Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays
A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody...
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01660934_v20_n4_p275_Pujol |
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todo:paper_01660934_v20_n4_p275_Pujol2023-10-03T15:03:09Z Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays Pujol, C.A. Feledi, C.A. Massouh, E.J. virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglutination (PH), passive hemagglutination inhibition (PHI) and passive immune hemolysis (PIL) assays. Fresh and SRBC stored in Alsever's solution showed similar behavior when used as indicator cells. Optimal sensitization of erythrocytes was achieved using tannic acid 1:20000 and 20 μg of purified virus/ml at pH 7.6. Specificity of the reaction was confirmed by PH and PHI in homologous and heterologous systems. The coupled antigen-antibody complex was sensitive to complement mediated lysis in a PIL test. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Massouh, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01660934_v20_n4_p275_Pujol |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
language |
English |
orig_language_str_mv |
English |
topic |
virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid |
spellingShingle |
virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid Pujol, C.A. Feledi, C.A. Massouh, E.J. Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays |
topic_facet |
virus antibody virus antigen cell culture foot and mouth disease virus hemagglutination nonhuman priority journal serum Animal Antigens, Viral Aphthovirus Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hemolytic Plaque Technique Immunologic Techniques In Vitro Microbiological Techniques Sheep Support, Non-U.S. Gov't Tannic Acid |
description |
A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglutination (PH), passive hemagglutination inhibition (PHI) and passive immune hemolysis (PIL) assays. Fresh and SRBC stored in Alsever's solution showed similar behavior when used as indicator cells. Optimal sensitization of erythrocytes was achieved using tannic acid 1:20000 and 20 μg of purified virus/ml at pH 7.6. Specificity of the reaction was confirmed by PH and PHI in homologous and heterologous systems. The coupled antigen-antibody complex was sensitive to complement mediated lysis in a PIL test. |
format |
JOUR |
author |
Pujol, C.A. Feledi, C.A. Massouh, E.J. |
author_facet |
Pujol, C.A. Feledi, C.A. Massouh, E.J. |
author_sort |
Pujol, C.A. |
title |
Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays |
title_short |
Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays |
title_full |
Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays |
title_fullStr |
Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays |
title_full_unstemmed |
Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays |
title_sort |
coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays |
url |
http://hdl.handle.net/20.500.12110/paper_01660934_v20_n4_p275_Pujol |
work_keys_str_mv |
AT pujolca couplingoffootandmouthdiseasevirustosheepredbloodcellsusingtannicacidforimmunologicalassays AT feledica couplingoffootandmouthdiseasevirustosheepredbloodcellsusingtannicacidforimmunologicalassays AT massouhej couplingoffootandmouthdiseasevirustosheepredbloodcellsusingtannicacidforimmunologicalassays |
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