Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxid...
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todo:paper_01476513_v120_n_p136_Sabatini2023-10-03T15:00:38Z Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata Sabatini, S.E. Brena, B.M. Pirez, M. de Molina, M.D.C.R. Luquet, C.M. ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor glutathione glutathione transferase microcystin superoxide dismutase water pollutant antioxidant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain bioaccumulation clinical trial (topic) controlled study crab detoxification enzyme activity excretion hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects hepatopancreas lipid peroxidation metabolism Microcystis oxidative stress pharmacokinetics physiology toxicity water pollutant Chasmagnathus Decapoda (Crustacea) Microcystis aeruginosa Animals Antioxidants Brachyura Enzyme Inhibitors Glutathione Glutathione Transferase Hepatopancreas Lipid Peroxidation Microcystins Microcystis Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication. © 2015 Elsevier Inc. Fil:Sabatini, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v120_n_p136_Sabatini |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor glutathione glutathione transferase microcystin superoxide dismutase water pollutant antioxidant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain bioaccumulation clinical trial (topic) controlled study crab detoxification enzyme activity excretion hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects hepatopancreas lipid peroxidation metabolism Microcystis oxidative stress pharmacokinetics physiology toxicity water pollutant Chasmagnathus Decapoda (Crustacea) Microcystis aeruginosa Animals Antioxidants Brachyura Enzyme Inhibitors Glutathione Glutathione Transferase Hepatopancreas Lipid Peroxidation Microcystins Microcystis Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical |
| spellingShingle |
ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor glutathione glutathione transferase microcystin superoxide dismutase water pollutant antioxidant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain bioaccumulation clinical trial (topic) controlled study crab detoxification enzyme activity excretion hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects hepatopancreas lipid peroxidation metabolism Microcystis oxidative stress pharmacokinetics physiology toxicity water pollutant Chasmagnathus Decapoda (Crustacea) Microcystis aeruginosa Animals Antioxidants Brachyura Enzyme Inhibitors Glutathione Glutathione Transferase Hepatopancreas Lipid Peroxidation Microcystins Microcystis Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical Sabatini, S.E. Brena, B.M. Pirez, M. de Molina, M.D.C.R. Luquet, C.M. Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata |
| topic_facet |
ELISA Estuarine crab Hepatopancreas Microcystin depuration Toxin bioaccumulation glutathione glutathione transferase microcystin LR superoxide dismutase thiobarbituric acid reactive substance antioxidant enzyme inhibitor glutathione glutathione transferase microcystin superoxide dismutase water pollutant antioxidant bioaccumulation crab detoxification diet ecotoxicology estuarine ecosystem excretion phytotoxicity toxin adult animal experiment animal tissue antioxidant activity Article bacterial strain bioaccumulation clinical trial (topic) controlled study crab detoxification enzyme activity excretion hepatopancreas host resistance long term exposure male Microcystis aeruginosa Neohelice granulata nonhuman oxidative stress toxin analysis xenobiotic metabolism animal Brachyura drug effects hepatopancreas lipid peroxidation metabolism Microcystis oxidative stress pharmacokinetics physiology toxicity water pollutant Chasmagnathus Decapoda (Crustacea) Microcystis aeruginosa Animals Antioxidants Brachyura Enzyme Inhibitors Glutathione Glutathione Transferase Hepatopancreas Lipid Peroxidation Microcystins Microcystis Oxidative Stress Superoxide Dismutase Water Pollutants, Chemical |
| description |
We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication. © 2015 Elsevier Inc. |
| format |
JOUR |
| author |
Sabatini, S.E. Brena, B.M. Pirez, M. de Molina, M.D.C.R. Luquet, C.M. |
| author_facet |
Sabatini, S.E. Brena, B.M. Pirez, M. de Molina, M.D.C.R. Luquet, C.M. |
| author_sort |
Sabatini, S.E. |
| title |
Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata |
| title_short |
Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata |
| title_full |
Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata |
| title_fullStr |
Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata |
| title_full_unstemmed |
Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata |
| title_sort |
oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab neohelice (chasmagnathus) granulata |
| url |
http://hdl.handle.net/20.500.12110/paper_01476513_v120_n_p136_Sabatini |
| work_keys_str_mv |
AT sabatinise oxidativeeffectsandtoxinbioaccumulationafterdietarymicrocystinintoxicationinthehepatopancreasofthecrabneohelicechasmagnathusgranulata AT brenabm oxidativeeffectsandtoxinbioaccumulationafterdietarymicrocystinintoxicationinthehepatopancreasofthecrabneohelicechasmagnathusgranulata AT pirezm oxidativeeffectsandtoxinbioaccumulationafterdietarymicrocystinintoxicationinthehepatopancreasofthecrabneohelicechasmagnathusgranulata AT demolinamdcr oxidativeeffectsandtoxinbioaccumulationafterdietarymicrocystinintoxicationinthehepatopancreasofthecrabneohelicechasmagnathusgranulata AT luquetcm oxidativeeffectsandtoxinbioaccumulationafterdietarymicrocystinintoxicationinthehepatopancreasofthecrabneohelicechasmagnathusgranulata |
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1807314411201757184 |