Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosp...
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todo:paper_01666851_v158_n2_p120_Salto2023-10-03T15:04:14Z Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi Salto, M.L. Kuhlenschmidt, T. Kuhlenschmidt, M. de Lederkremer, R.M. Docampo, R. Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Trypanosoma cruzi Animals Calcium Centrifugation, Density Gradient Glycolipids Hydrogen-Ion Concentration Organelles Phospholipids Spectrometry, Mass, Electrospray Ionization Trypanosoma cruzi Trypanosoma cruzi Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [3H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition. © 2007 Elsevier B.V. All rights reserved. Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v158_n2_p120_Salto |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Trypanosoma cruzi Animals Calcium Centrifugation, Density Gradient Glycolipids Hydrogen-Ion Concentration Organelles Phospholipids Spectrometry, Mass, Electrospray Ionization Trypanosoma cruzi Trypanosoma cruzi |
| spellingShingle |
Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Trypanosoma cruzi Animals Calcium Centrifugation, Density Gradient Glycolipids Hydrogen-Ion Concentration Organelles Phospholipids Spectrometry, Mass, Electrospray Ionization Trypanosoma cruzi Trypanosoma cruzi Salto, M.L. Kuhlenschmidt, T. Kuhlenschmidt, M. de Lederkremer, R.M. Docampo, R. Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi |
| topic_facet |
Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Trypanosoma cruzi Animals Calcium Centrifugation, Density Gradient Glycolipids Hydrogen-Ion Concentration Organelles Phospholipids Spectrometry, Mass, Electrospray Ionization Trypanosoma cruzi Trypanosoma cruzi |
| description |
Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [3H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition. © 2007 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Salto, M.L. Kuhlenschmidt, T. Kuhlenschmidt, M. de Lederkremer, R.M. Docampo, R. |
| author_facet |
Salto, M.L. Kuhlenschmidt, T. Kuhlenschmidt, M. de Lederkremer, R.M. Docampo, R. |
| author_sort |
Salto, M.L. |
| title |
Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi |
| title_short |
Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi |
| title_full |
Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi |
| title_fullStr |
Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi |
| title_full_unstemmed |
Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi |
| title_sort |
phospholipid and glycolipid composition of acidocalcisomes of trypanosoma cruzi |
| url |
http://hdl.handle.net/20.500.12110/paper_01666851_v158_n2_p120_Salto |
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