Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice

We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and in...

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Autores principales:	Refojo, D., Kovalovsky, D., Young, J.I., Rubinstein, M., Holsboer, F., Reul, J.M.H.M, Low, M.J., Arzt, E.
Formato:	JOUR
Materias:	β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01655728_v131_n1-2_p126_Refojo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01655728_v131_n1-2_p126_Refojo
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spelling	todo:paper_01655728_v131_n1-2_p126_Refojo2023-10-03T15:02:57Z Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice Refojo, D. Kovalovsky, D. Young, J.I. Rubinstein, M. Holsboer, F. Reul, J.M.H.M Low, M.J. Arzt, E. β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Cytokines Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and interleukin (IL)-2 mRNA levels, in vitro production of the splenic macrophage inflammatory cytokines IL-6 and Tumor Necrosis Factor (TNF)-α and plasma IL-6 following lipopolysaccharide (LPS) administration. β-Endorphin-deficient mice had attenuated increases of plasma ACTH and corticosterone levels in response to LPS. These results are consistent with a postulated inhibitory role of endogenous β-endorphin on the immune system at multiple levels. © 2002 Elsevier Science B.V. All rights reserved. Fil:Refojo, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kovalovsky, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Young, J.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rubinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01655728_v131_n1-2_p126_Refojo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Cytokines Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha
spellingShingle	β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Cytokines Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha Refojo, D. Kovalovsky, D. Young, J.I. Rubinstein, M. Holsboer, F. Reul, J.M.H.M Low, M.J. Arzt, E. Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
topic_facet	β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Cytokines Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha
description	We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and interleukin (IL)-2 mRNA levels, in vitro production of the splenic macrophage inflammatory cytokines IL-6 and Tumor Necrosis Factor (TNF)-α and plasma IL-6 following lipopolysaccharide (LPS) administration. β-Endorphin-deficient mice had attenuated increases of plasma ACTH and corticosterone levels in response to LPS. These results are consistent with a postulated inhibitory role of endogenous β-endorphin on the immune system at multiple levels. © 2002 Elsevier Science B.V. All rights reserved.
format	JOUR
author	Refojo, D. Kovalovsky, D. Young, J.I. Rubinstein, M. Holsboer, F. Reul, J.M.H.M Low, M.J. Arzt, E.
author_facet	Refojo, D. Kovalovsky, D. Young, J.I. Rubinstein, M. Holsboer, F. Reul, J.M.H.M Low, M.J. Arzt, E.
author_sort	Refojo, D.
title	Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_short	Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_full	Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_fullStr	Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_full_unstemmed	Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_sort	increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
url	http://hdl.handle.net/20.500.12110/paper_01655728_v131_n1-2_p126_Refojo
work_keys_str_mv	AT refojod increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT kovalovskyd increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT youngji increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT rubinsteinm increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT holsboerf increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT reuljmhm increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT lowmj increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT arzte increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice
_version_	1807315869851713536

Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice

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