Azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus
Azinphos-methyl (AZM) and chlorpyrifos (CPF) are broad-spectrum organophosphate insecticides used for pest control on a number of food crops in many parts of the world that have been shown to inhibit cholinesterase activity in the non-target freshwater gastropod Planorbarius corneus. The present stu...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p12_Cacciatore |
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todo:paper_0166445X_v167_n_p12_Cacciatore |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antioxidant enzymes Invertebrates Mixtures Organophosphates Pesticides azinphos methyl catalase chlorpyrifos glucose 6 phosphate dehydrogenase glutathione glutathione disulfide glutathione reductase glutathione transferase malonaldehyde polyunsaturated fatty acid reactive oxygen metabolite superoxide dismutase azinphos methyl catalase chlorpyrifos fresh water glutathione reductase glutathione transferase insecticide water pollutant azinphos methyl chemical pollutant chlorpyrifos enzyme activity gastropod physiological response redox conditions toxicity adult animal experiment animal tissue Article comparative study concentration (parameters) controlled study environmental exposure enzyme activity freshwater species gastropod lipid peroxidation molecular dynamics neurotoxicity nonhuman oxidation reduction state oxidative stress Planorbarius corneus priority journal protein content protein expression protein localization tissue distribution upregulation animal drug effects enzyme activation gastropod lipid peroxidation metabolism oxidative stress toxicity water pollutant Animalia Gastropoda Invertebrata Planorbarius corneus Animals Azinphosmethyl Catalase Chlorpyrifos Enzyme Activation Fresh Water Gastropoda Glutathione Reductase Glutathione Transferase Insecticides Lipid Peroxidation Oxidative Stress Water Pollutants, Chemical |
| spellingShingle |
Antioxidant enzymes Invertebrates Mixtures Organophosphates Pesticides azinphos methyl catalase chlorpyrifos glucose 6 phosphate dehydrogenase glutathione glutathione disulfide glutathione reductase glutathione transferase malonaldehyde polyunsaturated fatty acid reactive oxygen metabolite superoxide dismutase azinphos methyl catalase chlorpyrifos fresh water glutathione reductase glutathione transferase insecticide water pollutant azinphos methyl chemical pollutant chlorpyrifos enzyme activity gastropod physiological response redox conditions toxicity adult animal experiment animal tissue Article comparative study concentration (parameters) controlled study environmental exposure enzyme activity freshwater species gastropod lipid peroxidation molecular dynamics neurotoxicity nonhuman oxidation reduction state oxidative stress Planorbarius corneus priority journal protein content protein expression protein localization tissue distribution upregulation animal drug effects enzyme activation gastropod lipid peroxidation metabolism oxidative stress toxicity water pollutant Animalia Gastropoda Invertebrata Planorbarius corneus Animals Azinphosmethyl Catalase Chlorpyrifos Enzyme Activation Fresh Water Gastropoda Glutathione Reductase Glutathione Transferase Insecticides Lipid Peroxidation Oxidative Stress Water Pollutants, Chemical Cacciatore, L.C. Nemirovsky, S.I. Verrengia Guerrero, N.R. Cochón, A.C. Azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus |
| topic_facet |
Antioxidant enzymes Invertebrates Mixtures Organophosphates Pesticides azinphos methyl catalase chlorpyrifos glucose 6 phosphate dehydrogenase glutathione glutathione disulfide glutathione reductase glutathione transferase malonaldehyde polyunsaturated fatty acid reactive oxygen metabolite superoxide dismutase azinphos methyl catalase chlorpyrifos fresh water glutathione reductase glutathione transferase insecticide water pollutant azinphos methyl chemical pollutant chlorpyrifos enzyme activity gastropod physiological response redox conditions toxicity adult animal experiment animal tissue Article comparative study concentration (parameters) controlled study environmental exposure enzyme activity freshwater species gastropod lipid peroxidation molecular dynamics neurotoxicity nonhuman oxidation reduction state oxidative stress Planorbarius corneus priority journal protein content protein expression protein localization tissue distribution upregulation animal drug effects enzyme activation gastropod lipid peroxidation metabolism oxidative stress toxicity water pollutant Animalia Gastropoda Invertebrata Planorbarius corneus Animals Azinphosmethyl Catalase Chlorpyrifos Enzyme Activation Fresh Water Gastropoda Glutathione Reductase Glutathione Transferase Insecticides Lipid Peroxidation Oxidative Stress Water Pollutants, Chemical |
| description |
Azinphos-methyl (AZM) and chlorpyrifos (CPF) are broad-spectrum organophosphate insecticides used for pest control on a number of food crops in many parts of the world that have been shown to inhibit cholinesterase activity in the non-target freshwater gastropod Planorbarius corneus. The present study was undertaken to determine: (a) whether AZM and CPF induce oxidative stress in P. corneus, and (b) whether a mixture of both organophosphates that causes a higher neurotoxicity than single pesticides also causes an enhanced oxidative stress. To this end, non-enzymatic and enzymatic parameters were measured in the soft tissues of snails acutely exposed to the insecticides in single-chemical (2.5mg AZML-1 and 7.5μgCPFL-1) and a binary-mixture (1.25mgAZML-1 plus 3.75μgCPFL-1) studies. At 24h, all pesticide-exposed groups showed significantly decreased glutathione (GSH) and glutathione disulfide (GSSG) levels when compared to control animals. At 48h, all exposed groups showed an alteration of the redox status (GSH/GSSG ratio) and a significant increase in malondialdehyde levels. The exposure for 48h to AZM and CPF, alone or in the binary mixture, also resulted in a significant decrease of the antioxidant superoxide dismutase activity. The greatest decrease was observed with CPF exposure (59% of decrease relative to the control group). A significant increase in catalase and glutathione S-transferase activities was observed in CPF group and in CPF and AZM+CPF groups, respectively. The activities of glutathione reductase and glucose 6-phosphate dehydrogenase did not show significant changes with respect to controls in any treatment group. In conclusion, the data shown in the present study provide evidence that AZM, CPF and a mixture of both organophosphates are able to induce oxidative stress and oxidative damage in P. corneus tissues. However, no similarities between the degree of neurotoxicity and the degree of alterations of the measured oxidative stress parameters were found. © 2015 Elsevier B.V. |
