Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi

Gene expression in trypanosomes is controlled mainly by post-transcriptional processes. This study was designed to analyse HX1, one of the TcP2β upstream intergenic regions. It is an efficient pre-mRNA processing region that has been widely and successfully used in Trypanosoma cruzi transfection vec...

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Autores principales: Ben-Dov, C.P., Levin, M.J., Vázquez, M.P.
Formato: JOUR
Materias:
Chagas disease
Post-transcriptional regulation
Repetitive sequences
trans-Splicing
messenger RNA
ribosome protein
5' untranslated region
article
gene locus
mutational analysis
nonhuman
priority journal
protein analysis
protein synthesis
RNA processing
trans splicing
transcription regulation
Trypanosoma cruzi
insertion sequences
Trypanosoma
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01666851_v140_n1_p97_BenDov
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01666851_v140_n1_p97_BenDov
record_format dspace
spelling todo:paper_01666851_v140_n1_p97_BenDov2023-10-03T15:04:11Z Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi Ben-Dov, C.P. Levin, M.J. Vázquez, M.P. Chagas disease Post-transcriptional regulation Repetitive sequences trans-Splicing messenger RNA ribosome protein 5' untranslated region article gene locus mutational analysis nonhuman priority journal protein analysis protein synthesis RNA processing trans splicing transcription regulation Trypanosoma cruzi insertion sequences Trypanosoma Trypanosoma cruzi Trypanosoma cruzi Gene expression in trypanosomes is controlled mainly by post-transcriptional processes. This study was designed to analyse HX1, one of the TcP2β upstream intergenic regions. It is an efficient pre-mRNA processing region that has been widely and successfully used in Trypanosoma cruzi transfection vectors. Herein we compared its performance with other regions within the same locus, and we identified the sequence elements responsible for the HX1 efficiency in trans-splicing and protein synthesis. Our mutational analysis showed the flexibility of the branch point site selection for HX1 trans-splicing process. We demonstrated also that its 12 nt 5′UTR sequence contributes to both trans-splicing and translation efficiency. The natural insertion of the repetitive element short interspersed repetitive element (SIRE) in one of the HX1 polypyrimidine tracts decreases the translated protein level by 40%. In this report, we demonstrated that this reduction is a consequence of a decrease of five-fold in the level of processed mRNA balanced by an increased efficiency of translation due to the inclusion of a 38 nt SIRE specific sequence in the 5′UTR of the mRNA. © 2005 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v140_n1_p97_BenDov
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Chagas disease
Post-transcriptional regulation
Repetitive sequences
trans-Splicing
messenger RNA
ribosome protein
5' untranslated region
article
gene locus
mutational analysis
nonhuman
priority journal
protein analysis
protein synthesis
RNA processing
trans splicing
transcription regulation
Trypanosoma cruzi
insertion sequences
Trypanosoma
Trypanosoma cruzi
Trypanosoma cruzi
spellingShingle Chagas disease
Post-transcriptional regulation
Repetitive sequences
trans-Splicing
messenger RNA
ribosome protein
5' untranslated region
article
gene locus
mutational analysis
nonhuman
priority journal
protein analysis
protein synthesis
RNA processing
trans splicing
transcription regulation
Trypanosoma cruzi
insertion sequences
Trypanosoma
Trypanosoma cruzi
Trypanosoma cruzi
Ben-Dov, C.P.
Levin, M.J.
Vázquez, M.P.
Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi
topic_facet Chagas disease
Post-transcriptional regulation
Repetitive sequences
trans-Splicing
messenger RNA
ribosome protein
5' untranslated region
article
gene locus
mutational analysis
nonhuman
priority journal
protein analysis
protein synthesis
RNA processing
trans splicing
transcription regulation
Trypanosoma cruzi
insertion sequences
Trypanosoma
Trypanosoma cruzi
Trypanosoma cruzi
description Gene expression in trypanosomes is controlled mainly by post-transcriptional processes. This study was designed to analyse HX1, one of the TcP2β upstream intergenic regions. It is an efficient pre-mRNA processing region that has been widely and successfully used in Trypanosoma cruzi transfection vectors. Herein we compared its performance with other regions within the same locus, and we identified the sequence elements responsible for the HX1 efficiency in trans-splicing and protein synthesis. Our mutational analysis showed the flexibility of the branch point site selection for HX1 trans-splicing process. We demonstrated also that its 12 nt 5′UTR sequence contributes to both trans-splicing and translation efficiency. The natural insertion of the repetitive element short interspersed repetitive element (SIRE) in one of the HX1 polypyrimidine tracts decreases the translated protein level by 40%. In this report, we demonstrated that this reduction is a consequence of a decrease of five-fold in the level of processed mRNA balanced by an increased efficiency of translation due to the inclusion of a 38 nt SIRE specific sequence in the 5′UTR of the mRNA. © 2005 Elsevier B.V. All rights reserved.
format JOUR
author Ben-Dov, C.P.
Levin, M.J.
Vázquez, M.P.
author_facet Ben-Dov, C.P.
Levin, M.J.
Vázquez, M.P.
author_sort Ben-Dov, C.P.
title Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi
title_short Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi
title_full Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi
title_fullStr Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi
title_full_unstemmed Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi
title_sort analysis of the highly efficient pre-mrna processing region hx1 of trypanosoma cruzi
url http://hdl.handle.net/20.500.12110/paper_01666851_v140_n1_p97_BenDov
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AT levinmj analysisofthehighlyefficientpremrnaprocessingregionhx1oftrypanosomacruzi
AT vazquezmp analysisofthehighlyefficientpremrnaprocessingregionhx1oftrypanosomacruzi
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