A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide

In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level...

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Autores principales:	Aisemberg, G.O., Grotewold, E., Taccioli, G.E., Judewicz, N.D.
Formato:	JOUR
Materias:	cycloheximide gene expression Neurospora crassa stress proteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01475975_v13_n2_p121_Aisemberg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01475975_v13_n2_p121_Aisemberg2023-10-03T15:00:30Z A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide Aisemberg, G.O. Grotewold, E. Taccioli, G.E. Judewicz, N.D. cycloheximide gene expression Neurospora crassa stress proteins In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level for a long period. An open coding region for a 70-kDa protein was demonstrated by hybrid-selected translation, in agreement with the in vivo labeling of a protein with the same mobility in cycloheximide-induced cultures. Even 48 h after cycloheximide addition, CI-1-specific mRNA was bound to polysomal fractions. Our results also suggest that DNA sequences analogous to N. crassa CI-1 gene are present in other species. In N. crassa, CI-1 gene would not be induced by heat shock or starvation. © 1989. Fil:Aisemberg, G.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grotewold, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Taccioli, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Judewicz, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01475975_v13_n2_p121_Aisemberg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cycloheximide gene expression Neurospora crassa stress proteins
spellingShingle	cycloheximide gene expression Neurospora crassa stress proteins Aisemberg, G.O. Grotewold, E. Taccioli, G.E. Judewicz, N.D. A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
topic_facet	cycloheximide gene expression Neurospora crassa stress proteins
description	In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level for a long period. An open coding region for a 70-kDa protein was demonstrated by hybrid-selected translation, in agreement with the in vivo labeling of a protein with the same mobility in cycloheximide-induced cultures. Even 48 h after cycloheximide addition, CI-1-specific mRNA was bound to polysomal fractions. Our results also suggest that DNA sequences analogous to N. crassa CI-1 gene are present in other species. In N. crassa, CI-1 gene would not be induced by heat shock or starvation. © 1989.
format	JOUR
author	Aisemberg, G.O. Grotewold, E. Taccioli, G.E. Judewicz, N.D.
author_facet	Aisemberg, G.O. Grotewold, E. Taccioli, G.E. Judewicz, N.D.
author_sort	Aisemberg, G.O.
title	A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
title_short	A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
title_full	A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
title_fullStr	A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
title_full_unstemmed	A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
title_sort	major transcript in the response of neurospora crassa to protein synthesis inhibition by cycloheximide
url	http://hdl.handle.net/20.500.12110/paper_01475975_v13_n2_p121_Aisemberg
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A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide

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