Molecular Sequence Data Protein Binding Protozoan Proteins RNA Splicing RNA Transport RNA-Binding Proteins Sequence Alignment Trypanosoma brucei brucei Trypanosoma cruzi Trypanosomiasis Animalia Mago |
| spellingShingle |
EJC Mago mRNA export Trypanosomes Y14 heterodimer messenger RNA protozoal protein article evolutionary homology exon in vivo study nonhuman nucleotide sequence priority journal protein domain RNA sequence RNA splicing trans splicing Trypanosoma yeast Amino Acid Sequence Animals Exons Humans Molecular Sequence Data Protein Binding Protozoan Proteins RNA Splicing RNA Transport RNA-Binding Proteins Sequence Alignment Trypanosoma brucei brucei Trypanosoma cruzi Trypanosomiasis Animalia Mago Bercovich, N. Levin, M.J. Clayton, C. Vazquez, M.P. Identification of core components of the exon junction complex in trypanosomes |
| topic_facet |
EJC Mago mRNA export Trypanosomes Y14 heterodimer messenger RNA protozoal protein article evolutionary homology exon in vivo study nonhuman nucleotide sequence priority journal protein domain RNA sequence RNA splicing trans splicing Trypanosoma yeast Amino Acid Sequence Animals Exons Humans Molecular Sequence Data Protein Binding Protozoan Proteins RNA Splicing RNA Transport RNA-Binding Proteins Sequence Alignment Trypanosoma brucei brucei Trypanosoma cruzi Trypanosomiasis Animalia Mago |
| description |
In animal cells, the exon junction complex (EJC) is deposited onto mRNAs during the second step of splicing, 20-24 nt upstream of the exon-exon junction. The EJC core contains four proteins: Mago, Y14, eIF4AIII and Btz. In trypanosomes, cis-splicing is very rare but all mRNAs are subject to 5′ trans-splicing of a 39-nt RNA sequence. Here we show that trypanosomes have a conserved Mago and a divergent Y14 protein, but we were unable to identify a Btz orthologue. We demonstrate that Mago and Y14 form a stable heterodimer using yeast two hybrid analyses. We also show that this complex co-purifies in vivo in trypanosomes with a protein containing an NTF2 domain, typically involved in mRNA transport. © 2009 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Bercovich, N. Levin, M.J. Clayton, C. Vazquez, M.P. |
| author_facet |
Bercovich, N. Levin, M.J. Clayton, C. Vazquez, M.P. |
| author_sort |
Bercovich, N. |
| title |
Identification of core components of the exon junction complex in trypanosomes |
| title_short |
Identification of core components of the exon junction complex in trypanosomes |
| title_full |
Identification of core components of the exon junction complex in trypanosomes |
| title_fullStr |
Identification of core components of the exon junction complex in trypanosomes |
| title_full_unstemmed |
Identification of core components of the exon junction complex in trypanosomes |
| title_sort |
identification of core components of the exon junction complex in trypanosomes |
| url |
http://hdl.handle.net/20.500.12110/paper_01666851_v166_n2_p190_Bercovich |
| work_keys_str_mv |
AT bercovichn identificationofcorecomponentsoftheexonjunctioncomplexintrypanosomes AT levinmj identificationofcorecomponentsoftheexonjunctioncomplexintrypanosomes AT claytonc identificationofcorecomponentsoftheexonjunctioncomplexintrypanosomes AT vazquezmp identificationofcorecomponentsoftheexonjunctioncomplexintrypanosomes |
| _version_ |
1807323171089547264 |