A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level...
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| Autores principales: | , , , |
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| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01475975_v13_n2_p121_Aisemberg |
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| Sumario: | In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level for a long period. An open coding region for a 70-kDa protein was demonstrated by hybrid-selected translation, in agreement with the in vivo labeling of a protein with the same mobility in cycloheximide-induced cultures. Even 48 h after cycloheximide addition, CI-1-specific mRNA was bound to polysomal fractions. Our results also suggest that DNA sequences analogous to N. crassa CI-1 gene are present in other species. In N. crassa, CI-1 gene would not be induced by heat shock or starvation. © 1989. |
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