Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus
A polysaccharide with the properties of glycogen has been isolated by water or phenol-water extraction from homogenates of Ascobolus furfuraceus. After 4 days of growth a maximum glycogen content of 28% of the cell dry weight was found by a specific enzymic method or by the iodine method. Taking int...
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| Autores principales: | , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01475975_v5_n3_p253_Groisman |
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| Sumario: | A polysaccharide with the properties of glycogen has been isolated by water or phenol-water extraction from homogenates of Ascobolus furfuraceus. After 4 days of growth a maximum glycogen content of 28% of the cell dry weight was found by a specific enzymic method or by the iodine method. Taking into account the cell growth, the highest total amount of glycogen (2.04 mg/ml of broth) occurred on the 10th day. The average chain length determined by periodate oxidation was about 11. This result shows that the reserve polysaccharide from A. furfuraceus has a highly branched structure similar to liver glycogen. © 1981. |
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