ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus

ALA-D activity and lead concentrations were measured in blood and liver tissues of the fish Prochilodus lineatus, collected from three locations along the coast of the La Plata River, Argentina. Two of them, Berazategui and Berisso, were located nearby the main ducts that discharge the urban and dom...

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Autores principales:	Lombardi, P.E., Peri, S.I., Verrengia Guerrero, N.R.
Formato:	JOUR
Materias:	ALA-D activity La Plata River Lead Prochilodus lineatus Reactivation index Sewage discharges lead porphobilinogen synthase bioaccumulation biomarker biomonitoring blood cell organelle concentration (composition) dose-response relationship ecotoxicology enzyme activity fish inhibition pollution exposure reactivation animal tissue Argentina article contaminated fish controlled study domestic waste environmental exposure enzyme inhibition enzyme reactivation female lead blood level lead liver level liver level male nonhuman river sewage water contamination water sampling Animals Biological Markers Environmental Monitoring Environmental Pollutants Enzyme Reactivators Fishes Liver Porphobilinogen Synthase Rivers Zinc La Plata Basin Atalaya
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01476513_v73_n7_p1704_Lombardi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01476513_v73_n7_p1704_Lombardi2023-10-03T15:00:45Z ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus Lombardi, P.E. Peri, S.I. Verrengia Guerrero, N.R. ALA-D activity La Plata River Lead Prochilodus lineatus Reactivation index Sewage discharges lead porphobilinogen synthase bioaccumulation biomarker biomonitoring blood cell organelle concentration (composition) dose-response relationship ecotoxicology enzyme activity fish inhibition lead pollution exposure reactivation animal tissue Argentina article bioaccumulation contaminated fish controlled study domestic waste environmental exposure enzyme activity enzyme inhibition enzyme reactivation female lead blood level lead liver level liver level male nonhuman Prochilodus lineatus river sewage water contamination water sampling Animals Argentina Biological Markers Environmental Monitoring Environmental Pollutants Enzyme Reactivators Fishes Lead Liver Porphobilinogen Synthase Rivers Zinc Argentina La Plata Basin Atalaya Prochilodus lineatus ALA-D activity and lead concentrations were measured in blood and liver tissues of the fish Prochilodus lineatus, collected from three locations along the coast of the La Plata River, Argentina. Two of them, Berazategui and Berisso, were located nearby the main ducts that discharge the urban and domestic waste disposal from Buenos Aires and La Plata cities, respectively, while the third station (Atalaya) was free of sewage discharges. For both tissues, the levels of lead in fish from Berazategui and Berisso were higher than those found in the samples from Atalaya. For blood, but not for liver, a significant negative correlation was found between ALA-activity and tissue levels of lead considering all the data. However, no good correlations were observed at each location. Therefore, an enzyme reactivation technique was optimized. The blood enzyme, but not the liver one, could be effectively reactivated with zinc (ZnII). The values of the reactivated ALA-D in samples from Berazategui and Berisso, but not from Atalaya, were significantly higher than the original values, indicating that the enzyme was actually inhibited. In addition, the reactivation index showed significant correlations with the blood lead levels. It is proposed that the reactivation index, rather than the ALA-D activity, may reflect better the extent of lead contamination, especially for field monitoring programs where many confounding factors may affect the biomarker response. © 2010 Elsevier Inc. Fil:Verrengia Guerrero, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v73_n7_p1704_Lombardi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ALA-D activity La Plata River Lead Prochilodus lineatus Reactivation index Sewage discharges lead porphobilinogen synthase bioaccumulation biomarker biomonitoring blood cell organelle concentration (composition) dose-response relationship ecotoxicology enzyme activity fish inhibition lead pollution exposure reactivation animal tissue Argentina article bioaccumulation contaminated fish controlled study domestic waste environmental exposure enzyme activity enzyme inhibition enzyme reactivation female lead blood level lead liver level liver level male nonhuman Prochilodus lineatus river sewage water contamination water sampling Animals Argentina Biological Markers Environmental Monitoring Environmental Pollutants Enzyme Reactivators Fishes Lead Liver Porphobilinogen Synthase Rivers Zinc Argentina La Plata Basin Atalaya Prochilodus lineatus
