Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)

Endosulfan (ES), a persistent organochlorine pesticide, is widely used despite its toxicity to non-target animals. Upon reaching water bodies, ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the action of ES on fish reproductive axis has been hard...

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Autores principales:	Da Cuña, R.H., Pandolfi, M., Genovese, G., Piazza, Y., Ansaldo, M., Lo Nostro, F.L.
Formato:	JOUR
Materias:	Cichlid fish Endocrine disruption Endosulfan Gonadotropins Sex steroids Testes pathology endosulfan follitropin beta subunit gamma glutamyltransferase gonadotropin luteinizing hormone endocrine disruptor morphology organochlorine perciform pesticide reproductive effort toxicity animal cell animal experiment animal tissue article brain function brain region cell function Cichlasoma dimerus cichlid controlled study ecotoxicology endocrine disease environmental exposure enzyme activity foam cell germ cell gonadotropin release histopathology hormone action hypophysis in vitro study in vivo study morphological trait nonhuman priority journal regulatory mechanism reproductive toxicity Sertoli cell spermatogenesis steroidogenesis testis function tissue culture Animals Cells, Cultured Cichlids Female Gonadotropins, Pituitary Male Ovary Pituitary Gland Testis Water Pollutants, Chemical South America Animalia Cichlasoma Cichlidae Perciformes
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0166445X_v126_n_p299_DaCuna
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0166445X_v126_n_p299_DaCuna
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spelling	todo:paper_0166445X_v126_n_p299_DaCuna2023-10-03T15:03:58Z Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae) Da Cuña, R.H. Pandolfi, M. Genovese, G. Piazza, Y. Ansaldo, M. Lo Nostro, F.L. Cichlid fish Endocrine disruption Endosulfan Gonadotropins Sex steroids Testes pathology endosulfan follitropin beta subunit gamma glutamyltransferase gonadotropin luteinizing hormone endocrine disruptor endosulfan morphology organochlorine perciform pesticide reproductive effort toxicity animal cell animal experiment animal tissue article brain function brain region cell function Cichlasoma dimerus cichlid controlled study ecotoxicology endocrine disease environmental exposure enzyme activity foam cell germ cell gonadotropin release histopathology hormone action hypophysis in vitro study in vivo study morphological trait nonhuman priority journal regulatory mechanism reproductive toxicity Sertoli cell spermatogenesis steroidogenesis testis function tissue culture Animals Cells, Cultured Cichlids Endosulfan Female Gonadotropins, Pituitary Male Ovary Pituitary Gland Testis Water Pollutants, Chemical South America Animalia Cichlasoma Cichlidae Perciformes Endosulfan (ES), a persistent organochlorine pesticide, is widely used despite its toxicity to non-target animals. Upon reaching water bodies, ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the action of ES on fish reproductive axis has been hardly studied thus far. The aim of the present work was to assess the endocrine disruptive potential of endosulfan on the pituitary gonadotropins levels and on the testes function due to ES in the South American freshwater fish Cichlasoma dimerus, using in vitro and in vivo approaches. In vitro experiments showed that ES inhibited the LH-stimulated steroidogenesis in gonads; no change was observed in gonadotropins release from pituitaries in culture. Laboratory waterborne ES (0.1, 0.3 and 1 μg/L) exposure for two months caused decrease in βFSH pituitary content and γGT activity in the testes (Sertoli cell function marker). Testicular histology revealed pathologies such as scarce intermediate stages of spermatogenesis, release of immature germ cells into the lobular lumen, presence of foam cells and interstitial fibrosis. As FSH and FSH-mediated steroidogenesis regulate spermatogenesis and Sertoli cell function, the effect of ES on FSH could be responsible for the morphological alterations observed in testes. In vitro, ES disrupted steroidogenesis in gonads, therefore similar effects in vivo cannot be ruled out. Based on this evidence, ES exhibits an endocrine disruptive action on the reproductive axis of C. dimerus, causing disruption at the pituitary and/or at the gonad level. These effects could acquire ecological significance under prolonged exposure to the pesticide in nature. © 2012 Elsevier B.V. Fil:Da Cuña, R.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piazza, Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ansaldo, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0166445X_v126_n_p299_DaCuna
