Environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod Biomphalaria straminea
Organophosphate insecticides (OPs) are commonly used in Argentina and around the world for pest control in food crops. They exert their toxicity through the inhibition of the enzyme acetylcholinesterase. In the present study, we aimed to evaluate biochemical and reproductive effects in Biomphalaria...
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todo:paper_01476513_v162_n_p287_Cossi2023-10-03T15:00:41Z Environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod Biomphalaria straminea Cossi, P.F. Herbert, L.T. Yusseppone, M.S. Pérez, A.F. Kristoff, G. Antioxidants B-esterases Biomarkers Insecticide Invertebrates Reproduction azinphos methyl biological marker carboxylesterase catalase cholinesterase glutathione transferase glycogen protein reactive oxygen metabolite superoxide dismutase azinphos methyl carboxylesterase catalase cholinesterase fresh water glutathione transferase insecticide reactive oxygen metabolite superoxide dismutase antioxidant biomarker concentration (composition) gastropod insecticide invertebrate pollution exposure reproductive behavior toxicity adult antioxidant activity Argentina Article biochemical analysis Biomphalaria straminea chemical parameters concentration (parameters) controlled study environmental exposure enzyme activity hatching nonhuman protein analysis reproduction survival rate toxicity testing urogenital system parameters zygote animal Biomphalaria drug effect metabolism physiology toxicity water pollutant Argentina Biomphalaria straminea Gastropoda Invertebrata Animals Azinphosmethyl Biomarkers Biomphalaria Carboxylic Ester Hydrolases Catalase Cholinesterases Fresh Water Glutathione Transferase Insecticides Reactive Oxygen Species Reproduction Superoxide Dismutase Water Pollutants, Chemical Organophosphate insecticides (OPs) are commonly used in Argentina and around the world for pest control in food crops. They exert their toxicity through the inhibition of the enzyme acetylcholinesterase. In the present study, we aimed to evaluate biochemical and reproductive effects in Biomphalaria straminea, a freshwater gastropod naturally distributed in Argentina, of subchronic exposures to environmental azinphos-methyl concentrations (20 and 200 µg L−1). For biochemical parameters, adult organisms were exposed for 14 days and the activity of cholinesterases (ChEs), carboxylesterases (CEs), glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), the production of reactive oxygen species (ROS), the total antioxidant capacity (TAC), glycogen and proteins were determined. For reproductive parameters, the egg masses of B. straminea were exposed to azinphos-methyl for one month, and the hatching time and success as well as the offspring survival were registered. We found different toxic effects elicited by the insecticide on the studied biomarkers. CEs activity was significantly inhibited while CAT and GST activities, ROS production and TAC were significantly increased, with respect to the solvent control group. ChE and SOD activities and protein and glycogen contents were not altered by azinphos-methyl. The hatching time and success were not statistically different from control. Nevertheless, the offspring survival was severely affected by the insecticide. Our results show that the primary target of the insecticide (ChE) was not inhibited but CEs, GST, CAT, ROS, TAC and offspring survival were sensitive biomarkers and valuable endpoints for subchronic toxicity assessments in this species. © 2018 Elsevier Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v162_n_p287_Cossi |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antioxidants B-esterases Biomarkers Insecticide Invertebrates Reproduction azinphos methyl biological marker carboxylesterase catalase cholinesterase glutathione transferase glycogen protein reactive oxygen metabolite superoxide dismutase azinphos methyl carboxylesterase catalase cholinesterase fresh water glutathione transferase insecticide reactive oxygen metabolite superoxide dismutase antioxidant biomarker concentration (composition) gastropod insecticide invertebrate pollution exposure reproductive behavior toxicity adult antioxidant activity Argentina Article biochemical analysis Biomphalaria straminea chemical parameters concentration (parameters) controlled study environmental exposure enzyme activity hatching nonhuman protein analysis reproduction survival rate toxicity testing urogenital system parameters zygote animal Biomphalaria drug effect metabolism physiology toxicity water pollutant Argentina Biomphalaria straminea Gastropoda Invertebrata Animals Azinphosmethyl Biomarkers Biomphalaria Carboxylic Ester Hydrolases Catalase Cholinesterases Fresh Water Glutathione Transferase Insecticides Reactive Oxygen Species Reproduction Superoxide Dismutase Water Pollutants, Chemical |
| spellingShingle |
