Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus

A polysaccharide with the properties of glycogen has been isolated by water or phenol-water extraction from homogenates of Ascobolus furfuraceus. After 4 days of growth a maximum glycogen content of 28% of the cell dry weight was found by a specific enzymic method or by the iodine method. Taking int...

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Autores principales: Groisman, J.F., Forchiassin, F., Ranalli, M.E., de Lederkremer, R.M.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01475975_v5_n3_p253_Groisman
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spelling todo:paper_01475975_v5_n3_p253_Groisman2023-10-03T15:00:34Z Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus Groisman, J.F. Forchiassin, F. Ranalli, M.E. de Lederkremer, R.M. Ascobolus furfuraceus coprophilous fungus glycogen characteristics A polysaccharide with the properties of glycogen has been isolated by water or phenol-water extraction from homogenates of Ascobolus furfuraceus. After 4 days of growth a maximum glycogen content of 28% of the cell dry weight was found by a specific enzymic method or by the iodine method. Taking into account the cell growth, the highest total amount of glycogen (2.04 mg/ml of broth) occurred on the 10th day. The average chain length determined by periodate oxidation was about 11. This result shows that the reserve polysaccharide from A. furfuraceus has a highly branched structure similar to liver glycogen. © 1981. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01475975_v5_n3_p253_Groisman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ascobolus furfuraceus
coprophilous fungus
glycogen characteristics
spellingShingle Ascobolus furfuraceus
coprophilous fungus
glycogen characteristics
Groisman, J.F.
Forchiassin, F.
Ranalli, M.E.
de Lederkremer, R.M.
Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus
topic_facet Ascobolus furfuraceus
coprophilous fungus
glycogen characteristics
description A polysaccharide with the properties of glycogen has been isolated by water or phenol-water extraction from homogenates of Ascobolus furfuraceus. After 4 days of growth a maximum glycogen content of 28% of the cell dry weight was found by a specific enzymic method or by the iodine method. Taking into account the cell growth, the highest total amount of glycogen (2.04 mg/ml of broth) occurred on the 10th day. The average chain length determined by periodate oxidation was about 11. This result shows that the reserve polysaccharide from A. furfuraceus has a highly branched structure similar to liver glycogen. © 1981.
format JOUR
author Groisman, J.F.
Forchiassin, F.
Ranalli, M.E.
de Lederkremer, R.M.
author_facet Groisman, J.F.
Forchiassin, F.
Ranalli, M.E.
de Lederkremer, R.M.
author_sort Groisman, J.F.
title Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus
title_short Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus
title_full Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus
title_fullStr Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus
title_full_unstemmed Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus
title_sort isolation and characterization of glycogen from mycelium of ascobolus furfuraceus
url http://hdl.handle.net/20.500.12110/paper_01475975_v5_n3_p253_Groisman
work_keys_str_mv AT groismanjf isolationandcharacterizationofglycogenfrommyceliumofascobolusfurfuraceus
AT forchiassinf isolationandcharacterizationofglycogenfrommyceliumofascobolusfurfuraceus
AT ranallime isolationandcharacterizationofglycogenfrommyceliumofascobolusfurfuraceus
AT delederkremerrm isolationandcharacterizationofglycogenfrommyceliumofascobolusfurfuraceus
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