spellingShingle	ALA-D activity La Plata River Lead Prochilodus lineatus Reactivation index Sewage discharges lead porphobilinogen synthase bioaccumulation biomarker biomonitoring blood cell organelle concentration (composition) dose-response relationship ecotoxicology enzyme activity fish inhibition lead pollution exposure reactivation animal tissue Argentina article bioaccumulation contaminated fish controlled study domestic waste environmental exposure enzyme activity enzyme inhibition enzyme reactivation female lead blood level lead liver level liver level male nonhuman Prochilodus lineatus river sewage water contamination water sampling Animals Argentina Biological Markers Environmental Monitoring Environmental Pollutants Enzyme Reactivators Fishes Lead Liver Porphobilinogen Synthase Rivers Zinc Argentina La Plata Basin Atalaya Prochilodus lineatus Lombardi, P.E. Peri, S.I. Verrengia Guerrero, N.R. ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus
topic_facet	ALA-D activity La Plata River Lead Prochilodus lineatus Reactivation index Sewage discharges lead porphobilinogen synthase bioaccumulation biomarker biomonitoring blood cell organelle concentration (composition) dose-response relationship ecotoxicology enzyme activity fish inhibition lead pollution exposure reactivation animal tissue Argentina article bioaccumulation contaminated fish controlled study domestic waste environmental exposure enzyme activity enzyme inhibition enzyme reactivation female lead blood level lead liver level liver level male nonhuman Prochilodus lineatus river sewage water contamination water sampling Animals Argentina Biological Markers Environmental Monitoring Environmental Pollutants Enzyme Reactivators Fishes Lead Liver Porphobilinogen Synthase Rivers Zinc Argentina La Plata Basin Atalaya Prochilodus lineatus
description	ALA-D activity and lead concentrations were measured in blood and liver tissues of the fish Prochilodus lineatus, collected from three locations along the coast of the La Plata River, Argentina. Two of them, Berazategui and Berisso, were located nearby the main ducts that discharge the urban and domestic waste disposal from Buenos Aires and La Plata cities, respectively, while the third station (Atalaya) was free of sewage discharges. For both tissues, the levels of lead in fish from Berazategui and Berisso were higher than those found in the samples from Atalaya. For blood, but not for liver, a significant negative correlation was found between ALA-activity and tissue levels of lead considering all the data. However, no good correlations were observed at each location. Therefore, an enzyme reactivation technique was optimized. The blood enzyme, but not the liver one, could be effectively reactivated with zinc (ZnII). The values of the reactivated ALA-D in samples from Berazategui and Berisso, but not from Atalaya, were significantly higher than the original values, indicating that the enzyme was actually inhibited. In addition, the reactivation index showed significant correlations with the blood lead levels. It is proposed that the reactivation index, rather than the ALA-D activity, may reflect better the extent of lead contamination, especially for field monitoring programs where many confounding factors may affect the biomarker response. © 2010 Elsevier Inc.
format	JOUR
author	Lombardi, P.E. Peri, S.I. Verrengia Guerrero, N.R.
author_facet	Lombardi, P.E. Peri, S.I. Verrengia Guerrero, N.R.
author_sort	Lombardi, P.E.
title	ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus
title_short	ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus
title_full	ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus
title_fullStr	ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus
title_full_unstemmed	ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus
title_sort	ala-d and ala-d reactivated as biomarkers of lead contamination in the fish prochilodus lineatus
url	http://hdl.handle.net/20.500.12110/paper_01476513_v73_n7_p1704_Lombardi
work_keys_str_mv	AT lombardipe aladandaladreactivatedasbiomarkersofleadcontaminationinthefishprochiloduslineatus AT perisi aladandaladreactivatedasbiomarkersofleadcontaminationinthefishprochiloduslineatus AT verrengiaguerreronr aladandaladreactivatedasbiomarkersofleadcontaminationinthefishprochiloduslineatus
_version_	1807314592592822272

ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus

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