Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)

Endocrine disrupting compounds (EDCs) have the potential to alter fish reproduction at various levels of organization. The aim of this study was to assess the impact of a natural environment with heavily anthropogenic influence on the physiological processes involved in reproduction in the freshwate...

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Autores principales:	Tolussi, C.E., Gomes, A.D.O., Kumar, A., Ribeiro, C.S., Nostro, F.L.L., Bain, P.A., de Souza, G.B., Cuña, R.D., Honji, R.M., Moreira, R.G.
Formato:	JOUR
Materias:	Anthropogenic impact Characidae Endocrine disrupting compounds Estradiol Gene expression Oocyte maturation Vitellogenin endocrine disruptor estradiol estrogen receptor alpha vitellogenin biological marker fresh water anthropogenic effect biochemistry biomarker estrogenic compound gene expression maturation molecular analysis physiological response pollution effect teleost adult animal experiment animal tissue anthropometric parameters Astyanax fasciatus Brazil Characiformes controlled study estradiol blood level estrogen activity female fertility gonad development gonadosomatic index hepatosomatic index male nonhuman oocyte development ovary development pollution Review seasonal variation watershed analysis animal chemistry drug effects environmental monitoring gonad growth, development and aging metabolism organogenesis pathology procedures reproduction season toxicity water pollutant Teleostei Animals Biomarkers Endocrine Disruptors Environmental Monitoring Female Fertility Fresh Water Gene Expression Gonads Male Organogenesis Reproduction Seasons Vitellogenins Water Pollutants, Chemical
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01476513_v147_n_p926_Tolussi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01476513_v147_n_p926_Tolussi
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spelling	todo:paper_01476513_v147_n_p926_Tolussi2023-10-03T15:00:40Z Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae) Tolussi, C.E. Gomes, A.D.O. Kumar, A. Ribeiro, C.S. Nostro, F.L.L. Bain, P.A. de Souza, G.B. Cuña, R.D. Honji, R.M. Moreira, R.G. Anthropogenic impact Characidae Endocrine disrupting compounds Estradiol Gene expression Oocyte maturation Vitellogenin endocrine disruptor estradiol estrogen receptor alpha vitellogenin biological marker endocrine disruptor estradiol fresh water vitellogenin anthropogenic effect biochemistry biomarker endocrine disruptor estrogenic compound gene expression maturation molecular analysis physiological response pollution effect teleost adult animal experiment animal tissue anthropometric parameters Astyanax fasciatus Brazil Characidae Characiformes controlled study estradiol blood level estrogen activity female fertility gene expression gonad development gonadosomatic index hepatosomatic index male nonhuman oocyte development ovary development pollution Review seasonal variation teleost watershed analysis animal Characidae chemistry drug effects environmental monitoring gonad growth, development and aging metabolism organogenesis pathology procedures reproduction season toxicity water pollutant Astyanax fasciatus Characidae Characiformes Teleostei Animals Biomarkers Brazil Characidae Endocrine Disruptors Environmental Monitoring Estradiol Female Fertility Fresh Water Gene Expression Gonads Male Organogenesis Reproduction Seasons Vitellogenins Water Pollutants, Chemical Endocrine disrupting compounds (EDCs) have the potential to alter fish reproduction at various levels of organization. The aim of this study was to assess the impact of a natural environment with heavily anthropogenic influence on the physiological processes involved in reproduction in the freshwater fish lambari (Astyanax fasciatus) using different biomarkers. Adult males and females were collected in different seasons from two distinct sites in the same watershed: Ponte Nova Reservoir (PN) considered a pristine or small anthropogenic influence reference point; and Billings Reservoir (Bil), subjected to a large anthropogenic impact. Biological indices, such as hepatosomatic index and gonadosomatic index (GSI), gonadal histomorphology, fecundity, and biomarkers such as plasma levels of estradiol (E2) as well as hepatic gene expression of its alfa nuclear receptor (ERα), were analyzed. Hepatic vitellogenin (VTG) gene expression was evaluated in both sexes, as an indicator of xenoestrogen exposure. Females collected at PN presented a typical annual variation reflected in GSI, whereas for those sampled at Bil the index did not change through the seasons. The higher concentration of E2 in males collected at Bil during spring/2013, together with the detection of VTG gene expression, suggest the presence of EDCs in the water. These EDCs may have also influenced fecundity of females from Bil, which was higher during winter and spring/2013. Gene expression of ERα and ovarian morphology did not differ between fish from both sites. Water conditions from Bil reservoir impacted by anthropic activity clearly interfered mainly with biomarkers of biological effect such as plasma E2 levels and absolute and relative fecundity, but also altered biomarkers of exposure as VTG gene expression. These facts support the notion that waterborne EDCs are capable of causing estrogenic activity in A. fasciatus. © 2017 Elsevier Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v147_n_p926_Tolussi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Anthropogenic impact Characidae Endocrine disrupting compounds Estradiol Gene expression Oocyte maturation Vitellogenin endocrine disruptor estradiol estrogen receptor alpha vitellogenin biological marker endocrine disruptor estradiol fresh water vitellogenin anthropogenic effect biochemistry biomarker endocrine disruptor estrogenic compound gene expression maturation molecular analysis physiological response pollution effect teleost adult animal experiment animal tissue anthropometric parameters Astyanax fasciatus Brazil Characidae Characiformes controlled study estradiol blood level estrogen activity female fertility gene expression gonad development gonadosomatic index hepatosomatic index male nonhuman oocyte development ovary development pollution Review seasonal variation teleost watershed analysis animal Characidae chemistry drug effects environmental monitoring gonad growth, development and aging metabolism organogenesis pathology procedures reproduction season toxicity water pollutant Astyanax fasciatus Characidae Characiformes Teleostei Animals Biomarkers Brazil Characidae Endocrine Disruptors Environmental Monitoring Estradiol Female Fertility Fresh Water Gene Expression Gonads Male Organogenesis Reproduction Seasons Vitellogenins Water Pollutants, Chemical
