Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays

A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody...

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Detalles Bibliográficos
Autores principales: Pujol, C.A., Feledi, C.A., Massouh, E.J.
Formato: JOUR
Lenguaje:English
Materias:
virus antibody
virus antigen
cell culture
foot and mouth disease virus
hemagglutination
nonhuman
priority journal
serum
Animal
Antigens, Viral
Aphthovirus
Erythrocytes
Hemagglutination Inhibition Tests
Hemagglutination Tests
Hemolytic Plaque Technique
Immunologic Techniques
In Vitro
Microbiological Techniques
Sheep
Support, Non-U.S. Gov't
Tannic Acid
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01660934_v20_n4_p275_Pujol
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglutination (PH), passive hemagglutination inhibition (PHI) and passive immune hemolysis (PIL) assays. Fresh and SRBC stored in Alsever's solution showed similar behavior when used as indicator cells. Optimal sensitization of erythrocytes was achieved using tannic acid 1:20000 and 20 μg of purified virus/ml at pH 7.6. Specificity of the reaction was confirmed by PH and PHI in homologous and heterologous systems. The coupled antigen-antibody complex was sensitive to complement mediated lysis in a PIL test.

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