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cichlid fish Endocrine disruption Endosulfan Gonadotropins Sex steroids Testes pathology endosulfan follitropin beta subunit gamma glutamyltransferase gonadotropin luteinizing hormone endocrine disruptor endosulfan morphology organochlorine perciform pesticide reproductive effort toxicity animal cell animal experiment animal tissue article brain function brain region cell function Cichlasoma dimerus cichlid controlled study ecotoxicology endocrine disease environmental exposure enzyme activity foam cell germ cell gonadotropin release histopathology hormone action hypophysis in vitro study in vivo study morphological trait nonhuman priority journal regulatory mechanism reproductive toxicity Sertoli cell spermatogenesis steroidogenesis testis function tissue culture Animals Cells, Cultured Cichlids Endosulfan Female Gonadotropins, Pituitary Male Ovary Pituitary Gland Testis Water Pollutants, Chemical South America Animalia Cichlasoma Cichlidae Perciformes
spellingShingle	Cichlid fish Endocrine disruption Endosulfan Gonadotropins Sex steroids Testes pathology endosulfan follitropin beta subunit gamma glutamyltransferase gonadotropin luteinizing hormone endocrine disruptor endosulfan morphology organochlorine perciform pesticide reproductive effort toxicity animal cell animal experiment animal tissue article brain function brain region cell function Cichlasoma dimerus cichlid controlled study ecotoxicology endocrine disease environmental exposure enzyme activity foam cell germ cell gonadotropin release histopathology hormone action hypophysis in vitro study in vivo study morphological trait nonhuman priority journal regulatory mechanism reproductive toxicity Sertoli cell spermatogenesis steroidogenesis testis function tissue culture Animals Cells, Cultured Cichlids Endosulfan Female Gonadotropins, Pituitary Male Ovary Pituitary Gland Testis Water Pollutants, Chemical South America Animalia Cichlasoma Cichlidae Perciformes Da Cuña, R.H. Pandolfi, M. Genovese, G. Piazza, Y. Ansaldo, M. Lo Nostro, F.L. Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)
topic_facet	Cichlid fish Endocrine disruption Endosulfan Gonadotropins Sex steroids Testes pathology endosulfan follitropin beta subunit gamma glutamyltransferase gonadotropin luteinizing hormone endocrine disruptor endosulfan morphology organochlorine perciform pesticide reproductive effort toxicity animal cell animal experiment animal tissue article brain function brain region cell function Cichlasoma dimerus cichlid controlled study ecotoxicology endocrine disease environmental exposure enzyme activity foam cell germ cell gonadotropin release histopathology hormone action hypophysis in vitro study in vivo study morphological trait nonhuman priority journal regulatory mechanism reproductive toxicity Sertoli cell spermatogenesis steroidogenesis testis function tissue culture Animals Cells, Cultured Cichlids Endosulfan Female Gonadotropins, Pituitary Male Ovary Pituitary Gland Testis Water Pollutants, Chemical South America Animalia Cichlasoma Cichlidae Perciformes
description	Endosulfan (ES), a persistent organochlorine pesticide, is widely used despite its toxicity to non-target animals. Upon reaching water bodies, ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the action of ES on fish reproductive axis has been hardly studied thus far. The aim of the present work was to assess the endocrine disruptive potential of endosulfan on the pituitary gonadotropins levels and on the testes function due to ES in the South American freshwater fish Cichlasoma dimerus, using in vitro and in vivo approaches. In vitro experiments showed that ES inhibited the LH-stimulated steroidogenesis in gonads; no change was observed in gonadotropins release from pituitaries in culture. Laboratory waterborne ES (0.1, 0.3 and 1 μg/L) exposure for two months caused decrease in βFSH pituitary content and γGT activity in the testes (Sertoli cell function marker). Testicular histology revealed pathologies such as scarce intermediate stages of spermatogenesis, release of immature germ cells into the lobular lumen, presence of foam cells and interstitial fibrosis. As FSH and FSH-mediated steroidogenesis regulate spermatogenesis and Sertoli cell function, the effect of ES on FSH could be responsible for the morphological alterations observed in testes. In vitro, ES disrupted steroidogenesis in gonads, therefore similar effects in vivo cannot be ruled out. Based on this evidence, ES exhibits an endocrine disruptive action on the reproductive axis of C. dimerus, causing disruption at the pituitary and/or at the gonad level. These effects could acquire ecological significance under prolonged exposure to the pesticide in nature. © 2012 Elsevier B.V.
format	JOUR
author	Da Cuña, R.H. Pandolfi, M. Genovese, G. Piazza, Y. Ansaldo, M. Lo Nostro, F.L.
author_facet	Da Cuña, R.H. Pandolfi, M. Genovese, G. Piazza, Y. Ansaldo, M. Lo Nostro, F.L.
author_sort	Da Cuña, R.H.
title	Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)
title_short	Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)
title_full	Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)
title_fullStr	Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)
title_full_unstemmed	Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)
title_sort	endocrine disruptive potential of endosulfan on the reproductive axis of cichlasoma dimerus (perciformes, cichlidae)
url	http://hdl.handle.net/20.500.12110/paper_0166445X_v126_n_p299_DaCuna
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Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)

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