spellingShingle	Anthropogenic impact Characidae Endocrine disrupting compounds Estradiol Gene expression Oocyte maturation Vitellogenin endocrine disruptor estradiol estrogen receptor alpha vitellogenin biological marker endocrine disruptor estradiol fresh water vitellogenin anthropogenic effect biochemistry biomarker endocrine disruptor estrogenic compound gene expression maturation molecular analysis physiological response pollution effect teleost adult animal experiment animal tissue anthropometric parameters Astyanax fasciatus Brazil Characidae Characiformes controlled study estradiol blood level estrogen activity female fertility gene expression gonad development gonadosomatic index hepatosomatic index male nonhuman oocyte development ovary development pollution Review seasonal variation teleost watershed analysis animal Characidae chemistry drug effects environmental monitoring gonad growth, development and aging metabolism organogenesis pathology procedures reproduction season toxicity water pollutant Astyanax fasciatus Characidae Characiformes Teleostei Animals Biomarkers Brazil Characidae Endocrine Disruptors Environmental Monitoring Estradiol Female Fertility Fresh Water Gene Expression Gonads Male Organogenesis Reproduction Seasons Vitellogenins Water Pollutants, Chemical Tolussi, C.E. Gomes, A.D.O. Kumar, A. Ribeiro, C.S. Nostro, F.L.L. Bain, P.A. de Souza, G.B. Cuña, R.D. Honji, R.M. Moreira, R.G. Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)
topic_facet	Anthropogenic impact Characidae Endocrine disrupting compounds Estradiol Gene expression Oocyte maturation Vitellogenin endocrine disruptor estradiol estrogen receptor alpha vitellogenin biological marker endocrine disruptor estradiol fresh water vitellogenin anthropogenic effect biochemistry biomarker endocrine disruptor estrogenic compound gene expression maturation molecular analysis physiological response pollution effect teleost adult animal experiment animal tissue anthropometric parameters Astyanax fasciatus Brazil Characidae Characiformes controlled study estradiol blood level estrogen activity female fertility gene expression gonad development gonadosomatic index hepatosomatic index male nonhuman oocyte development ovary development pollution Review seasonal variation teleost watershed analysis animal Characidae chemistry drug effects environmental monitoring gonad growth, development and aging metabolism organogenesis pathology procedures reproduction season toxicity water pollutant Astyanax fasciatus Characidae Characiformes Teleostei Animals Biomarkers Brazil Characidae Endocrine Disruptors Environmental Monitoring Estradiol Female Fertility Fresh Water Gene Expression Gonads Male Organogenesis Reproduction Seasons Vitellogenins Water Pollutants, Chemical
description	Endocrine disrupting compounds (EDCs) have the potential to alter fish reproduction at various levels of organization. The aim of this study was to assess the impact of a natural environment with heavily anthropogenic influence on the physiological processes involved in reproduction in the freshwater fish lambari (Astyanax fasciatus) using different biomarkers. Adult males and females were collected in different seasons from two distinct sites in the same watershed: Ponte Nova Reservoir (PN) considered a pristine or small anthropogenic influence reference point; and Billings Reservoir (Bil), subjected to a large anthropogenic impact. Biological indices, such as hepatosomatic index and gonadosomatic index (GSI), gonadal histomorphology, fecundity, and biomarkers such as plasma levels of estradiol (E2) as well as hepatic gene expression of its alfa nuclear receptor (ERα), were analyzed. Hepatic vitellogenin (VTG) gene expression was evaluated in both sexes, as an indicator of xenoestrogen exposure. Females collected at PN presented a typical annual variation reflected in GSI, whereas for those sampled at Bil the index did not change through the seasons. The higher concentration of E2 in males collected at Bil during spring/2013, together with the detection of VTG gene expression, suggest the presence of EDCs in the water. These EDCs may have also influenced fecundity of females from Bil, which was higher during winter and spring/2013. Gene expression of ERα and ovarian morphology did not differ between fish from both sites. Water conditions from Bil reservoir impacted by anthropic activity clearly interfered mainly with biomarkers of biological effect such as plasma E2 levels and absolute and relative fecundity, but also altered biomarkers of exposure as VTG gene expression. These facts support the notion that waterborne EDCs are capable of causing estrogenic activity in A. fasciatus. © 2017 Elsevier Inc.
format	JOUR
author	Tolussi, C.E. Gomes, A.D.O. Kumar, A. Ribeiro, C.S. Nostro, F.L.L. Bain, P.A. de Souza, G.B. Cuña, R.D. Honji, R.M. Moreira, R.G.
author_facet	Tolussi, C.E. Gomes, A.D.O. Kumar, A. Ribeiro, C.S. Nostro, F.L.L. Bain, P.A. de Souza, G.B. Cuña, R.D. Honji, R.M. Moreira, R.G.
author_sort	Tolussi, C.E.
title	Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)
title_short	Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)
title_full	Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)
title_fullStr	Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)
title_full_unstemmed	Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)
title_sort	environmental pollution affects molecular and biochemical responses during gonadal maturation of astyanax fasciatus (teleostei: characiformes: characidae)
url	http://hdl.handle.net/20.500.12110/paper_01476513_v147_n_p926_Tolussi
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Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)

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