Antioxidants B-esterases Biomarkers Insecticide Invertebrates Reproduction azinphos methyl biological marker carboxylesterase catalase cholinesterase glutathione transferase glycogen protein reactive oxygen metabolite superoxide dismutase azinphos methyl carboxylesterase catalase cholinesterase fresh water glutathione transferase insecticide reactive oxygen metabolite superoxide dismutase antioxidant biomarker concentration (composition) gastropod insecticide invertebrate pollution exposure reproductive behavior toxicity adult antioxidant activity Argentina Article biochemical analysis Biomphalaria straminea chemical parameters concentration (parameters) controlled study environmental exposure enzyme activity hatching nonhuman protein analysis reproduction survival rate toxicity testing urogenital system parameters zygote animal Biomphalaria drug effect metabolism physiology toxicity water pollutant Argentina Biomphalaria straminea Gastropoda Invertebrata Animals Azinphosmethyl Biomarkers Biomphalaria Carboxylic Ester Hydrolases Catalase Cholinesterases Fresh Water Glutathione Transferase Insecticides Reactive Oxygen Species Reproduction Superoxide Dismutase Water Pollutants, Chemical Cossi, P.F. Herbert, L.T. Yusseppone, M.S. Pérez, A.F. Kristoff, G. Environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod Biomphalaria straminea |
| topic_facet |
Antioxidants B-esterases Biomarkers Insecticide Invertebrates Reproduction azinphos methyl biological marker carboxylesterase catalase cholinesterase glutathione transferase glycogen protein reactive oxygen metabolite superoxide dismutase azinphos methyl carboxylesterase catalase cholinesterase fresh water glutathione transferase insecticide reactive oxygen metabolite superoxide dismutase antioxidant biomarker concentration (composition) gastropod insecticide invertebrate pollution exposure reproductive behavior toxicity adult antioxidant activity Argentina Article biochemical analysis Biomphalaria straminea chemical parameters concentration (parameters) controlled study environmental exposure enzyme activity hatching nonhuman protein analysis reproduction survival rate toxicity testing urogenital system parameters zygote animal Biomphalaria drug effect metabolism physiology toxicity water pollutant Argentina Biomphalaria straminea Gastropoda Invertebrata Animals Azinphosmethyl Biomarkers Biomphalaria Carboxylic Ester Hydrolases Catalase Cholinesterases Fresh Water Glutathione Transferase Insecticides Reactive Oxygen Species Reproduction Superoxide Dismutase Water Pollutants, Chemical |
| description |
Organophosphate insecticides (OPs) are commonly used in Argentina and around the world for pest control in food crops. They exert their toxicity through the inhibition of the enzyme acetylcholinesterase. In the present study, we aimed to evaluate biochemical and reproductive effects in Biomphalaria straminea, a freshwater gastropod naturally distributed in Argentina, of subchronic exposures to environmental azinphos-methyl concentrations (20 and 200 µg L−1). For biochemical parameters, adult organisms were exposed for 14 days and the activity of cholinesterases (ChEs), carboxylesterases (CEs), glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), the production of reactive oxygen species (ROS), the total antioxidant capacity (TAC), glycogen and proteins were determined. For reproductive parameters, the egg masses of B. straminea were exposed to azinphos-methyl for one month, and the hatching time and success as well as the offspring survival were registered. We found different toxic effects elicited by the insecticide on the studied biomarkers. CEs activity was significantly inhibited while CAT and GST activities, ROS production and TAC were significantly increased, with respect to the solvent control group. ChE and SOD activities and protein and glycogen contents were not altered by azinphos-methyl. The hatching time and success were not statistically different from control. Nevertheless, the offspring survival was severely affected by the insecticide. Our results show that the primary target of the insecticide (ChE) was not inhibited but CEs, GST, CAT, ROS, TAC and offspring survival were sensitive biomarkers and valuable endpoints for subchronic toxicity assessments in this species. © 2018 Elsevier Inc. |
| format |
JOUR |
| author |
Cossi, P.F. Herbert, L.T. Yusseppone, M.S. Pérez, A.F. Kristoff, G. |
| author_facet |
Cossi, P.F. Herbert, L.T. Yusseppone, M.S. Pérez, A.F. Kristoff, G. |
| author_sort |
Cossi, P.F. |
| title |
Environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod Biomphalaria straminea |
| title_short |
Environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod Biomphalaria straminea |
| title_full |
Environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod Biomphalaria straminea |
| title_fullStr |
Environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod Biomphalaria straminea |
| title_full_unstemmed |
Environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod Biomphalaria straminea |
| title_sort |
environmental concentrations of azinphos-methyl cause different toxic effects without affecting the main target (cholinesterases) in the freshwater gastropod biomphalaria straminea |
| url |
http://hdl.handle.net/20.500.12110/paper_01476513_v162_n_p287_Cossi |
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