Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma

The seminal plasma and a fraction of the spermatozoal membrane solubilised by sarkosyl contain similar antigenic determinants. The glycoprotein isolated from the seminal plasma also contains determinants which are immunologically identical to those of the sarkosyl soluble fraction. Three of these de...

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Autores principales: de Cerezo, J.M.S., Bueno, M.P., Skowroñski, B., Cerezo, A.S.
Formato: JOUR
Materias:
glycoprotein
sperm antibody
immunohistochemistry
male genital system
normal human
sperm
spermatozoon
Antigens
Epitopes
Glycoproteins
Hemagglutination Tests
Human
Immune Sera
Immunodiffusion
Lectins
Male
Receptors, Immunologic
Semen
Spermatozoa
Streptococcus pneumoniae
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01650378_v4_n1_p11_deCerezo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01650378_v4_n1_p11_deCerezo
record_format dspace
spelling todo:paper_01650378_v4_n1_p11_deCerezo2023-10-03T15:02:39Z Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma de Cerezo, J.M.S. Bueno, M.P. Skowroñski, B. Cerezo, A.S. glycoprotein sperm antibody immunohistochemistry male genital system normal human sperm spermatozoon Antigens Epitopes Glycoproteins Hemagglutination Tests Human Immune Sera Immunodiffusion Lectins Male Receptors, Immunologic Semen Spermatozoa Streptococcus pneumoniae Support, Non-U.S. Gov't The seminal plasma and a fraction of the spermatozoal membrane solubilised by sarkosyl contain similar antigenic determinants. The glycoprotein isolated from the seminal plasma also contains determinants which are immunologically identical to those of the sarkosyl soluble fraction. Three of these determinants were identified as: two oligosaccharide chains with terminal N-acetyl neuraminic acid and with subterminal α-l-fucopyranose and β-d-galactopyranose residues respectively, and an oligosaccharide chain with β-d-galactopyranose as non-reducing end-chain. The receptors on the spermatozoa to anti-glycoprotein and anti seminal plasma antibodies are localised. The specificity of the receptors localised in the neck region depends entirely on the presence of N-acetyl neuraminic acid as end-chain group while the specificity of the receptors to the anti-seminal plasma antibodies in the intermediate segment plus tail and to the anti-glycoprotein antibodies in the post-nuclear cap depends also on the subterminal units. At least two other receptors in the equatorial zone are ended by residues different from the N-acetyl neuraminic acid. One of them, which is also found in the acrosome, does not have specificity for anti-glycoprotein antibodies, while the other, which is not in the acrosome, has anti-glycoprotein antibodies specificity and may have a terminal β-d-galactopyranose residue. The glycoprotein antigen produces sperm-agglutinating, sperm-immobilizing and cytotoxic antibodies in rabbits. © 1982. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01650378_v4_n1_p11_deCerezo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic glycoprotein
sperm antibody
immunohistochemistry
male genital system
normal human
sperm
spermatozoon
Antigens
Epitopes
Glycoproteins
Hemagglutination Tests
Human
Immune Sera
Immunodiffusion
Lectins
Male
Receptors, Immunologic
Semen
Spermatozoa
Streptococcus pneumoniae
Support, Non-U.S. Gov't
spellingShingle glycoprotein
sperm antibody
immunohistochemistry
male genital system
normal human
sperm
spermatozoon
Antigens
Epitopes
Glycoproteins
Hemagglutination Tests
Human
Immune Sera
Immunodiffusion
Lectins
Male
Receptors, Immunologic
Semen
Spermatozoa
Streptococcus pneumoniae
Support, Non-U.S. Gov't
de Cerezo, J.M.S.
Bueno, M.P.
Skowroñski, B.
Cerezo, A.S.
Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma
topic_facet glycoprotein
sperm antibody
immunohistochemistry
male genital system
normal human
sperm
spermatozoon
Antigens
Epitopes
Glycoproteins
Hemagglutination Tests
Human
Immune Sera
Immunodiffusion
Lectins
Male
Receptors, Immunologic
Semen
Spermatozoa
Streptococcus pneumoniae
Support, Non-U.S. Gov't
description The seminal plasma and a fraction of the spermatozoal membrane solubilised by sarkosyl contain similar antigenic determinants. The glycoprotein isolated from the seminal plasma also contains determinants which are immunologically identical to those of the sarkosyl soluble fraction. Three of these determinants were identified as: two oligosaccharide chains with terminal N-acetyl neuraminic acid and with subterminal α-l-fucopyranose and β-d-galactopyranose residues respectively, and an oligosaccharide chain with β-d-galactopyranose as non-reducing end-chain. The receptors on the spermatozoa to anti-glycoprotein and anti seminal plasma antibodies are localised. The specificity of the receptors localised in the neck region depends entirely on the presence of N-acetyl neuraminic acid as end-chain group while the specificity of the receptors to the anti-seminal plasma antibodies in the intermediate segment plus tail and to the anti-glycoprotein antibodies in the post-nuclear cap depends also on the subterminal units. At least two other receptors in the equatorial zone are ended by residues different from the N-acetyl neuraminic acid. One of them, which is also found in the acrosome, does not have specificity for anti-glycoprotein antibodies, while the other, which is not in the acrosome, has anti-glycoprotein antibodies specificity and may have a terminal β-d-galactopyranose residue. The glycoprotein antigen produces sperm-agglutinating, sperm-immobilizing and cytotoxic antibodies in rabbits. © 1982.
format JOUR
author de Cerezo, J.M.S.
Bueno, M.P.
Skowroñski, B.
Cerezo, A.S.
author_facet de Cerezo, J.M.S.
Bueno, M.P.
Skowroñski, B.
Cerezo, A.S.
author_sort de Cerezo, J.M.S.
title Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma
title_short Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma
title_full Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma
title_fullStr Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma
title_full_unstemmed Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma
title_sort immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma
url http://hdl.handle.net/20.500.12110/paper_01650378_v4_n1_p11_deCerezo
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AT buenomp immunoserologicalimmunohistochemicalandimmunobiologicalstudiesonanantigenicglycoproteinisolatedfromhumanseminalplasma
AT skowronskib immunoserologicalimmunohistochemicalandimmunobiologicalstudiesonanantigenicglycoproteinisolatedfromhumanseminalplasma
AT cerezoas immunoserologicalimmunohistochemicalandimmunobiologicalstudiesonanantigenicglycoproteinisolatedfromhumanseminalplasma
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