| format |
JOUR |
| author |
Cacciatore, L.C. Nemirovsky, S.I. Verrengia Guerrero, N.R. Cochón, A.C. |
| author_facet |
Cacciatore, L.C. Nemirovsky, S.I. Verrengia Guerrero, N.R. Cochón, A.C. |
| author_sort |
Cacciatore, L.C. |
| title |
Azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus |
| title_short |
Azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus |
| title_full |
Azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus |
| title_fullStr |
Azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus |
| title_full_unstemmed |
Azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus |
| title_sort |
azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod planorbarius corneus |
| url |
http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p12_Cacciatore |
| work_keys_str_mv |
AT cacciatorelc azinphosmethylandchlorpyrifosaloneorinabinarymixtureproduceoxidativestressandlipidperoxidationinthefreshwatergastropodplanorbariuscorneus AT nemirovskysi azinphosmethylandchlorpyrifosaloneorinabinarymixtureproduceoxidativestressandlipidperoxidationinthefreshwatergastropodplanorbariuscorneus AT verrengiaguerreronr azinphosmethylandchlorpyrifosaloneorinabinarymixtureproduceoxidativestressandlipidperoxidationinthefreshwatergastropodplanorbariuscorneus AT cochonac azinphosmethylandchlorpyrifosaloneorinabinarymixtureproduceoxidativestressandlipidperoxidationinthefreshwatergastropodplanorbariuscorneus |
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1807320586117971968 |
| spelling |
todo:paper_0166445X_v167_n_p12_Cacciatore2023-10-03T15:04:00Z Azinphos-methyl and chlorpyrifos, alone or in a binary mixture, produce oxidative stress and lipid peroxidation in the freshwater gastropod Planorbarius corneus Cacciatore, L.C. Nemirovsky, S.I. Verrengia Guerrero, N.R. Cochón, A.C. Antioxidant enzymes Invertebrates Mixtures Organophosphates Pesticides azinphos methyl catalase chlorpyrifos glucose 6 phosphate dehydrogenase glutathione glutathione disulfide glutathione reductase glutathione transferase malonaldehyde polyunsaturated fatty acid reactive oxygen metabolite superoxide dismutase azinphos methyl catalase chlorpyrifos fresh water glutathione reductase glutathione transferase insecticide water pollutant azinphos methyl chemical pollutant chlorpyrifos enzyme activity gastropod physiological response redox conditions toxicity adult animal experiment animal tissue Article comparative study concentration (parameters) controlled study environmental exposure enzyme activity freshwater species gastropod lipid peroxidation molecular dynamics neurotoxicity nonhuman oxidation reduction state oxidative stress Planorbarius corneus priority journal protein content protein expression protein localization tissue distribution upregulation animal drug effects enzyme activation gastropod lipid peroxidation metabolism oxidative stress toxicity water pollutant Animalia Gastropoda Invertebrata Planorbarius corneus Animals Azinphosmethyl Catalase Chlorpyrifos Enzyme Activation Fresh Water Gastropoda Glutathione Reductase Glutathione Transferase Insecticides Lipid Peroxidation Oxidative Stress Water Pollutants, Chemical Azinphos-methyl (AZM) and chlorpyrifos (CPF) are broad-spectrum organophosphate insecticides used for pest control on a number of food crops in many parts of the world that have been shown to inhibit cholinesterase activity in the non-target freshwater gastropod Planorbarius corneus. The present study was undertaken to determine: (a) whether AZM and CPF induce oxidative stress in P. corneus, and (b) whether a mixture of both organophosphates that causes a higher neurotoxicity than single pesticides also causes an enhanced oxidative stress. To this end, non-enzymatic and enzymatic parameters were measured in the soft tissues of snails acutely exposed to the insecticides in single-chemical (2.5mg AZML-1 and 7.5μgCPFL-1) and a binary-mixture (1.25mgAZML-1 plus 3.75μgCPFL-1) studies. At 24h, all pesticide-exposed groups showed significantly decreased glutathione (GSH) and glutathione disulfide (GSSG) levels when compared to control animals. At 48h, all exposed groups showed an alteration of the redox status (GSH/GSSG ratio) and a significant increase in malondialdehyde levels. The exposure for 48h to AZM and CPF, alone or in the binary mixture, also resulted in a significant decrease of the antioxidant superoxide dismutase activity. The greatest decrease was observed with CPF exposure (59% of decrease relative to the control group). A significant increase in catalase and glutathione S-transferase activities was observed in CPF group and in CPF and AZM+CPF groups, respectively. The activities of glutathione reductase and glucose 6-phosphate dehydrogenase did not show significant changes with respect to controls in any treatment group. In conclusion, the data shown in the present study provide evidence that AZM, CPF and a mixture of both organophosphates are able to induce oxidative stress and oxidative damage in P. corneus tissues. However, no similarities between the degree of neurotoxicity and the degree of alterations of the measured oxidative stress parameters were found. © 2015 Elsevier B.V. Fil:Nemirovsky, S.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Verrengia Guerrero, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cochón, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0166445X_v167_n_p12_Cacciatore |