Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polygl...
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todo:paper_01476513_v74_n4_p741_Romero2023-10-03T15:00:46Z Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri Romero, D.M. Ríos de Molina, M.C. Juárez, Á.B. Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical glyphosate green alga inhibition microalga oxidation pollution exposure surfactant algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella Chlorella kessleri chlorophyll content controlled study cytotoxicity test enzyme activity growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Chlorella Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc. Fil:Romero, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ríos de Molina, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Juárez, Á.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v74_n4_p741_Romero |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical glyphosate green alga inhibition microalga oxidation pollution exposure surfactant algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella Chlorella kessleri chlorophyll content controlled study cytotoxicity test enzyme activity growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Chlorella Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri |
spellingShingle |
Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical glyphosate green alga inhibition microalga oxidation pollution exposure surfactant algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella Chlorella kessleri chlorophyll content controlled study cytotoxicity test enzyme activity growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Chlorella Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri Romero, D.M. Ríos de Molina, M.C. Juárez, Á.B. Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
topic_facet |
Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical glyphosate green alga inhibition microalga oxidation pollution exposure surfactant algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella Chlorella kessleri chlorophyll content controlled study cytotoxicity test enzyme activity growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Chlorella Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri |
description |
We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc. |
format |
JOUR |
author |
Romero, D.M. Ríos de Molina, M.C. Juárez, Á.B. |
author_facet |
Romero, D.M. Ríos de Molina, M.C. Juárez, Á.B. |
author_sort |
Romero, D.M. |
title |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_short |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_full |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_fullStr |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_full_unstemmed |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_sort |
oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of chlorella kessleri |
url |
http://hdl.handle.net/20.500.12110/paper_01476513_v74_n4_p741_Romero |
work_keys_str_mv |
AT romerodm oxidativestressinducedbyacommercialglyphosateformulationinatolerantstrainofchlorellakessleri AT riosdemolinamc oxidativestressinducedbyacommercialglyphosateformulationinatolerantstrainofchlorellakessleri AT juarezab oxidativestressinducedbyacommercialglyphosateformulationinatolerantstrainofchlorellakessleri |
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1782024777896034304 |