Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L
A partially purified plant inhibitor (Meliacin) isolated from Melia azedarach L induced in cells a refractory state to virus infection. Meliacin was active in a large variety of continuous and/or primary cell cultures. A state of maximum virus resistance was achieved after 2 h of incubation and was...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01663542_v9_n4_p221_Andrei |
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todo:paper_01663542_v9_n4_p221_Andrei2023-10-03T15:03:54Z Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L Andrei, G.M. Damonte, E.B. de Torres, R.A. Coto, C.E. Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication A partially purified plant inhibitor (Meliacin) isolated from Melia azedarach L induced in cells a refractory state to virus infection. Meliacin was active in a large variety of continuous and/or primary cell cultures. A state of maximum virus resistance was achieved after 2 h of incubation and was maintained for at least 15 h; later on it declined but it was fully regained after a second pulse of Meliacin. Interferon was not detected in the supernatant of cells treated with Meliacin and a measurable increase in ds-RNA dependent protein kinase activity was not observed in extracts of Meliacin-treated cells. The antiviral state was not transferred by either extracellular fluid or direct cell-to-cell contact. An active cell metabolism was required for Meliacin action, which was partially reversed in the presence of actinomycin D. It appears that Meliacin is not an interferon-like substance, which induces an antiviral state based on a still unexplained mechanism. © 1988. Fil:Andrei, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01663542_v9_n4_p221_Andrei |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication |
| spellingShingle |
Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication Andrei, G.M. Damonte, E.B. de Torres, R.A. Coto, C.E. Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L |
| topic_facet |
Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication |
| description |
A partially purified plant inhibitor (Meliacin) isolated from Melia azedarach L induced in cells a refractory state to virus infection. Meliacin was active in a large variety of continuous and/or primary cell cultures. A state of maximum virus resistance was achieved after 2 h of incubation and was maintained for at least 15 h; later on it declined but it was fully regained after a second pulse of Meliacin. Interferon was not detected in the supernatant of cells treated with Meliacin and a measurable increase in ds-RNA dependent protein kinase activity was not observed in extracts of Meliacin-treated cells. The antiviral state was not transferred by either extracellular fluid or direct cell-to-cell contact. An active cell metabolism was required for Meliacin action, which was partially reversed in the presence of actinomycin D. It appears that Meliacin is not an interferon-like substance, which induces an antiviral state based on a still unexplained mechanism. © 1988. |
| format |
JOUR |
| author |
Andrei, G.M. Damonte, E.B. de Torres, R.A. Coto, C.E. |
| author_facet |
Andrei, G.M. Damonte, E.B. de Torres, R.A. Coto, C.E. |
| author_sort |
Andrei, G.M. |
| title |
Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L |
| title_short |
Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L |
| title_full |
Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L |
| title_fullStr |
Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L |
| title_full_unstemmed |
Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L |
| title_sort |
induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of melia azedarach l |
| url |
http://hdl.handle.net/20.500.12110/paper_01663542_v9_n4_p221_Andrei |
| work_keys_str_mv |
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| _version_ |
1807318465401323520 |