Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant

The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in...

Descripción completa

Detalles Bibliográficos
Autores principales:	Muñoz, M.J., Santori, M.I., Rojas, F., Gómez, E.B., Téllez-Iñón, M.T.
Formato:	JOUR
Materias:	Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces pombe Proteins Sequence Analysis, DNA
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01666851_v147_n2_p154_Munoz
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01666851_v147_n2_p154_Munoz
record_format	dspace
spelling	todo:paper_01666851_v147_n2_p154_Munoz2023-10-03T15:04:13Z Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant Muñoz, M.J. Santori, M.I. Rojas, F. Gómez, E.B. Téllez-Iñón, M.T. Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan protein kinase Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe Trypanosoma cruzi trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma cruzi Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Trypanosoma cruzi The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in yeast and their action has been implicated in the regulation of CDK function. In the present work we identified Tcp12CKS1 a member of the CKS family in the parasite Trypanosoma cruzi. TcCKS1 is expressed in the three forms of T. cruzi. By using anti-Tcp12CKS1 antiserum, protein kinase (PK) activities were immunoprecipitated. The PK activity level varies depending on the stage analyzed, being lower in trypomastigotes and thus suggesting that different stages have different CKS-CRK complexes. Moreover, these PK activities were inhibited by using Flavopiridol, a known CDKs inhibitor. Western blot analyses demonstrated that in the epimastigote stage, p12CKS1 stably interacts with TcCRK1 and TcCRK3. In addition, Tcp12CKS1 was able to rescue the p13SUC1 null mutant of S. pombe. The functional complementation between the CKS proteins of two evolutionary distant organisms supports the role of Tcp12CKS1 as a key regulator in T. cruzi cell cycle. © 2006 Elsevier B.V. All rights reserved. Fil:Muñoz, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Santori, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rojas, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gómez, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v147_n2_p154_Munoz
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan protein kinase Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe Trypanosoma cruzi trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma cruzi Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Trypanosoma cruzi
spellingShingle	Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan protein kinase Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe Trypanosoma cruzi trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma cruzi Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Trypanosoma cruzi Muñoz, M.J. Santori, M.I. Rojas, F. Gómez, E.B. Téllez-Iñón, M.T. Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
topic_facet	Cell cycle CKS CRKs Cyclins Trypanosoma cruzi antiserum cyclin dependent kinase cyclin dependent kinase 1 cyclin dependent kinase inhibitor cycline protein kinase protozoal protein Schizosaccharomyces pombe protein cell cycle protein CRK protein, protozoan protein kinase Suc1 protein, S pombe article cell cycle enzyme activity enzyme inhibition epimastigote immunoprecipitation nonhuman nucleotide sequence priority journal protein analysis protein interaction Schizosaccharomyces pombe Trypanosoma cruzi trypomastigote Western blotting yeast amino acid sequence animal chemistry DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics mutation Schizosaccharomyces Schizosaccharomyces pombe Trypanosoma cruzi Trypanosomatidae Amino Acid Sequence Animals CDC2 Protein Kinase Cell Cycle Proteins Gene Expression Regulation Molecular Sequence Data Mutation Protein Kinases Protozoan Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Trypanosoma cruzi
description	The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in yeast and their action has been implicated in the regulation of CDK function. In the present work we identified Tcp12CKS1 a member of the CKS family in the parasite Trypanosoma cruzi. TcCKS1 is expressed in the three forms of T. cruzi. By using anti-Tcp12CKS1 antiserum, protein kinase (PK) activities were immunoprecipitated. The PK activity level varies depending on the stage analyzed, being lower in trypomastigotes and thus suggesting that different stages have different CKS-CRK complexes. Moreover, these PK activities were inhibited by using Flavopiridol, a known CDKs inhibitor. Western blot analyses demonstrated that in the epimastigote stage, p12CKS1 stably interacts with TcCRK1 and TcCRK3. In addition, Tcp12CKS1 was able to rescue the p13SUC1 null mutant of S. pombe. The functional complementation between the CKS proteins of two evolutionary distant organisms supports the role of Tcp12CKS1 as a key regulator in T. cruzi cell cycle. © 2006 Elsevier B.V. All rights reserved.
format	JOUR
author	Muñoz, M.J. Santori, M.I. Rojas, F. Gómez, E.B. Téllez-Iñón, M.T.
author_facet	Muñoz, M.J. Santori, M.I. Rojas, F. Gómez, E.B. Téllez-Iñón, M.T.
author_sort	Muñoz, M.J.
title	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_short	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_full	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_fullStr	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_full_unstemmed	Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
title_sort	trypanosoma cruzi tcp12cks1 interacts with parasite crks and rescues the p13suc1 fission yeast mutant
url	http://hdl.handle.net/20.500.12110/paper_01666851_v147_n2_p154_Munoz
work_keys_str_mv	AT munozmj trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant AT santorimi trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant AT rojasf trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant AT gomezeb trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant AT tellezinonmt trypanosomacruzitcp12cks1interactswithparasitecrksandrescuesthep13suc1fissionyeastmutant
_version_	1807321856784465920

Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant

Ejemplares similares